2022
The Enthesopathy of XLH Is a Mechanical Adaptation to Osteomalacia: Biomechanical Evidence from Hyp Mice
Macica CM, Luo J, Tommasini SM. The Enthesopathy of XLH Is a Mechanical Adaptation to Osteomalacia: Biomechanical Evidence from Hyp Mice. Calcified Tissue International 2022, 111: 313-322. PMID: 35618776, DOI: 10.1007/s00223-022-00989-7.Peer-Reviewed Original ResearchConceptsUltimate strengthLoading conditionsMaximum strainNormal loading conditionsTensile testsMechanical propertiesTensile stiffnessWT miceHyp miceMechanical loadingMechanical stressBiomechanical testingSoft boneStiffnessBone matrixAlkaline phosphataseMajor comorbiditiesAchilles insertionClinical picturePhysical functionMechanical adaptationHyperplastic expansionMurine modelTriceps suraeStrength
2016
Novel anatomic adaptation of cortical bone to meet increased mineral demands of reproduction
Macica CM, King HE, Wang M, McEachon CL, Skinner CW, Tommasini SM. Novel anatomic adaptation of cortical bone to meet increased mineral demands of reproduction. Bone 2016, 85: 59-69. PMID: 26825813, PMCID: PMC7429445, DOI: 10.1016/j.bone.2015.12.056.Peer-Reviewed Original ResearchConceptsHyp miceMineral homeostasisElevated serum PTHMaternal bone massMineralized trabecular boneIntestinal calcium absorptionCortical boneWild-type miceSerum PTHCalcitriol productionFemale patientsMaternal skeletonPostnatal nutritionCalcium absorptionBone resorptionFracture riskMaternal adaptationBone massBone fragilityMurine modelIntracortical porosityUnaffected miceMouse modelMMP-13Fetal development
2012
Mineralizing Enthesopathy Is a Common Feature of Renal Phosphate-Wasting Disorders Attributed to FGF23 and Is Exacerbated by Standard Therapy in Hyp Mice
Karaplis A, Bai X, Falet J, Macica C. Mineralizing Enthesopathy Is a Common Feature of Renal Phosphate-Wasting Disorders Attributed to FGF23 and Is Exacerbated by Standard Therapy in Hyp Mice. Endocrinology 2012, 153: 5906-5917. PMID: 23038738, PMCID: PMC3512070, DOI: 10.1210/en.2012-1551.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsExtracellular Matrix ProteinsFamilial Hypophosphatemic RicketsFemaleFibroblast Growth Factor-23Fibroblast Growth FactorsGenetic Diseases, X-LinkedImmunohistochemistryKidneyMaleMiceMice, Inbred C57BLMice, TransgenicModels, GeneticMutationPedigreePhosphatesPhosphoproteinsRheumatic DiseasesTransgenesUp-RegulationConceptsPhosphate-wasting disordersStandard therapyFGF23 levelsElevated fibroblast growth factor 23Hyp miceRenal phosphate-wasting disordersFibroblast growth factor 23High FGF23 levelsGrowth factor 23Elevated FGF23 levelsAction of FGF23Dentin matrix acidic phosphoprotein 1Phosphate wasting disorderAutosomal recessive hypophosphatemic ricketsRecessive hypophosphatemic ricketsLigament insertion sitesChildhood managementOral phosphateFactor 23Achilles insertionDisease morbidityPotential morbidityBone spursUntoward effectsMurine model
2011
Genetic evidence of the regulatory role of parathyroid hormone–related protein in articular chondrocyte maintenance in an experimental mouse model
