2022
Glutathione reactivity with aliphatic polyisocyanates
Wisnewski AV, Liu J. Glutathione reactivity with aliphatic polyisocyanates. PLOS ONE 2022, 17: e0271471. PMID: 35839242, PMCID: PMC9286259, DOI: 10.1371/journal.pone.0271471.Peer-Reviewed Original ResearchConceptsHexamethylene diisocyanateAliphatic polyisocyanatesReaction productsMS/MSTandem MS/MSReversed-phase LC-MSPositive ion modeMajor reaction productsLiquid chromatography-coupled mass spectrometryCoupled-mass spectrometryPolymeric formulationsIon modeGlutathione reactivityFragmentation patternsGSH reactivityS linkageO groupLC-MSPolyisocyanatesIonsIsocyanate productsDiisocyanateImportant productsReactivityMS
2012
Hexamethylene diisocyanate (HDI) vapor reactivity with glutathione and subsequent transfer to human albumin
Wisnewski AV, Mhike M, Hettick JM, Liu J, Siegel PD. Hexamethylene diisocyanate (HDI) vapor reactivity with glutathione and subsequent transfer to human albumin. Toxicology In Vitro 2012, 27: 662-671. PMID: 23178851, PMCID: PMC3587365, DOI: 10.1016/j.tiv.2012.11.013.Peer-Reviewed Original ResearchConceptsHDI vaporCommon occupational allergensRespiratory tract exposureHuman albuminLevels of GSHReaction productsGSH concentrationVapor exposureIntra-molecular cyclizationClinical responseOccupational allergensAsthma pathogenesisPolyclonal rabbit serumAirway fluidSerologic assaysLower GSH concentrationsVivo reactivityRabbit serumPotential mechanismsGlutathione reactivityMass spectrometryExposureReactivityGSH solutionKey step