2021
Stepwise membrane binding of extended synaptotagmins revealed by optical tweezers
Ge J, Bian X, Ma L, Cai Y, Li Y, Yang J, Karatekin E, De Camilli P, Zhang Y. Stepwise membrane binding of extended synaptotagmins revealed by optical tweezers. Nature Chemical Biology 2021, 18: 313-320. PMID: 34916620, PMCID: PMC8891060, DOI: 10.1038/s41589-021-00914-3.Peer-Reviewed Original Research
2016
Control of plasma membrane lipid homeostasis by the extended synaptotagmins
Saheki Y, Bian X, Schauder CM, Sawaki Y, Surma MA, Klose C, Pincet F, Reinisch KM, De Camilli P. Control of plasma membrane lipid homeostasis by the extended synaptotagmins. Nature Cell Biology 2016, 18: 504-515. PMID: 27065097, PMCID: PMC4848133, DOI: 10.1038/ncb3339.Peer-Reviewed Original ResearchConceptsSMP domainE-Syt1ER-PM tethersMembrane lipid homeostasisPlasma membrane lipidsEndoplasmic reticulum proteinAccumulation of diacylglycerolE-SytsExtended synaptotagminsMolecular basisMajor glycerolipidsReticulum proteinsMetabolic recyclingMembrane lipidsLipid homeostasisPLC activationSynaptotagminSustained accumulationHomeostatic responseDiacylglycerolGlycerolipidsMetabolic changesGenomeCa2AccumulationExtended‐Synaptotagmins as Lipid Transporters at ER‐PM Contact Sites
Reinisch K, Schauder C, Wu X, Saheki Y, Narayanaswamy P, Torta F, Wenk M, De Camilli P. Extended‐Synaptotagmins as Lipid Transporters at ER‐PM Contact Sites. The FASEB Journal 2016, 30 DOI: 10.1096/fasebj.30.1_supplement.115.2.Peer-Reviewed Original ResearchMembrane contact sitesSMP domainContact sitesExtended synaptotagminsEndoplasmic reticulumPlasma membraneER-PM contact sitesLipid transportLipid transferLipid-binding modulesER-PM tethersΒ-barrel structureExchange of lipidsERMES complexE-SytsProtein modulesProtein domainsVesicular transportC2 domainÅ resolutionLipid transportersHydrophobic residuesUehara Memorial FoundationSynaptotagmin 2Direct role
2015
Phosphoinositide Signaling in the Control of Membrane Dynamics and Interactions
De Camilli P. Phosphoinositide Signaling in the Control of Membrane Dynamics and Interactions. The FASEB Journal 2015, 29 DOI: 10.1096/fasebj.29.1_supplement.90.1.Peer-Reviewed Original ResearchPlasma membraneEndoplasmic reticulumIntrinsic membrane proteinsKey regulatory roleIntracellular second messengerMembrane identityE-SytsExtended synaptotagminsDifferent phosphoinositidesEndocytic membranesPutative functionsMembrane proteinsCell physiologyMembrane dynamicsCytosolic proteinsSecond messengerRegulatory rolePhosphatidyl inositolPhosphoinositideCell membraneInositol groupProteinMembranePhosphorylated metabolitesCa 2