Macica C, Liang G, Nasiri A, Broadus AE. Genetic evidence of the regulatory role of parathyroid hormone–related protein in articular chondrocyte maintenance in an experimental mouse model. Arthritis & Rheumatism 2011, 63: 3333-3343. PMID: 21702022, PMCID: PMC3197958, DOI: 10.1002/art.30515.Peer-Reviewed Original ResearchConceptsHormone-related proteinDegenerative changesKO miceParathyroid hormone-related proteinMale KO miceExperimental mouse modelMouse articular cartilageTotal histologic scoreIndian hedgehogTibial articular surfaceArticular chondrocytesArticular cartilageGrowth differentiation factor 5Degenerative findingsControl miceHistologic scoresMouse modelCompensatory increaseTime-course studyDifferentiation factor 5PTHrPRegulatory roleMiceConditional deletionChondrocyte maintenanceAn Atypical Degenerative Osteoarthropathy in Hyp Mice is Characterized by a Loss in the Mineralized Zone of Articular Cartilage
Liang G, VanHouten J, Macica C. An Atypical Degenerative Osteoarthropathy in Hyp Mice is Characterized by a Loss in the Mineralized Zone of Articular Cartilage. Calcified Tissue International 2011, 89: 151-162. PMID: 21643724, DOI: 10.1007/s00223-011-9502-4.Peer-Reviewed Original Research
2009
Survey of the Enthesopathy of X-Linked Hypophosphatemia and Its Characterization in Hyp Mice
Liang G, Katz LD, Insogna KL, Carpenter TO, Macica CM. Survey of the Enthesopathy of X-Linked Hypophosphatemia and Its Characterization in Hyp Mice. Calcified Tissue International 2009, 85: 235-246. PMID: 19609735, PMCID: PMC2988401, DOI: 10.1007/s00223-009-9270-6.Peer-Reviewed Original ResearchMeSH KeywordsAchilles TendonAdolescentAdultAgedAnimalsBiomarkersCalcinosisChildDisease Models, AnimalDisease ProgressionFamilial Hypophosphatemic RicketsFemaleFibroblast Growth Factor-23Fibroblast Growth FactorsGenetic Diseases, X-LinkedHumansMiceMice, Inbred C57BLMiddle AgedPatellar LigamentPhenotypeQuadriceps MuscleRadiographyRheumatic DiseasesTendinopathyTendonsYoung AdultConceptsFGF-23Fibroblast growth factor receptor 3Hyp miceMajority of patientsHigh circulating levelsPhosphate-regulating hormoneBone spur formationTendon insertion siteGrowth factor receptor 3Insertion siteLigament insertion sitesCirculating LevelsPhosphate excretionBone-forming osteoblastsHeterotopic calcificationOsteophyte formationHistological examinationMurine modelReceptor 3Spur formationHypophosphatemiaEnthesis fibrocartilageBone mineralizationBiochemical milieuMice
2008
Regulation of articular chondrocyte proliferation and differentiation by indian hedgehog and parathyroid hormone–related protein in mice
Chen X, Macica CM, Nasiri A, Broadus AE. Regulation of articular chondrocyte proliferation and differentiation by indian hedgehog and parathyroid hormone–related protein in mice. Arthritis & Rheumatism 2008, 58: 3788-3797. PMID: 19035497, PMCID: PMC2599803, DOI: 10.1002/art.23985.Peer-Reviewed Original ResearchConceptsPTHrP expressionParathyroid hormone-related proteinArticular cartilagePTHrP-knockout micePathogenesis of arthritisWeight-bearing jointsHormone-related proteinChondrocyte proliferationIndian hedgehogGrowth plate structureCell of originArticular chondrocytesEpiphyseal growth zoneSecondary ossification centerArticular chondrocyte differentiationArticular chondrocyte proliferationAdult miceAxis participatesKnockin miceMicePTHrPProtein axisOssification centersIhhCartilage
2007
The PTHrP Functional Domain Is at the Gates of Endochondral Bones
