1992
A glutamine residue in the membrane-associating domain of the bovine papillomavirus type 1 E5 oncoprotein mediates its binding to a transmembrane component of the vacuolar H(+)-ATPase
Goldstein D, Kulke R, Dimaio D, Schlegel R. A glutamine residue in the membrane-associating domain of the bovine papillomavirus type 1 E5 oncoprotein mediates its binding to a transmembrane component of the vacuolar H(+)-ATPase. Journal Of Virology 1992, 66: 405-413. PMID: 1370089, PMCID: PMC238300, DOI: 10.1128/jvi.66.1.405-413.1992.Peer-Reviewed Original ResearchConceptsK proteinGrowth factor receptorE5 oncoproteinGlutamine residuesRandom hydrophobic sequencesSpecific amino acid residuesMembrane-associated domainMajor transforming proteinK protein componentCarboxyl-terminal domainEndoplasmic reticulum membraneFactor receptorBovine papillomavirus type 1Colony-stimulating factor 1 receptorTransformation-defective mutantsAmino acid residuesPotential binding sitesPlatelet-derived growth factor receptorAmino acid substitutionsPapillomavirus type 1Hydrophilic amino acidsE5 dimerE5 mutantsFactor 1 receptorProtein complexes
1990
Integrated HPV 1 genomes in a human keratinocyte cell line can be transactivated by a SV40/BPV1 recombinant virus which expresses BPV1 E2 proteins
Partow A, Grand R, Biggs P, Jeffrey S, Dimaio D, Gallimore P. Integrated HPV 1 genomes in a human keratinocyte cell line can be transactivated by a SV40/BPV1 recombinant virus which expresses BPV1 E2 proteins. Virology 1990, 175: 508-517. PMID: 2158183, DOI: 10.1016/0042-6822(90)90435-t.Peer-Reviewed Original ResearchCell Line, TransformedClone CellsDNA, RecombinantDNA, ViralDNA-Binding ProteinsGene Expression Regulation, ViralHumansKaryotypingKeratinocytesNucleic Acid HybridizationPapillomaviridaePolymerase Chain ReactionRecombinant ProteinsRNA SplicingSimian virus 40Transcription, GeneticTranscriptional ActivationTransfectionViral ProteinsBovine papillomavirus E2 repressor mutant displays a high-copy-number phenotype and enhanced transforming activity
Riese D, Settleman J, Neary K, DiMaio D. Bovine papillomavirus E2 repressor mutant displays a high-copy-number phenotype and enhanced transforming activity. Journal Of Virology 1990, 64: 944-949. PMID: 2153255, PMCID: PMC249196, DOI: 10.1128/jvi.64.2.944-949.1990.Peer-Reviewed Original ResearchConceptsRepressor proteinWild-type copy numberBovine papillomavirus type 1 genomeCopy numberViral genomeTranscriptional repressor proteinTransient expression experimentsMouse C127 cellsMutant viral DNAViral DNAType 1 genomeNumber phenotypeRepressor activityLow copy numberMethionine codonInitiation codonExpression experimentsC127 cellsGenomeCV1 cellsRepressor mutantsFoci formationColony formationCodonProtein[45] Saturation mutagenesis using mixed oligonucleotides and M13 templates containing uracil
Horwitz B, Dimaio D. [45] Saturation mutagenesis using mixed oligonucleotides and M13 templates containing uracil. Methods In Enzymology 1990, 185: 599-611. PMID: 2166223, DOI: 10.1016/0076-6879(90)85047-r.Peer-Reviewed Original Research
1989
Open reading frames E6 and E7 of bovine papillomavirus type 1 are both required for full transformation of mouse C127 cells
Neary K, DiMaio D. Open reading frames E6 and E7 of bovine papillomavirus type 1 are both required for full transformation of mouse C127 cells. Journal Of Virology 1989, 63: 259-266. PMID: 2535732, PMCID: PMC247680, DOI: 10.1128/jvi.63.1.259-266.1989.Peer-Reviewed Original ResearchConceptsBovine papillomavirus type 1Open reading frames E6Mouse C127 cellsFull-length viral genomesAnchorage-independent growthPapillomavirus type 1Focus-forming activityC127 cellsORF E6First methionine codonViral genomeColony formationRetrovirus long terminal repeatsSeries of mutationsE6/E7Second ATG codonLong terminal repeatBPV1 genomeMethionine codonATG codonNumber plasmidE5 geneSpecific proteinsSimultaneous disruptionE6 protein
1987
Stimulation of cellular DNA synthesis by wild type and mutant bovine papillomavirus DNA
Jaskulski D, Kaczmarek L, DiMaio D. Stimulation of cellular DNA synthesis by wild type and mutant bovine papillomavirus DNA. Biochemical And Biophysical Research Communications 1987, 148: 86-91. PMID: 2823817, DOI: 10.1016/0006-291x(87)91079-5.Peer-Reviewed Original Research
1986
Nonsense mutation in open reading frame E2 of bovine papillomavirus DNA
DiMaio D. Nonsense mutation in open reading frame E2 of bovine papillomavirus DNA. Journal Of Virology 1986, 57: 475-480. PMID: 3003380, PMCID: PMC252759, DOI: 10.1128/jvi.57.2.475-480.1986.Peer-Reviewed Original ResearchConceptsMutant viral DNAOpen reading frame E2C127 cellsBovine papillomavirus DNAViral DNAGenetic mapping experimentsNonsense mutationMouse C127 cellsSingle base substitution mutationsHost cell genomeFull-length viral DNAFocus-forming activityBase substitution mutationsWild-type DNAAmber mutationCell genomeOncogenic transformationViral ORFsSubstitution mutationsORF E2Mapping experimentsMutant DNATransformation assaysDNABovine papillomavirus
1984
High-level expression of a cloned HLA heavy chain gene introduced into mouse cells on a bovine papillomavirus vector.
