2011
Development of optimized AAV3 serotype vectors: mechanism of high-efficiency transduction of human liver cancer cells
Cheng B, Ling C, Dai Y, Lu Y, Glushakova L, Gee S, McGoogan K, Aslanidi G, Park M, Stacpoole P, Siemann D, Liu C, Srivastava A, Ling C. Development of optimized AAV3 serotype vectors: mechanism of high-efficiency transduction of human liver cancer cells. Gene Therapy 2011, 19: 375-384. PMID: 21776025, PMCID: PMC3519243, DOI: 10.1038/gt.2011.105.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBreast NeoplasmsCarcinoma, HepatocellularCell Line, TumorDependovirusFemaleGenetic VectorsHumansLiver NeoplasmsMiceMutagenesis, Site-DirectedProteasome InhibitorsProtein Structure, TertiaryProto-Oncogene Proteins c-metTransduction, GeneticTransplantation, HeterologousTyrosineVirus IntegrationConceptsHigh-efficiency transductionAAV3 vectorsTransduction efficiencySerotype vectorsSurface-exposed tyrosinePotential gene therapyHuman hepatocyte growth factor receptorHuman liver cancer cellsGene therapyLiver cancer cellsTransgene expressionVector entryAAV3 capsidsDirect intratumoral injectionAAV3Tail vein injectionLiver tumor cellsIntratumoral injectionCancer cellsEfficiencyAdenoVectorIntracellular kinase domainVirus serotypes
2009
AAV3-mediated transfer and expression of the pyruvate dehydrogenase E1 alpha subunit gene causes metabolic remodeling and apoptosis of human liver cancer cells
Glushakova L, Lisankie M, Eruslanov E, Ojano-Dirain C, Zolotukhin I, Liu C, Srivastava A, Stacpoole P. AAV3-mediated transfer and expression of the pyruvate dehydrogenase E1 alpha subunit gene causes metabolic remodeling and apoptosis of human liver cancer cells. Molecular Genetics And Metabolism 2009, 98: 289-299. PMID: 19586787, PMCID: PMC2779054, DOI: 10.1016/j.ymgme.2009.05.010.Peer-Reviewed Original ResearchConceptsPyruvate dehydrogenase complexSubunit geneReporter geneWarburg effectCell deathCancer cellsGreen fluorescent reporter geneLiver cancer cellsAlpha-subunit geneFluorescent reporter geneHuman hepatoblastomaE1alpha subunit geneHuH-6 cellsApoptotic cell deathProtein expressionMitochondrial oxidative metabolismHuman alpha-fetoprotein promoterMitochondrial respiratory chain activityBeta-actin promoterRespiratory chain activityChicken beta-actin promoterReactive oxygen speciesExpression systemDehydrogenase complexMetabolic remodeling
2005
Impact of Humoral Immune Response on Distribution and Efficacy of Recombinant Adeno-Associated Virus-Derived Acid -Glucosidase in a Model of Glycogen Storage Disease Type II
Cresawn K, Fraites T, Wasserfall C, Atkinson M, Lewis M, Porvasnik S, Liu C, Mah C, Byrne B. Impact of Humoral Immune Response on Distribution and Efficacy of Recombinant Adeno-Associated Virus-Derived Acid -Glucosidase in a Model of Glycogen Storage Disease Type II. Human Gene Therapy 2005, 16: 68-80. PMID: 15703490, DOI: 10.1089/hum.2005.16.68.Peer-Reviewed Original ResearchConceptsGlycogen storage disease type IIImmune-tolerant miceStorage disease type IIAntibody responseContractile functionImmune responseHumoral immune responseHind limb musclesAcid alpha-glucosidaseCardiorespiratory failureType IIAntibody formationReduced glycogenGlycogen reductionLysosomal storage diseaseSoleus muscleGAA levelsMiceEnzyme levelsSkeletal muscleStorage diseaseLevel of activityMuscleRecombinant adenoAlpha-glucosidase