A head-to-head comparison of ribodepletion and polyA selection approaches for Caenorhabditis elegans low input RNA-sequencing libraries
Barrett A, McWhirter R, Taylor SR, Weinreb A, Miller DM, Hammarlund M. A head-to-head comparison of ribodepletion and polyA selection approaches for Caenorhabditis elegans low input RNA-sequencing libraries. G3: Genes, Genomes, Genetics 2021, 11: jkab121. PMID: 33856427, PMCID: PMC8495925, DOI: 10.1093/g3journal/jkab121.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCaenorhabditis elegansGene Expression ProfilingPoly ARNARNA, RibosomalSequence Analysis, RNAConceptsC. elegans RNANoisy gene expressionRRNA gene sequencesRNA sequencing librariesRare cell populationsSuch challenging samplesCaenorhabditis elegansLong genesRibosomal RNAGene sequencesNoncoding RNAsCustom kitRNAseq librariesGene expressionPolyA selectionRRNA depletionLibrary preparationLibrary constructionRNAPCR duplicatesGenesRibodepletionCell populationsCell samplesExpression