2013
Host-derived CD8+ dendritic cells are required for induction of optimal graft-versus-tumor responses after experimental allogeneic bone marrow transplantation
Toubai T, Sun Y, Luker G, Liu J, Luker K, Tawara I, Evers R, Liu C, Mathewson N, Malter C, Nieves E, Choi S, Murphy K, Reddy P. Host-derived CD8+ dendritic cells are required for induction of optimal graft-versus-tumor responses after experimental allogeneic bone marrow transplantation. Blood 2013, 121: 4231-4241. PMID: 23520337, PMCID: PMC3656455, DOI: 10.1182/blood-2012-05-432872.Peer-Reviewed Original ResearchConceptsAntigen-presenting cellsTumor-specific antigensGVT responseAllo-HCTAPC subsetsDendritic cellsExperimental allogeneic bone marrow transplantationHost-derived antigen-presenting cellsAllogeneic bone marrow transplantationAllogeneic hematopoietic cell transplantationAlloantigen-specific responsesHost-derived CD8Donor T cellsHematopoietic cell transplantationBone marrow transplantationRelevant murine modelStimulation of TLR3Host diseaseTumor effectMarrow transplantationCell transplantationTumor responseSerious toxicityT cellsOptimal graft
2012
Iron Regulator Hepcidin Exhibits Antiviral Activity against Hepatitis C Virus
Liu H, Le Trinh T, Dong H, Keith R, Nelson D, Liu C. Iron Regulator Hepcidin Exhibits Antiviral Activity against Hepatitis C Virus. PLOS ONE 2012, 7: e46631. PMID: 23110054, PMCID: PMC3478283, DOI: 10.1371/journal.pone.0046631.Peer-Reviewed Original ResearchConceptsHCV infectionHepcidin expressionSerious chronic liver diseaseAntiviral activityHepatitis C viral infectionHepcidin expression levelsChronic HCV infectionChronic liver diseaseC viral infectionHepatitis C virusDirect antiviral activityIntracellular antiviral stateHepatocellular carcinoma progressionInnate antiviral defenseLiver diseaseC virusAntiviral effectHCV replicationIron overloadHepatocellular carcinomaExhibit antiviral activityHCVViral infectionSuppressive effectAmino acid peptide
2011
Induction of acute GVHD by sex-mismatched H-Y antigens in the absence of functional radiosensitive host hematopoietic–derived antigen-presenting cells
Toubai T, Tawara I, Sun Y, Liu C, Nieves E, Evers R, Friedman T, Korngold R, Reddy P. Induction of acute GVHD by sex-mismatched H-Y antigens in the absence of functional radiosensitive host hematopoietic–derived antigen-presenting cells. Blood 2011, 119: 3844-3853. PMID: 22101894, PMCID: PMC3335388, DOI: 10.1182/blood-2011-10-384057.Peer-Reviewed Original ResearchMeSH KeywordsAcute DiseaseAnimalsAntigen-Presenting CellsBone Marrow TransplantationCD4-Positive T-LymphocytesCD8-Positive T-LymphocytesCells, CulturedDendritic CellsEndothelial CellsFemaleGraft vs Host DiseaseHematopoiesisHistocompatibilityH-Y AntigenIsoantigensMaleMiceMice, Inbred BALB CMice, Inbred C57BLMice, Mutant StrainsRadiation ToleranceThymectomyConceptsAcute GVHDT cellsAg presentationAlloreactive donor T cellsAllogeneic BM transplantationDonor T cellsMinor histocompatibility AgAntigen-presenting cellsGvH responseGVHD lethalityBM transplantationHistocompatibility AgClinical dataGVHDY antigenAPCY AgPresentationCellsTransplantationAbsenceInfusionAntigenRecipientsPrevention of GVHD while sparing GVL effect by targeting Th1 and Th17 transcription factor T-bet and RORγt in mice
