1996
Alzheimer Amyloid Protein Precursor Is Localized in Nerve Terminal Preparations to Rab5-containing Vesicular Organelles Distinct from Those Implicated in the Synaptic Vesicle Pathway*
Ikin A, Annaert W, Takei K, De Camilli P, Jahn R, Greengard P, Buxbaum J. Alzheimer Amyloid Protein Precursor Is Localized in Nerve Terminal Preparations to Rab5-containing Vesicular Organelles Distinct from Those Implicated in the Synaptic Vesicle Pathway*. Journal Of Biological Chemistry 1996, 271: 31783-31786. PMID: 8943215, DOI: 10.1074/jbc.271.50.31783.Peer-Reviewed Original ResearchConceptsAmyloid protein precursorSmall synaptic vesiclesSynaptic vesiclesVesicular organellesProtein precursorSynaptic vesicle recycling pathwayNerve terminal preparationsSynaptic vesicle pathwayVesicle recycling pathwayAlzheimer amyloid protein precursorRecycling pathwayVesicle pathwayOrganellesVesiclesImmunoisolatesPathwayHomogeneous populationRab5Nerve terminalsHigh levelsDistinctPrecursorsSubstantial numberBilamellar vesicles
1989
Synaptic vesicles immunoisolated from rat cerebral cortex contain high levels of glutamate
Burger P, Mehl E, Cameron P, Maycox P, Baumert M, Lottspeich F, De Camilli P, Jahn R. Synaptic vesicles immunoisolated from rat cerebral cortex contain high levels of glutamate. Neuron 1989, 3: 715-720. PMID: 2577130, DOI: 10.1016/0896-6273(89)90240-7.Peer-Reviewed Original ResearchConceptsSynaptic vesiclesRat cerebral cortexRat brain synaptic vesiclesProton electrochemical gradientBrain synaptic vesiclesCerebral cortexCytosolic poolMajor excitatory neurotransmitterVesicle membraneElectrochemical gradientAmino acidsVesiclesVesicle hypothesisMammalian CNSExcitatory neurotransmitterGlutamate lossEnrichment of glutamateN-ethylmaleimideStarting fractionGlutamate contentGlutamateHigh levelsL-glutamateCortexExchange experiments