2018
Transfused platelets enhance alloimmune responses to transfused KEL-expressing red blood cells in a murine model.
Madrid DJ, Santhanakrishnan M, Liu J, Gibb DR, Liu D, Natarajan P, Beitler D, Shi Z, Mo C, Tormey CA, Patel SR, Stowell SR, Hendrickson JE. Transfused platelets enhance alloimmune responses to transfused KEL-expressing red blood cells in a murine model. Blood Transfusion 2018, 17: 368-377. PMID: 30418129, PMCID: PMC6774925, DOI: 10.2450/2018.0178-18.Peer-Reviewed Original ResearchConceptsRed blood cell transfusionBlood cell transfusionTransfusion of plateletsAlloimmune responseRed blood cellsCell transfusionWild-type recipientsBlood cellsImmune responseMurine modelRed blood cell alloimmunisationCD40/CD40L interactionHuman KEL glycoproteinWild-type C57BL/6Humoral immune responseAdaptive immune responsesDevelopment of alloantibodiesPlatelet-rich plasmaEndogenous plateletsCD40L interactionRecipient treatmentTransfusionDanger signalsCD40LTransgenic donors
2016
Antigen modulation as a potential mechanism of anti-KEL immunoprophylaxis in mice
Liu J, Santhanakrishnan M, Natarajan P, Gibb DR, Eisenbarth SC, Tormey CA, Siddon AJ, Stowell SR, Branch DR, Hendrickson JE. Antigen modulation as a potential mechanism of anti-KEL immunoprophylaxis in mice. Blood 2016, 128: 3159-3168. PMID: 27688803, PMCID: PMC5201095, DOI: 10.1182/blood-2016-06-724732.Peer-Reviewed Original ResearchConceptsMechanism of actionKO miceRed blood cell alloimmunizationGlycoprotein antigenMurine RBCsHuman KEL glycoproteinRh immune globulinSingle knockout miceWild-type recipientsImmunoprophylaxis programKO recipientsPrevent alloimmunizationImmune globulinSerious complicationsAntigen modulationMurine modelImmunoprophylaxisFcγ receptorsKEL antigenAlloimmunizationFlow cytometryRapid clearanceWestern blotMiceAntigenClinically significant anti‐KEL RBC alloantibodies are transferred by breast milk in a murine model
Santhanakrishnan M, Tormey CA, Natarajan P, Liu J, Hendrickson JE. Clinically significant anti‐KEL RBC alloantibodies are transferred by breast milk in a murine model. Vox Sanguinis 2016, 111: 79-87. PMID: 26950259, PMCID: PMC4938760, DOI: 10.1111/vox.12387.Peer-Reviewed Original ResearchConceptsBreast milkRBC alloantibodiesIgA antibodiesMurine modelHuman KEL glycoproteinSeries of pregnanciesBreast milk transferHuman breast milkWild-type pupsKEL RBCsNursing infantsClinical significanceAlloantibodiesNursing pupsMurine dataTransfusion experimentsMilk transferInfantsPupsTransfusionHuman dataSequelaePregnancyAnemiaPremature clearance
2015
Riboflavin‐ultraviolet light pathogen reduction treatment does not impact the immunogenicity of murine red blood cells
Tormey CA, Santhanakrishnan M, Smith NH, Liu J, Marschner S, Goodrich RP, Hendrickson JE. Riboflavin‐ultraviolet light pathogen reduction treatment does not impact the immunogenicity of murine red blood cells. Transfusion 2015, 56: 863-872. PMID: 26643781, DOI: 10.1111/trf.13432.Peer-Reviewed Original ResearchConceptsWhite blood cellsFresh whole bloodPathogen reduction treatmentAlloimmune responseWhole bloodRBC antigensSyngeneic RBCsNegative direct antiglobulin testBlood cellsRBC antigen expressionRecipient alloimmune responsesDirect antiglobulin testMurine red blood cellsPathogen inactivation treatmentPosttransfusion RBC recoveryKEL RBCsRBC alloimmunizationImmunogenic neoantigensRed blood cellsAntiglobulin testBlood donorsImmune responseAntigen expressionHours posttransfusionHuman antigensAnti-KEL sera prevents alloimmunization to transfused KEL RBCs in a murine model
Stowell SR, Arthur CM, Girard-Pierce KR, Sullivan HC, Santhanakrishnan M, Natarajan P, Patel SR, Tormey CA, Zimring JC, Hendrickson JE. Anti-KEL sera prevents alloimmunization to transfused KEL RBCs in a murine model. Haematologica 2015, 100: e394-e397. PMID: 26185172, PMCID: PMC4591773, DOI: 10.3324/haematol.2015.128603.Peer-Reviewed Original Research