2016
The influence of diisocyanate antigen preparation methodology on monoclonal and serum antibody recognition
Hagerman LM, Law BF, Bledsoe TA, Hettick JM, Kashon ML, Lemons AR, Wisnewski AV, Siegel PD. The influence of diisocyanate antigen preparation methodology on monoclonal and serum antibody recognition. Journal Of Occupational And Environmental Hygiene 2016, 13: 829-839. PMID: 27124286, PMCID: PMC5016257, DOI: 10.1080/15459624.2016.1183013.Peer-Reviewed Original ResearchConceptsOccupational asthmaDifferent conjugatesPolyclonal antibody responsePanel of antigensSpecific antibody clonesHuman serumMDI conjugationPolyclonal serum antibodiesSpecific IgEAntibody responseSerum antibodiesSerologic detectionStandard antigenMonoclonal antibodiesAntibody clonesAntibodiesAsthmaSignificant differencesAntigenSerumMAbsAntibody recognitionAntigenicityMinimal differencesConjugates
2007
Respiratory Symptoms, Sensitization, and Exposure–Response Relationships in Spray Painters Exposed to Isocyanates
Pronk A, Preller L, Raulf-Heimsoth M, Jonkers IC, Lammers JW, Wouters IM, Doekes G, Wisnewski AV, Heederik D. Respiratory Symptoms, Sensitization, and Exposure–Response Relationships in Spray Painters Exposed to Isocyanates. American Journal Of Respiratory And Critical Care Medicine 2007, 176: 1090-1097. PMID: 17656675, PMCID: PMC2176099, DOI: 10.1164/rccm.200702-215oc.Peer-Reviewed Original ResearchConceptsWork-related chest tightnessExposure-response relationshipRespiratory symptomsChest tightnessSpecific IgESpecific IgGSpecific IgE sensitizationExposure-response associationsDisease-like symptomsTime-activity informationAsthmalike symptomsIgE sensitizationIndicator of exposureSpecific sensitizationSymptomatic individualsHSA conjugatesRespiratory diseaseSpray paintersSymptomsIgESensitizationPrevalenceIgGPersonal exposureOffice workersDiisocyanate conjugate and immunoassay characteristics influence detection of specific antibodies in HDI‐exposed workers
Campo P, Wisnewski AV, Lummus Z, Cartier A, Malo J, Boulet LP, Bernstein DI. Diisocyanate conjugate and immunoassay characteristics influence detection of specific antibodies in HDI‐exposed workers. Clinical & Experimental Allergy 2007, 37: 1095-1102. PMID: 17581205, DOI: 10.1111/j.1365-2222.2007.02745.x.Peer-Reviewed Original ResearchMeSH KeywordsAdultAir Pollutants, OccupationalAsthmaBronchial Provocation TestsCyanatesEnzyme-Linked Immunosorbent AssayFemaleHumansImmunoassayImmunoglobulin EImmunoglobulin GInhalation ExposureIsocyanatesMaleMiddle AgedMolecular StructureObserver VariationOccupational DiseasesPredictive Value of TestsQuality ControlQuebecRadioallergosorbent TestReagent Kits, DiagnosticReproducibility of ResultsSensitivity and SpecificitySerum AlbuminSkin TestsConceptsSpecific inhalation challengeDiisocyanate asthmaSpecific IgEHuman serum albumin conjugatesSpecific antibodiesSpecific IgG antibodiesInhalation challengeAsthmatic reactionSpecific IgGHDI exposureIgG antibodiesTest antigenPredictive valueSerum albumin conjugatesAntigen immunoassayIgEProtein antigensIgG immunoassayShop workersInter-laboratory variabilityAlbumin conjugatesAntibodiesAsthmaRASTIgG
2004
Isocyanate vapor-induced antigenicity of human albumin
Wisnewski AV, Stowe MH, Cartier A, Liu Q, Liu J, Chen L, Redlich CA. Isocyanate vapor-induced antigenicity of human albumin. Journal Of Allergy And Clinical Immunology 2004, 113: 1178-1184. PMID: 15208602, DOI: 10.1016/j.jaci.2004.03.009.Peer-Reviewed Original ResearchConceptsHDI exposureHuman albuminAuto body shop workersHDI vaporHuman immune systemOccupational asthmaHumoral armSpecific IgEIgG titersHealthy workersImmune responseSerology studiesHuman airwaysImmune systemSignificant associationAntigenic formsShop workersAlbumin conjugatesAsthmaticsAsthmaIgEAntigenicityParallel studyAlbuminSignificant changes