2015
Noncanonical Sites of Adult Neurogenesis in the Mammalian Brain
Feliciano DM, Bordey A, Bonfanti L. Noncanonical Sites of Adult Neurogenesis in the Mammalian Brain. Cold Spring Harbor Perspectives In Biology 2015, 7: a018846. PMID: 26384869, PMCID: PMC4588058, DOI: 10.1101/cshperspect.a018846.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus Statements
2013
Neonatal subventricular zone electroporation.
Feliciano DM, Lafourcade CA, Bordey A. Neonatal subventricular zone electroporation. Journal Of Visualized Experiments 2013 PMID: 23426329, PMCID: PMC3601042, DOI: 10.3791/50197.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsNeural stem cellsGenetic engineeringEmbryonic neural stem cellsWhole animal levelMultiple cell typesSVZ neural stem cellsMammalian systemsMolecular pathwaysCell typesStem cellsTime-effective alternativeRodent forebrainAnimal levelElectroporationEpendymal cellsInvertebratesCellsCortical developmentRobust labelingProgenyCentral nervous system disordersNervous system disordersDifferentiationPathwayVast majority
2012
Preparation of acute subventricular zone slices for calcium imaging.
Lacar B, Young SZ, Platel JC, Bordey A. Preparation of acute subventricular zone slices for calcium imaging. Journal Of Visualized Experiments 2012, e4071. PMID: 23023088, PMCID: PMC3490239, DOI: 10.3791/4071.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsMeSH KeywordsAniline CompoundsAnimalsAstrocytesCalciumFluorescent DyesLateral VentriclesMiceMicroscopy, ConfocalMicrotomyXanthenesConceptsCell typesSVZ cellsProgenitor cellsNeurogenic zonesSubventricular zoneFluo-4 AM dyeNeural progenitor cellsIntermediate progenitor cellsExtracellular signalsDiffusible signalsIntercellular signalingSVZ cell typesTime-lapse moviesCalcium indicator dyeCalcium activityIndividual cellsRostral-caudal axisNeuroblast migrationThree-dimensional arrangementAdult hippocampal subgranular zonePostnatal brainAdhesion moleculesHippocampal subgranular zoneMature astrocytesNeuroblastsS Phase Entry of Neural Progenitor Cells Correlates with Increased Blood Flow in the Young Subventricular Zone
Lacar B, Herman P, Hartman NW, Hyder F, Bordey A. S Phase Entry of Neural Progenitor Cells Correlates with Increased Blood Flow in the Young Subventricular Zone. PLOS ONE 2012, 7: e31960. PMID: 22359646, PMCID: PMC3281100, DOI: 10.1371/journal.pone.0031960.Peer-Reviewed Original ResearchMeSH KeywordsAge FactorsAnimalsCell ProliferationHemodynamicsLateral VentriclesMiceNeural Stem CellsRegional Blood FlowS PhaseConceptsSubventricular zoneBlood flowNeural progenitor cellsAcute brain slice preparationGrowth factor injectionsProgenitor cellsReceptor agonist UIncreased blood flowBrain slice preparationSVZ cell proliferationCell proliferationLaser Doppler flowmetryLocal blood flowLocal hemodynamic responsesBasic fibroblast growth factorPostnatal subventricular zoneFibroblast growth factorAgonist UFactor injectionsVentricular injectionNeurovascular couplingAcute increaseSlice preparationDoppler flowmetryHemodynamic response
2005
Nonsynaptic GABA signaling in postnatal subventricular zone controls proliferation of GFAP-expressing progenitors
Liu X, Wang Q, Haydar TF, Bordey A. Nonsynaptic GABA signaling in postnatal subventricular zone controls proliferation of GFAP-expressing progenitors. Nature Neuroscience 2005, 8: 1179-1187. PMID: 16116450, PMCID: PMC1380263, DOI: 10.1038/nn1522.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, NewbornBotulinum ToxinsBromodeoxyuridineCadmiumCell CountCell ProliferationChelating AgentsCyclooxygenase InhibitorsDose-Response Relationship, DrugDose-Response Relationship, RadiationDrug InteractionsEgtazic AcidElectric StimulationEnzyme InhibitorsGABA AntagonistsGamma-Aminobutyric AcidGene Expression RegulationGlial Fibrillary Acidic ProteinGreen Fluorescent ProteinsImmunohistochemistryIn Vitro TechniquesLateral VentriclesMeclofenamic AcidMembrane PotentialsMiceMice, TransgenicNeuronsNickelPatch-Clamp TechniquesPotassiumSodium Channel BlockersSpider VenomsStem CellsTetrodotoxinConceptsPostnatal subventricular zoneGFAP-expressing cellsSubventricular zoneCell cycleGABAA receptorsStem cellsNeuroblastsProgenitorsGlial fibrillary acidic proteinSVZ cellsGABAA receptor currentsGABAA receptor activationFibrillary acidic proteinReceptor activationCellsProliferationGABA releaseMouse slicesLocal cuesAcidic proteinReceptor currentsSpontaneous depolarizationsGFAPGABAReceptorsAssays for measuring extracellular GABA levels and cell migration rate in acute slices
Bolteus AJ, Garganta C, Bordey A. Assays for measuring extracellular GABA levels and cell migration rate in acute slices. Brain Research 2005, 14: 126-134. PMID: 15721818, DOI: 10.1016/j.brainresprot.2004.12.005.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiological AssayCell MovementGABA Plasma Membrane Transport ProteinsGamma-Aminobutyric AcidImmunohistochemistryLateral VentriclesMass SpectrometryMembrane Transport ModulatorsMembrane Transport ProteinsMiceMicroscopy, VideoNeurochemistryNeuronsOrgan Culture TechniquesReceptors, GABAStem CellsTelencephalonConceptsRostral migratory streamSubventricular zoneEndogenous GABAGABA levelsGABA receptorsNeuronal precursorsCell migrationLocal GABA levelsExtracellular GABA levelsAcute brain slicesAdult rodent brainPostnatal subventricular zoneFuture therapeutic useNeuronal precursor migrationAcute slicesBrain slicesNeurotransmitter GABASVZ cellsOlfactory bulbRodent brainRMS explantsMigratory streamTherapeutic useNeural precursorsGABA
2003
Biophysical Properties and Ionic Signature of Neuronal Progenitors of the Postnatal Subventricular Zone In Situ
Wang DD, Krueger DD, Bordey A. Biophysical Properties and Ionic Signature of Neuronal Progenitors of the Postnatal Subventricular Zone In Situ. Journal Of Neurophysiology 2003, 90: 2291-2302. PMID: 12801891, DOI: 10.1152/jn.01116.2002.Peer-Reviewed Original ResearchConceptsRostral migratory streamSubventricular zonePassive membrane propertiesNeuronal progenitorsCell-attached patch-clamp recordingsNeuron-specific class IIIPostnatal mammalian brainPostnatal day 15Patch-clamp recordingsMigration of progenitorsPostnatal subventricular zoneHigh input resistanceCell-attached patchesBrain slicesOlfactory bulbMature neuronsStrong immunoreactivityTransient outwardDay 15KDR currentElectrophysiological propertiesAction potentialsMammalian brainMigratory streamClass III