2023
Bacteria require phase separation for fitness in the mammalian gut
Krypotou E, Townsend G, Gao X, Tachiyama S, Liu J, Pokorzynski N, Goodman A, Groisman E. Bacteria require phase separation for fitness in the mammalian gut. Science 2023, 379: 1149-1156. PMID: 36927025, PMCID: PMC10148683, DOI: 10.1126/science.abn7229.Peer-Reviewed Original ResearchConceptsMammalian gutTranscription termination factor RhoTermination factor RhoGene regulationTranscription terminationMechanisms bacteriaBacteria interactionsHuman commensalValuable targetBacteriaRhoGut microbiotaFitnessNovel clinical applicationsTherapeutic manipulationGutHuman healthCommensalRegulation
2021
Symmetrical arrangement of proteins under release-ready vesicles in presynaptic terminals
Radhakrishnan A, Li X, Grushin K, Krishnakumar SS, Liu J, Rothman JE. Symmetrical arrangement of proteins under release-ready vesicles in presynaptic terminals. Proceedings Of The National Academy Of Sciences Of The United States Of America 2021, 118: e2024029118. PMID: 33468631, PMCID: PMC7865176, DOI: 10.1073/pnas.2024029118.Peer-Reviewed Original ResearchConceptsPlasma membraneSynaptic vesiclesSV fusionRelease-ready vesiclesFusion machinerySingle SNAREpinSV releaseExocytosis machineryMolecular eventsNative conditionsProtein componentsCultured hippocampal neuronsPriming reactionPresynaptic CaVesiclesFundamental processesProtein densityProtein massRelease of neurotransmittersNeurotransmitter releaseMachineryPresynaptic terminalsReleasable poolHippocampal neuronsVariable number
2018
A unique cytoplasmic ATPase complex defines the Legionella pneumophila type IV secretion channel
Chetrit D, Hu B, Christie PJ, Roy CR, Liu J. A unique cytoplasmic ATPase complex defines the Legionella pneumophila type IV secretion channel. Nature Microbiology 2018, 3: 678-686. PMID: 29784975, PMCID: PMC5970066, DOI: 10.1038/s41564-018-0165-z.Peer-Reviewed Original ResearchConceptsCytoplasmic complexType IV secretion channelType IV secretion systemInner membrane complexTranslocation of substratesCryo-electron tomographySecretion channelCell polesCytoplasmic ATPaseSecretion systemT4SS functionHexameric assemblyMembrane complexCytoplasmic channelsDNA complexesSubstrate transferT4SSChannel activationATPaseComplexesDistinct stagesAssemblyBiogenesisATPasesFurther analysis
2016
A novel flagellar sheath protein, FcpA, determines filament coiling, translational motility and virulence for the Leptospira spirochete
Wunder EA, Figueira CP, Benaroudj N, Hu B, Tong BA, Trajtenberg F, Liu J, Reis MG, Charon NW, Buschiazzo A, Picardeau M, Ko AI. A novel flagellar sheath protein, FcpA, determines filament coiling, translational motility and virulence for the Leptospira spirochete. Molecular Microbiology 2016, 101: 457-470. PMID: 27113476, PMCID: PMC4979076, DOI: 10.1111/mmi.13403.Peer-Reviewed Original ResearchConceptsTraverse tissue barriersHamster modelTarget organsSystemic infectionClinical isolatesLeptospira spirochetesTranslational motilityL. interrogansCell morphologyTissue barriersMotilityLeptospiraPeriplasmic flagellaSpirochetesVirulence phenotypesProtein AHelical cell morphologyInfectionDiseaseLeptospirosisPathogen L. interrogans
2012
Structural Changes in Isometrically Contracting Insect Flight Muscle Trapped following a Mechanical Perturbation
Wu S, Liu J, Reedy MC, Perz-Edwards RJ, Tregear RT, Winkler H, Franzini-Armstrong C, Sasaki H, Lucaveche C, Goldman YE, Reedy MK, Taylor KA. Structural Changes in Isometrically Contracting Insect Flight Muscle Trapped following a Mechanical Perturbation. PLOS ONE 2012, 7: e39422. PMID: 22761792, PMCID: PMC3382574, DOI: 10.1371/journal.pone.0039422.Peer-Reviewed Original Research
2011
Direct visualization of myosin-binding protein C bridging myosin and actin filaments in intact muscle