Yu Y, Wang D, Liu C, Kaosaard K, Semple K, Anasetti C, Yu X. Prevention of GVHD while sparing GVL effect by targeting Th1 and Th17 transcription factor T-bet and RORγt in mice. Blood 2011, 118: 5011-5020. PMID: 21856864, PMCID: PMC3208306, DOI: 10.1182/blood-2011-03-340315.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntineoplastic AgentsCells, CulturedCombined Modality TherapyGraft vs Host DiseaseGraft vs Leukemia EffectHematopoietic Stem Cell TransplantationLeukemiaMiceMice, Inbred C57BLMice, KnockoutMolecular Targeted TherapyNuclear Receptor Subfamily 1, Group F, Member 3T-Box Domain ProteinsTh1 CellsTh17 CellsTransplantation, HomologousConceptsHematopoietic cell transplantationGVL effectT-betT cellsTranscription factor T-betPrevention of GVHDDonor T cellsCD8 T cellsAmeliorated GVHDGVL activityNaive ThTh17 subsetAdoptive transferCell transplantationTh17 differentiationEffective therapyHematologic malignanciesAllogeneic hostsGVHDMajor MHCTh1Regulatory phenotypeSkewed differentiationTargeted disruptionRORγtIkaros-Notch axis in host hematopoietic cells regulates experimental graft-versus-host disease
Toubai T, Sun Y, Tawara I, Friedman A, Liu C, Evers R, Nieves E, Malter C, Chockley P, Maillard I, Winandy S, Reddy P. Ikaros-Notch axis in host hematopoietic cells regulates experimental graft-versus-host disease. Blood 2011, 118: 192-204. PMID: 21471527, PMCID: PMC3139384, DOI: 10.1182/blood-2010-12-324616.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigen-Presenting CellsCD8-Positive T-LymphocytesCells, CulturedDendritic CellsDisease Models, AnimalFemaleGraft vs Host DiseaseHematopoiesisIkaros Transcription FactorImmunophenotypingMaleMiceMice, Inbred BALB CMice, Inbred C3HMice, Inbred C57BLMice, Mutant StrainsReceptor, Notch1Signal TransductionConceptsDendritic cellsAcute GVHDDonor T cell expansionExperimental bone marrow transplantationHost hematopoietic cellsInduction of GVHDAllogeneic T cellsBone marrow transplantationT cell expansionRelevant murine modelGvH responseAllostimulatory capacityHost diseaseWild-type controlsDC biologyMarrow transplantationPotent APCsActivation markersTLR stimulationT cellsMurine modelGVHDExperimental graftEnhanced stimulationIkaros deficiency
2010
Influence of Serum and Soluble CD25 (sCD25) on Regulatory and Effector T‐cell Function in Hepatocellular Carcinoma
Cabrera R, Ararat M, Eksioglu E, Cao M, Xu Y, Wasserfall C, Atkinson M, Liu C, Nelson D. Influence of Serum and Soluble CD25 (sCD25) on Regulatory and Effector T‐cell Function in Hepatocellular Carcinoma. Scandinavian Journal Of Immunology 2010, 72: 293-301. PMID: 20883314, PMCID: PMC2951624, DOI: 10.1111/j.1365-3083.2010.02427.x.Peer-Reviewed Original ResearchConceptsHepatocellular carcinomaHCC serumSerum-free mediumSoluble CD25Pg/Effector T cell responsesEffector T cell functionSerum-mediated suppressionRegulatory T cellsT cell responsesSera of patientsT cell functionHealthy control cellsNovel therapeutic targetDose-dependent fashionCD25 cell surface expressionTeff responsesTreg suppressionInfluence of serumCirrhosis patientsTeff proliferationTumor burdenCD25 expressionCell surface expressionPoor survival
2009
Hepatitis C virus modulates human monocyte‐derived dendritic cells
Eksioglu E, Bess J, Zhu H, Xu Y, Dong H, Elyar J, Nelson D, Liu C. Hepatitis C virus modulates human monocyte‐derived dendritic cells. Journal Of Viral Hepatitis 2009, 17: 757-769. PMID: 20051006, PMCID: PMC3731759, DOI: 10.1111/j.1365-2893.2009.01231.x.Peer-Reviewed Original ResearchConceptsHepatitis C virusNewcastle disease virusHCV infectionC virusHuman monocyte-derived dendritic cellsViral titresMonocyte-derived dendritic cellsType 1 IFN responseFlow cytometryType 1 interferon responseMaturation of DCsDendritic cell responsesReal-time reverse transcriptase-polymerase chain reactionReverse transcriptase-polymerase chain reactionTranscriptase-polymerase chain reactionHuh-7.5 cellsDC numbersDendritic cellsImmature DCsIFNβ expressionJFH-1Control monocytesHost immunityViral evasionIFN responseImmunization with host-type CD8α+ dendritic cells reduces experimental acute GVHD in an IL-10–dependent manner
