2020
Albumin fusion with granulocyte-macrophage colony-stimulating factor acts as an immunotherapy against chronic tuberculosis
Chuang YM, He L, Pinn ML, Tsai YC, Cheng MA, Farmer E, Karakousis PC, Hung CF. Albumin fusion with granulocyte-macrophage colony-stimulating factor acts as an immunotherapy against chronic tuberculosis. Cellular & Molecular Immunology 2020, 18: 2393-2401. PMID: 32382128, PMCID: PMC8484439, DOI: 10.1038/s41423-020-0439-2.Peer-Reviewed Original ResearchConceptsTuberculosis infectionChronic tuberculosis infectionPotent immune responsesGM-CSFLymph nodesDendritic cellsImmune responseChronic Mycobacterium tuberculosis infectionHigher IL-1β levelsAlbumin fusionLung bacillary burdenTB treatment regimensDendritic cell populationsDrug-resistant TBIL-1β levelsGM-CSF administrationMycobacterium tuberculosis infectionNaive T cellsIL-1β releaseBacillary burdenChronic tuberculosisNovel immunotherapiesTreatment regimensVaccination platformSubcutaneous administration
2016
Stringent Response Factors PPX1 and PPK2 Play an Important Role in Mycobacterium tuberculosis Metabolism, Biofilm Formation, and Sensitivity to Isoniazid In Vivo
Chuang YM, Dutta NK, Hung CF, Wu TC, Rubin H, Karakousis PC. Stringent Response Factors PPX1 and PPK2 Play an Important Role in Mycobacterium tuberculosis Metabolism, Biofilm Formation, and Sensitivity to Isoniazid In Vivo. Antimicrobial Agents And Chemotherapy 2016, 60: 6460-6470. PMID: 27527086, PMCID: PMC5075050, DOI: 10.1128/aac.01139-16.Peer-Reviewed Original ResearchMeSH KeywordsAcid Anhydride HydrolasesAnimalsAntitubercular AgentsBiofilmsCitric Acid CycleClofazimineDisease Models, AnimalDrug Resistance, BacterialGene ExpressionGlycerophosphatesIsoenzymesIsoniazidMeropenemMiceMycobacterium tuberculosisNaphthoquinonesPhosphotransferases (Phosphate Group Acceptor)PolyphosphatesThienamycinsTuberculosis VaccinesTuberculosis, Multidrug-ResistantVaccines, DNAXyloseConceptsM. tuberculosisVaccine-induced immunityGlobal health threatActivity of isoniazidMedical nonadherenceChronic tuberculosisProlonged therapyDNA vaccineAntibiotic treatmentMycobacterium tuberculosis metabolismReal-time PCRMurine modelM. tuberculosis metabolismAntibiotic toleranceLow intracellular levelsProtective activityAntibiotic sensitivityDrug resistanceTuberculosisLiquid chromatography-tandem mass spectrometryMycobacterium tuberculosisHealth threatKey regulatory moleculesChromatography-tandem mass spectrometryEnhanced susceptibility