BROADUS AE, MACICA C, CHEN X. The PTHrP Functional Domain Is at the Gates of Endochondral Bones. Annals Of The New York Academy Of Sciences 2007, 1116: 65-81. PMID: 18083922, DOI: 10.1196/annals.1402.061.Peer-Reviewed Original ResearchConceptsGene expression productsEndochondral boneGene regulation experimentsFunctional domainsBone cell populationsMesenchymal condensationLow abundanceChondrocyte populationsKnockin miceCell populationsGrowth plate cartilageUnrecognized siteInsertion siteExpressionEpiphyseal cartilageSitesLacZAbundanceMechanical regulation of PTHrP expression in entheses
Chen X, Macica C, Nasiri A, Judex S, Broadus AE. Mechanical regulation of PTHrP expression in entheses. Bone 2007, 41: 752-759. PMID: 17869201, PMCID: PMC2219696, DOI: 10.1016/j.bone.2007.07.020.Peer-Reviewed Original ResearchConceptsMedial collateral ligamentPTHrP expressionSurgical transectionBone cell populationsTail suspensionType 1 PTH/PTHrP receptorCell populationsPTH/PTHrP receptorDetailed histological evaluationSitu hybridization histochemical techniquesDistinct temporospatial patternLigament insertion sitesMetaphyseal periosteumPTHrP receptorOsteoclastic activityCollateral ligamentHistological evaluationReporter miceKnockin miceInsertion siteKnockin reporter micePTHrPPTHrP geneCD-1PeriosteumIGF‐1 stimulates de novo fatty acid biosynthesis by Schwann cells during myelination
Liang G, Cline GW, Macica CM. IGF‐1 stimulates de novo fatty acid biosynthesis by Schwann cells during myelination. Glia 2007, 55: 632-641. PMID: 17299765, DOI: 10.1002/glia.20496.Peer-Reviewed Original ResearchMeSH KeywordsAcetatesAnimalsCell CommunicationCells, CulturedCoculture TechniquesFatty AcidsGanglia, SpinalInsulin-Like Growth Factor IMembrane LipidsMyelin SheathNeurons, AfferentPeripheral Nervous SystemPhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktRatsRats, Sprague-DawleySchwann CellsSignal TransductionConceptsInsulin-like growth factor-1Fatty acid synthesizing enzymesSynthesizing enzymesIGF-1 treatmentMyelin membrane formationGrowth factor-1PI3K/Akt pathwaySchwann cell differentiationPI3K/AktWestern blot analysisSciatic nerveIGF-1Schwann cellsElectrospray mass spectroscopy analysisCell coculturesMyelin-specific proteinsGrowth factorAkt pathwayMyelinationFactor 1Fatty acid synthesisCocultureBlot analysisEarly eventsMyelin membrane
2006
Induction of parathyroid hormone‐related peptide following peripheral nerve injury: Role as a modulator of Schwann cell phenotype
Macica CM, Liang G, Lankford KL, Broadus AE. Induction of parathyroid hormone‐related peptide following peripheral nerve injury: Role as a modulator of Schwann cell phenotype. Glia 2006, 53: 637-648. PMID: 16470617, DOI: 10.1002/glia.20319.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, NewbornCell DifferentiationCell ProliferationCells, CulturedCyclic AMP Response Element-Binding ProteinDisease Models, AnimalGanglia, SpinalGrowth ConesLigationMiceNerve RegenerationNeurons, AfferentParathyroid Hormone-Related ProteinPeripheral Nerve InjuriesPeripheral NervesRatsRats, Sprague-DawleyReceptor, Parathyroid Hormone, Type 1RNA, MessengerSchwann CellsSciatic NerveSciatic NeuropathyUp-RegulationConceptsSciatic nerve injury modelNerve injury modelPeripheral nerve injuryRole of PTHrPHormone-related peptideSchwann cellsSciatic nerveNerve injuryInjury modelNerve regenerationNervous systemParathyroid hormone-related peptideInjured sciatic nerveDedifferentiated Schwann cellsSchwann cell phenotypeRat nervous systemPeripheral nervous systemPTHrP receptor mRNASuccessful nerve regenerationType 1 collagen matrixSchwann cell culturesSympathetic gangliaPTHrP functionsPTHrP mRNAReceptor mRNAInitial Characterization of PTH‐Related Protein Gene‐Driven lacZ Expression in the Mouse*