DiMaio D, Corbin V, Sibley E, Maniatis T. High-level expression of a cloned HLA heavy chain gene introduced into mouse cells on a bovine papillomavirus vector. Molecular And Cellular Biology 1984, 4: 340-350. PMID: 6321959, PMCID: PMC368701, DOI: 10.1128/mcb.4.2.340.Peer-Reviewed Original ResearchConceptsHLA antigensBovine papillomavirus vectorCell linesFunction of HLA antigensHuman heavy chainsBovine papillomavirusHeavy chainHuman lymphoblastoid cell linesHeavy chain geneMouse cellsDNA expression vectorsLymphoblastoid cell linesTransformed cellsJY cell lineExtrachromosomal viral DNAHuman histocompatibility antigensSelection in vivoHistocompatibility antigensTransformed cell linesHLA genesChain geneCultured mouse cellsHLAHigh-level expressionAntigen
1982
Bovine papillomavirus vector that propagates as a plasmid in both mouse and bacterial cells.
DiMaio D, Treisman R, Maniatis T. Bovine papillomavirus vector that propagates as a plasmid in both mouse and bacterial cells. Proceedings Of The National Academy Of Sciences Of The United States Of America 1982, 79: 4030-4034. PMID: 6287462, PMCID: PMC346570, DOI: 10.1073/pnas.79.13.4030.Peer-Reviewed Original ResearchConceptsMammalian cellsBacterial cellsInduce focus formationHuman beta-globin gene clusterBeta-globin gene clusterBPV DNADerivative of pBR322Mouse C127 cellsTransformation of bacteriaTransformed mouse cellsHuman beta-globin geneExtrachromosomal elementsGene clusterBeta-globin geneBovine papillomavirus vectorDeletion derivativesInput DNACellular genesRecombinant plasmidC127 cellsCopy numberMouse cellsTransformed cellsPlasmidFoci formationRegulatory mutants of simian virus 40 Effect of mutations at a T antigen binding site on DNA replication and expression of viral genes
DiMaio D, Nathans D. Regulatory mutants of simian virus 40 Effect of mutations at a T antigen binding site on DNA replication and expression of viral genes. Journal Of Molecular Biology 1982, 156: 531-548. PMID: 6288959, DOI: 10.1016/0022-2836(82)90265-0.Peer-Reviewed Original ResearchConceptsViral DNA replicationDNA replicationEarly gene transcriptionT antigenGene transcriptionGene expressionCold-sensitive defectSecond-site mutationsSegment of DNAT antigen functionLate protein synthesisSimian virus 40 mutantsSV40 early proteinsLarge T antigenT-antigen geneEarly gene expressionBase pair substitutionsRegulatory mutantsRegulatory segmentRegulatory regionsDefined deletionsViral regulatory regionViral genesMutantsDNA show
1981
Binding of an SV40 T antigen-related protein to the DNA of SV40 regulatory mutants
McKay R, DiMaio D. Binding of an SV40 T antigen-related protein to the DNA of SV40 regulatory mutants. Nature 1981, 289: 810-813. PMID: 6258090, DOI: 10.1038/289810a0.Peer-Reviewed Original Research
1980
Cold-sensitive regulatory mutants of simian virus 40
DiMaio D, Nathans D. Cold-sensitive regulatory mutants of simian virus 40. Journal Of Molecular Biology 1980, 140: 129-142. PMID: 6251230, DOI: 10.1016/0022-2836(80)90359-9.Peer-Reviewed Original ResearchConceptsRegulatory segmentBase substitutionsBglI siteWild-type plaquesProtein-coding sequencesProtein-nucleic acid interactionsCold-sensitive mutantsOrigin of replicationSet of mutantsSimian virus 40 mutantsDNA polymerase IBase substitution mutationsRNA processingRegulatory mutantsDNA replicationReplication originsViable mutantsSimian virus 40Small plaquesMutational alterationsWild-type virusPolymerase IMutantsRegulatory phenomenaSubstitution mutations