Toubai T, Malter C, Tawara I, Liu C, Nieves E, Lowler K, Sun Y, Reddy P. Immunization with host-type CD8α+ dendritic cells reduces experimental acute GVHD in an IL-10–dependent manner. Blood 2009, 115: 724-735. PMID: 19965670, PMCID: PMC2810989, DOI: 10.1182/blood-2009-06-229708.Peer-Reviewed Original ResearchConceptsT cell responsesDendritic cellsT cellsImmune responseUndesirable immune responsesIL-10Major histocompatibilityDonor T-cell responsesIL-10-dependent mannerExperimental acute GVHDImmunization of donorsDonor T cellsAntigen-specific mannerB6 modelBALB/c T cellsCertain immune responsesBALB/cAcute GVHDHost diseaseInterleukin-10Active immunizationInflammatory cytokinesVaccination strategiesAntigen specificityGVHDT helper17 Cells Are Sufficient But Not Necessary to Induce Acute Graft-Versus-Host Disease
Iclozan C, Yu Y, Liu C, Liang Y, Yi T, Anasetti C, Yu X. T helper17 Cells Are Sufficient But Not Necessary to Induce Acute Graft-Versus-Host Disease. Transplantation And Cellular Therapy 2009, 16: 170-178. PMID: 19804837, PMCID: PMC3876952, DOI: 10.1016/j.bbmt.2009.09.023.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodies, MonoclonalBody WeightBone Marrow TransplantationCD3 ComplexCells, CulturedGraft vs Host DiseaseGraft vs Host ReactionInterferon-gammaInterleukin-17MiceMice, Inbred StrainsMice, TransgenicNuclear Receptor Subfamily 1, Group F, Member 3Severity of Illness IndexSurvival AnalysisTh1 CellsTime FactorsT-Lymphocyte SubsetsT-Lymphocytes, Helper-InducerTumor Necrosis Factor-alphaWhole Body ImagingConceptsHost diseaseBALB/c recipientsBone marrow transplantation settingAcute Graft-VersusT helper17 cellsGVHD target organsInduction of graftPathogenesis of GVHDCD4 T cellsGraft-VersusGVHD developmentC recipientsT helperTh17 cellsAllogeneic recipientsAutoimmune diseasesC57BL/6 miceSyngeneic recipientsTransplantation settingGVHDT cellsIFN-gammaTarget organsSuperior expansionDiseasePrimary Human Hepatocyte Culture for HCV Study
Zhu H, Elyar J, Foss R, Hemming A, Hall E, LeCluyse E, Liu C. Primary Human Hepatocyte Culture for HCV Study. Methods In Molecular Biology 2009, 510: 373-382. PMID: 19009276, DOI: 10.1007/978-1-59745-394-3_28.Peer-Reviewed Original ResearchConceptsHuman primary hepatocytesAntiviral researchPrimary hepatocyte culturesHuman primary hepatocyte culturesSmall animal modelsPrimary hepatocytesHepatocyte culturesPrimary human hepatocyte culturesHCV infectionHCV pathogenesisLiver perfusionHuman hepatocyte culturesHCV researchHost-virus interactionsInfectious virusHCV studiesPrimary culturesCell model systemHepatocyte isolationHepatocytesJFH1PathogenesisPerfusionInfection
2004
Blockade of CXCR3 Receptor:Ligand Interactions Reduces Leukocyte Recruitment to the Lung and the Severity of Experimental Idiopathic Pneumonia Syndrome
Hildebrandt G, Corrion L, Olkiewicz K, Lu B, Lowler K, Duffner U, Moore B, Kuziel W, Liu C, Cooke K. Blockade of CXCR3 Receptor:Ligand Interactions Reduces Leukocyte Recruitment to the Lung and the Severity of Experimental Idiopathic Pneumonia Syndrome. The Journal Of Immunology 2004, 173: 2050-2059. PMID: 15265940, DOI: 10.4049/jimmunol.173.3.2050.