Luther PK, Winkler H, Taylor K, Zoghbi ME, Craig R, Padrón R, Squire JM, Liu J. Direct visualization of myosin-binding protein C bridging myosin and actin filaments in intact muscle. Proceedings Of The National Academy Of Sciences Of The United States Of America 2011, 108: 11423-11428. PMID: 21705660, PMCID: PMC3136262, DOI: 10.1073/pnas.1103216108.Peer-Reviewed Original Research
2010
Electron Tomography of Cryofixed, Isometrically Contracting Insect Flight Muscle Reveals Novel Actin-Myosin Interactions
Wu S, Liu J, Reedy MC, Tregear RT, Winkler H, Franzini-Armstrong C, Sasaki H, Lucaveche C, Goldman YE, Reedy MK, Taylor KA. Electron Tomography of Cryofixed, Isometrically Contracting Insect Flight Muscle Reveals Novel Actin-Myosin Interactions. PLOS ONE 2010, 5: e12643. PMID: 20844746, PMCID: PMC2936580, DOI: 10.1371/journal.pone.0012643.Peer-Reviewed Original Research
2009
Methods for identifying and averaging variable molecular conformations in tomograms of actively contracting insect flight muscle
Wu S, Liu J, Reedy MC, Winkler H, Reedy MK, Taylor KA. Methods for identifying and averaging variable molecular conformations in tomograms of actively contracting insect flight muscle. Journal Of Structural Biology 2009, 168: 485-502. PMID: 19698791, PMCID: PMC2805068, DOI: 10.1016/j.jsb.2009.08.007.Peer-Reviewed Original Research
2008
The 3D Structure of Villin as an Unusual F-Actin Crosslinker
Hampton CM, Liu J, Taylor DW, DeRosier DJ, Taylor KA. The 3D Structure of Villin as an Unusual F-Actin Crosslinker. Structure 2008, 16: 1882-1891. PMID: 19081064, PMCID: PMC2782859, DOI: 10.1016/j.str.2008.09.015.Peer-Reviewed Original ResearchMeSH KeywordsActin CytoskeletonActinsAmino Acid SequenceAnimalsBinding SitesChickensCross-Linking ReagentsImage Processing, Computer-AssistedImaging, Three-DimensionalIntestinesMicrofilament ProteinsModels, MolecularMolecular Sequence DataMolecular WeightMuscle, SkeletalProtein BindingProtein Structure, SecondaryProtein Structure, TertiaryRabbitsSequence Homology, Amino AcidTomographic subvolume alignment and subvolume classification applied to myosin V and SIV envelope spikes
Winkler H, Zhu P, Liu J, Ye F, Roux KH, Taylor KA. Tomographic subvolume alignment and subvolume classification applied to myosin V and SIV envelope spikes. Journal Of Structural Biology 2008, 165: 64-77. PMID: 19032983, PMCID: PMC2656979, DOI: 10.1016/j.jsb.2008.10.004.Peer-Reviewed Original ResearchThe role of filament-packing dynamics in powering amoeboid cell motility
Miao L, Vanderlinde O, Liu J, Grant RP, Wouterse A, Shimabukuro K, Philipse A, Stewart M, Roberts TM. The role of filament-packing dynamics in powering amoeboid cell motility. Proceedings Of The National Academy Of Sciences Of The United States Of America 2008, 105: 5390-5395. PMID: 18385381, PMCID: PMC2291107, DOI: 10.1073/pnas.0708416105.Peer-Reviewed Original Research
2006
Electron Tomography of Swollen Rigor Fibers of Insect Flight Muscle Reveals a Short and Variably Angled S2 Domain
Liu J, Wu S, Reedy MC, Winkler H, Lucaveche C, Cheng Y, Reedy MK, Taylor KA. Electron Tomography of Swollen Rigor Fibers of Insect Flight Muscle Reveals a Short and Variably Angled S2 Domain. Journal Of Molecular Biology 2006, 362: 844-860. PMID: 16949613, DOI: 10.1016/j.jmb.2006.07.084.Peer-Reviewed Original Research
2004
A 3-D Reconstruction of Smooth Muscle α-Actinin by CryoEm Reveals Two Different Conformations at the Actin-binding Region
Liu J, Taylor DW, Taylor KA. A 3-D Reconstruction of Smooth Muscle α-Actinin by CryoEm Reveals Two Different Conformations at the Actin-binding Region. Journal Of Molecular Biology 2004, 338: 115-125. PMID: 15050827, DOI: 10.1016/j.jmb.2004.02.034.Peer-Reviewed Original Research