Featured Publications
Evidence for phospholipid export from the bacterial inner membrane by the Mla ABC transport system
Hughes G, Hall S, Laxton C, Sridhar P, Mahadi A, Hatton C, Piggot T, Wotherspoon P, Leney A, Ward D, Jamshad M, Spana V, Cadby I, Harding C, Isom G, Bryant J, Parr R, Yakub Y, Jeeves M, Huber D, Henderson I, Clifton L, Lovering A, Knowles T. Evidence for phospholipid export from the bacterial inner membrane by the Mla ABC transport system. Nature Microbiology 2019, 4: 1692-1705. PMID: 31235958, DOI: 10.1038/s41564-019-0481-y.Peer-Reviewed Original ResearchConceptsMla pathwayInner membraneAsymmetric Gram-negative outer membranePhospholipid transportRetrograde phospholipid transportGram-negative outer membranePhospholipid-binding pocketABC transport systemSoluble periplasmic proteinBacterial inner membraneInner membrane ATPaseIndependent of ATPClosed apo conformationPhospholipid exportPeriplasmic proteinsMlaFEDB complexOuter membraneApo formApo conformationLipid exportMembrane ATPaseExport processPathwayMembraneMLAC
2021
Surface-tethered planar membranes containing the β-barrel assembly machinery: a platform for investigating bacterial outer membrane protein folding
Hall S, Clifton L, Sridhar P, Hardy D, Wotherspoon P, Wright J, Whitehouse J, Gamage N, Laxton C, Hatton C, Hughes G, Jeeves M, Knowles T. Surface-tethered planar membranes containing the β-barrel assembly machinery: a platform for investigating bacterial outer membrane protein folding. Biophysical Journal 2021, 120: 5295-5308. PMID: 34757080, PMCID: PMC8715194, DOI: 10.1016/j.bpj.2021.10.033.Peer-Reviewed Original ResearchConceptsOuter membrane proteinsBAM complexOuter membraneInserts outer membrane proteinsOuter membrane protein functionsOuter membrane protein foldingOuter membrane of Gram-negative bacteriaMembrane of Gram-negative bacteriaMembrane protein foldingQuartz crystal microbalanceSelf-assembled monolayersGram-negative bacteriaPhospholipid bilayer environmentAssembly machineryProtein foldingCrystal microbalanceMembrane proteinsGold surfaceStructural characterizationBiological functionsPolarized neutron reflectometryDissipation monitoringBilayer environmentComplex foldingProtein