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBone Marrow CellsBronchoalveolar Lavage FluidCells, CulturedChemokine CXCL10Chemokine CXCL9Chemokines, CXCChemotaxis, LeukocyteCrosses, GeneticFemaleHematopoietic Stem Cell TransplantationInterferon-gammaLigandsLungLymphocyte ActivationMiceMice, Inbred C57BLMice, KnockoutPneumoniaReceptors, CCR5Receptors, ChemokineReceptors, CXCR3SpleenT-Lymphocytes, CytotoxicTransplantation, HomologousTumor Necrosis Factor-alphaConceptsIdiopathic pneumonia syndromeDonor T cellsPneumonia syndromeIP-10TNF-alphaT cellsIFN-gammaCXCR3 receptorDevelopment of IPSExperimental Idiopathic Pneumonia SyndromeIP-10 protein levelsAllogeneic stem cell transplantationAllo-SCT recipientsInfiltration of IFNStandard immunosuppressive therapyT cell subsetsBronchoalveolar lavage fluidStem cell transplantationT cell recruitmentControl-treated animalsImmunosuppressive therapyLigand MigAllo-SCTFatal complicationLung injuryDonor-derived TNF-α regulates pulmonary chemokine expression and the development of idiopathic pneumonia syndrome after allogeneic bone marrow transplantation
Hildebrandt G, Olkiewicz K, Corrion L, Chang Y, Clouthier S, Liu C, Cooke K. Donor-derived TNF-α regulates pulmonary chemokine expression and the development of idiopathic pneumonia syndrome after allogeneic bone marrow transplantation. Blood 2004, 104: 586-593. PMID: 15069018, DOI: 10.1182/blood-2003-12-4259.Peer-Reviewed Original ResearchConceptsDevelopment of IPSIdiopathic pneumonia syndromeAllogeneic bone marrow transplantationBone marrow transplantationTNF-alphaT cell-derived TNF-alphaPneumonia syndromeMarrow transplantationT cellsBronchoalveolar lavage fluid levelsTNF-alpha-deficient miceAllo-BMT recipientsPulmonary chemokine expressionLavage fluid levelsSignificant lung injuryInflammatory chemokine productionTNF-alpha secretionSignificant effector moleculesAllo-BMTBMT recipientsLung injuryChemokine expressionChemokine productionSyngeneic controlsTumor necrosis
2003
Long‐term interleukin 10 therapy in chronic hepatitis C patients has a proviral and anti‐inflammatory effect
Nelson D, Tu Z, Soldevila‐Pico C, Abdelmalek M, Zhu H, Xu Y, Cabrera R, Liu C, Davis G. Long‐term interleukin 10 therapy in chronic hepatitis C patients has a proviral and anti‐inflammatory effect. Hepatology 2003, 38: 859-868. PMID: 14512873, DOI: 10.1002/hep.1840380412.Peer-Reviewed Original ResearchConceptsIL-10HCV RNASerum ALTCytokine productionChronic hepatitis C patientsFunctional T cell analysesSerum HCV RNA levelsHCV-specific CD4Hepatic inflammation scoresIL-10 administrationInterleukin-10 therapyPBMC cytokine productionTh2 dominant profileHCV RNA levelsHepatitis C patientsT cell analysisTh2 cytokine productionAnti-inflammatory effectsAcute flaresC patientsDisease activityMeq/mLViral burdenAdvanced fibrosisLiver biopsyImpaired thymic negative selection causes autoimmune graft-versus-host disease
Teshima T, Reddy P, Liu C, Williams D, Cooke K, Ferrara J. Impaired thymic negative selection causes autoimmune graft-versus-host disease. Blood 2003, 102: 429-435. PMID: 12663438, DOI: 10.1182/blood-2003-01-0266.Peer-Reviewed Original ResearchMeSH KeywordsAdoptive TransferAnimalsAutoimmune DiseasesBone Marrow TransplantationCD4-Positive T-LymphocytesCD8-Positive T-LymphocytesCells, CulturedClonal DeletionDendritic CellsEpithelial CellsFemaleGraft vs Host DiseaseInflammation MediatorsInterleukin-1MiceMice, Inbred C57BLRadiation ChimeraRadiation ToleranceSelf ToleranceSkinSpleenThymectomyThymus GlandT-Lymphocyte SubsetsTumor Necrosis Factor-alphaVisceraConceptsThymic negative selectionSystemic autoimmune diseaseAntigen-presenting cellsAutoimmune diseasesHost diseaseT cellsAutoreactive T cell repertoireClass IIThymic antigen-presenting cellsMajor histocompatibility complex class IIHistocompatibility complex class IIImpaired negative selectionLethal autoimmune diseaseBone marrow chimerasPeripheral regulatory mechanismsT cell repertoireMHC class IIMHC class INegative selectionAcute graftAcute GVHDAutoimmune graftPeripheral CD4Adoptive transferNaive mice
