Endosome-ER Contacts Control Actin Nucleation and Retromer Function through VAP-Dependent Regulation of PI4P
Dong R, Saheki Y, Swarup S, Lucast L, Harper JW, De Camilli P. Endosome-ER Contacts Control Actin Nucleation and Retromer Function through VAP-Dependent Regulation of PI4P. Cell 2016, 166: 408-423. PMID: 27419871, PMCID: PMC4963242, DOI: 10.1016/j.cell.2016.06.037.Peer-Reviewed Original ResearchMeSH KeywordsActinsEndoplasmic ReticulumEndosomesGene Knockout TechniquesHeLa CellsHumansMicrofilament ProteinsPhosphatidylinositol PhosphatesReceptors, SteroidTranscription Activator-Like Effector NucleasesVesicular Transport ProteinsControl of plasma membrane lipid homeostasis by the extended synaptotagmins
Saheki Y, Bian X, Schauder CM, Sawaki Y, Surma MA, Klose C, Pincet F, Reinisch KM, De Camilli P. Control of plasma membrane lipid homeostasis by the extended synaptotagmins. Nature Cell Biology 2016, 18: 504-515. PMID: 27065097, PMCID: PMC4848133, DOI: 10.1038/ncb3339.Peer-Reviewed Original ResearchMeSH KeywordsBiological TransportCalciumCarrier ProteinsCell MembraneCRISPR-Cas SystemsCytosolDiglyceridesEndoplasmic ReticulumEnzyme ActivationGene Knockout TechniquesHeLa CellsHomeostasisHumansMembrane LipidsSynaptotagminsTranscription Activator-Like Effector NucleasesType C PhospholipasesConceptsSMP domainE-Syt1ER-PM tethersMembrane lipid homeostasisPlasma membrane lipidsEndoplasmic reticulum proteinAccumulation of diacylglycerolE-SytsExtended synaptotagminsMolecular basisMajor glycerolipidsReticulum proteinsMetabolic recyclingMembrane lipidsLipid homeostasisPLC activationSynaptotagminSustained accumulationHomeostatic responseDiacylglycerolGlycerolipidsMetabolic changesGenomeCa2Accumulation