Chen X, Macica CM, Dreyer BE, Hammond VE, Hens JR, Philbrick WM, Broadus AE. Initial Characterization of PTH‐Related Protein Gene‐Driven lacZ Expression in the Mouse*. Journal Of Bone And Mineral Research 2006, 21: 113-123. PMID: 16355280, DOI: 10.1359/jbmr.051005.Peer-Reviewed Original Research
2005
Stretch‐Induced PTH‐Related Protein Gene Expression in Osteoblasts*
Chen X, Macica CM, Ng KW, Broadus AE. Stretch‐Induced PTH‐Related Protein Gene Expression in Osteoblasts*. Journal Of Bone And Mineral Research 2005, 20: 1454-1461. PMID: 16007342, DOI: 10.1359/jbmr.2005.20.8.1454.Peer-Reviewed Original ResearchConceptsAnabolic effectsStretch-activated potassium channelsCandidate mediatorsCyclical stretchPotassium channelsL-type calcium channelsN-terminal PTHPTHrP mRNA expressionStretch-activated cation channelsSmooth muscle cellsOsteoblast-like cellsUMR 201PTHrP gene expressionHypotonic solutionEndogenous mediatorsPTHrP mRNAIntracellular acidosisExtracellular calciumCalcium channelsFlexercell apparatusPTHrPRNase protection assaysMRNA expressionMuscle cellsSkeletal mass
2003
PTHrP regulates cerebral blood flow and is neuroprotective
Macica CM, Broadus AE. PTHrP regulates cerebral blood flow and is neuroprotective. AJP Regulatory Integrative And Comparative Physiology 2003, 284: r1019-r1020. PMID: 12626363, DOI: 10.1152/ajpregu.00001.2003.Peer-Reviewed Original ResearchModulation of the Kv3.1b Potassium Channel Isoform Adjusts the Fidelity of the Firing Pattern of Auditory Neurons
Macica CM, von Hehn CA, Wang LY, Ho CS, Yokoyama S, Joho RH, Kaczmarek LK. Modulation of the Kv3.1b Potassium Channel Isoform Adjusts the Fidelity of the Firing Pattern of Auditory Neurons. Journal Of Neuroscience 2003, 23: 1133-1141. PMID: 12598601, PMCID: PMC6742259, DOI: 10.1523/jneurosci.23-04-01133.2003.Peer-Reviewed Original ResearchMeSH KeywordsAction PotentialsAnimalsBrain StemCells, CulturedCHO CellsCricetinaeElectric ConductivityEvoked Potentials, AuditoryKineticsMiceMice, KnockoutNeuronsNeuropeptidesPatch-Clamp TechniquesPhosphorylationPotassium ChannelsPotassium Channels, Voltage-GatedProtein IsoformsProtein Kinase CSerineShaw Potassium ChannelsTetradecanoylphorbol AcetateConceptsTrapezoid bodyMedial nucleusAuditory neuronsHigh-frequency stimulationWild-type neuronsKv3.1 potassium channelHigh-threshold componentPotassium channel isoformsGreat temporal precisionPartial decreaseProtein kinase C activationAction potentialsLocation of soundsMice resultsFiring patternsNeuronsSensory stimulationPotassium channelsChannel isoformsKinase C activationKv3.1Kv3.1 geneStimulationHigh frequencyProtein kinase C
2001
Casein Kinase 2 Determines the Voltage Dependence of the Kv3.1 Channel in Auditory Neurons and Transfected Cells