2002
Enhanced allostimulatory activity of host antigen-presenting cells in old mice intensifies acute graft-versus-host disease
Ordemann R, Hutchinson R, Friedman J, Burakoff S, Reddy P, Duffner U, Braun T, Liu C, Teshima T, Ferrara J. Enhanced allostimulatory activity of host antigen-presenting cells in old mice intensifies acute graft-versus-host disease. Journal Of Clinical Investigation 2002, 109: 1249-1256. PMID: 11994414, PMCID: PMC150964, DOI: 10.1172/jci14793.Peer-Reviewed Original ResearchMeSH KeywordsAgingAnimalsAntigen-Presenting CellsBone Marrow TransplantationCells, CulturedDisease Models, AnimalFemaleGraft vs Host DiseaseHumansIntestine, SmallKiller Cells, NaturalLipopolysaccharidesLymphocyte ActivationMiceMice, Inbred C57BLSurvival RateT-Lymphocyte SubsetsTransplantation ChimeraTransplantation, HomologousTumor Necrosis Factor-alphaConceptsAntigen-presenting cellsDonor T-cell responsesHost antigen-presenting cellsT cell responsesOld miceAcute graftAllogeneic BMTHost diseaseOlder recipientsBM chimerasBMT modelT cellsCell responsesBone marrow transplantation recipientsDonor T cellsMore TNF-alphaMarrow transplantation recipientsMurine BMT modelHitherto unsuspected mechanismAcute GVHDAllostimulatory activityGVHD mortalitySevere GVHDWorse GVHDYoung BM
1994
Identification of factor-binding sites in the duck hepatitis B virus enhancer and in vivo effects of enhancer mutations.
Liu C, Mason W, Burch J. Identification of factor-binding sites in the duck hepatitis B virus enhancer and in vivo effects of enhancer mutations. Journal Of Virology 1994, 68: 2286-96. PMID: 8139013, PMCID: PMC236704, DOI: 10.1128/jvi.68.4.2286-2296.1994.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceCell NucleusCells, CulturedDNA Mutational AnalysisDNA, ViralDNA-Binding ProteinsDucksEnhancer Elements, GeneticHepatitis B Virus, DuckHepatocyte Nuclear Factor 1Hepatocyte Nuclear Factor 1-betaLiverMolecular Sequence DataMutagenesis, Site-DirectedNuclear ProteinsPancreasProtein BindingTranscription FactorsConceptsHepatitis B virus enhancerVirus replicationDuck hepatitis B virus replicationHepatitis B virus replicationB virus replicationHepatitis B virusCore gene promoterLMH chicken hepatoma cellsHuman growth hormoneB virusLiver-enriched transcription factorsTwo- to fourfoldVivo effectsIntact viral genomeGrowth hormoneVirus enhancerPrimary hepatocyte culturesShort-term assaysProductive infectionSignificant inhibitionVirus RNA synthesisVirusCell linesHepatoma cellsFactor-binding sitesCharacterization of the antiviral effects of 2′ carbodeoxyguanosine in ducks chronically infected with duck hepatitis B virus
Mason W, Cullen J, Saputelli J, Wu T, Liu C, London W, Lustbader E, Schaffer P, O'Connell A, Fourel I, Aldrich C, Jilbert A. Characterization of the antiviral effects of 2′ carbodeoxyguanosine in ducks chronically infected with duck hepatitis B virus. Hepatology 1994, 19: 398-411. PMID: 8294097, DOI: 10.1002/hep.1840190219.Peer-Reviewed Original ResearchConceptsHepatitis B virusDuck hepatitis B virusB virusInfected hepatocytesHigh doseHepatitis B virus infectionModerate liver injuryB virus infectionLong-term therapyAntiviral immune responseNucleoside analogue inhibitorsOvo infectionLiver injuryAntiviral therapyResidual virusChronic infectionAntiviral effectVirus infectionDrug treatmentImmune responseHistological evaluationMicrograms/Virus replicationTherapyInfection
1991
Chronic hepadnavirus infections.
Mason W, Summers J, Liu C, Condreay L, Wu T. Chronic hepadnavirus infections. Progress In Clinical And Biological Research 1991, 364: 235-42. PMID: 2020700.Peer-Reviewed Original Research