Macica C, Kaczmarek L. Casein Kinase 2 Determines the Voltage Dependence of the Kv3.1 Channel in Auditory Neurons and Transfected Cells. Journal Of Neuroscience 2001, 21: 1160-1168. PMID: 11160386, PMCID: PMC6762230, DOI: 10.1523/jneurosci.21-04-01160.2001.Peer-Reviewed Original ResearchMeSH KeywordsAlkaline PhosphataseAnimalsAuditory PathwaysBinding SitesBrain StemCasein Kinase IICDC2-CDC28 KinasesCHO CellsCricetinaeCyclin-Dependent Kinase 2Cyclin-Dependent KinasesElectric StimulationEnzyme InhibitorsIn Vitro TechniquesMembrane PotentialsNeuronsNeuropeptidesPatch-Clamp TechniquesPhosphorylationPotassium ChannelsPotassium Channels, Voltage-GatedPrecipitin TestsProtein Kinase CProtein Serine-Threonine KinasesRatsShaw Potassium ChannelsTetradecanoylphorbol AcetateTransfectionConceptsCasein kinase 2Kinase 2Casein kinase IIProtein kinase CKv3.1 channelsChinese hamster ovary cellsHamster ovary cellsConstitutive phosphorylationPhosphatase treatmentKinase IIKinase CTransfected CellsVoltage-dependent activationOvary cellsWhole-cell conductancePhosphorylationPotassium channelsRectifier channelsBiophysical characteristicsInactivationKv3.1 potassium channelVoltage dependenceActivationKv3.1Patch-clamp recordings
1998
Role of the NH2 terminus of the cloned renal K+ channel, ROMK1, in arachidonic acid-mediated inhibition
Macica C, Yang Y, Lerea K, Hebert S, Wang W. Role of the NH2 terminus of the cloned renal K+ channel, ROMK1, in arachidonic acid-mediated inhibition. American Journal Of Physiology 1998, 274: f175-f181. PMID: 9458837, DOI: 10.1152/ajprenal.1998.274.1.f175.Peer-Reviewed Original ResearchAlanineAmino Acid SequenceAmino Acid SubstitutionAnimalsArachidonic AcidCell LineCloning, MolecularFemaleIon Channel GatingMembrane PotentialsMolecular Sequence DataMutagenesis, Site-DirectedOocytesPotassium ChannelsPotassium Channels, Inwardly RectifyingProtein Kinase CRatsRecombinant ProteinsSequence AlignmentSerineTransfectionXenopus laevis
1996
Arachidonic acid inhibits activity of cloned renal K+ channel, ROMK1
Macica CM, Yang Y, Hebert SC, Wang WH. Arachidonic acid inhibits activity of cloned renal K+ channel, ROMK1. American Journal Of Physiology 1996, 271: f588-f594. PMID: 8853420, DOI: 10.1152/ajprenal.1996.271.3.f588.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsArachidonic AcidCaffeic AcidsCloning, MolecularCyclooxygenase InhibitorsCytochrome P-450 Enzyme InhibitorsFatty AcidsFatty Acids, UnsaturatedFemaleIndomethacinKidney MedullaKineticsLipoxygenase InhibitorsOocytesPatch-Clamp TechniquesPotassium Channel BlockersPotassium ChannelsRatsXenopus laevis
1994
TNF production by the medullary thick ascending limb of Henle's loop
Macica C, Escalante B, Conners M, Ferreri N. TNF production by the medullary thick ascending limb of Henle's loop. Kidney International 1994, 46: 113-121. PMID: 7933828, DOI: 10.1038/ki.1994.250.Peer-Reviewed Original Research
1993
Characterization of cytochrome P-450-dependent arachidonic acid metabolism in rabbit intestine
Macica C, Balazy M, Falck J, Mioskowski C, Carroll M. Characterization of cytochrome P-450-dependent arachidonic acid metabolism in rabbit intestine. American Journal Of Physiology 1993, 265: g735-g741. PMID: 8238357, DOI: 10.1152/ajpgi.1993.265.4.g735.Peer-Reviewed Original ResearchConceptsEpithelial ion transportAA metabolitesNew Zealand white male rabbitsRegional blood flowArachidonic acid metabolismWhite male rabbitsDuodenal microsomesIleal microsomesMesenteric bedIntestinal functionArachidonate metabolismBW 755CBlood flowHigh-pressure liquid chromatographyEnzyme inhibitorsMale rabbitsAA metabolismIntestinal tractSKF-525ALipoxygenase inhibitorsRabbit intestineMicrosomal activityPresence of NADPHAbsence of NADPHAcid metabolism