2000
Hepatocyte Growth Factor Overexpression in the Islet of Transgenic Mice Increases Beta Cell Proliferation, Enhances Islet Mass, and Induces Mild Hypoglycemia*
Garcia-Ocaña A, Takane K, Syed M, Philbrick W, Vasavada R, Stewart A. Hepatocyte Growth Factor Overexpression in the Islet of Transgenic Mice Increases Beta Cell Proliferation, Enhances Islet Mass, and Induces Mild Hypoglycemia*. Journal Of Biological Chemistry 2000, 275: 1226-1232. PMID: 10625667, DOI: 10.1074/jbc.275.2.1226.Peer-Reviewed Original ResearchConceptsHepatocyte growth factorRat insulin II promoterBeta-cell proliferationDiabetogenic effectMild hypoglycemiaIslet massInsulin contentOverexpression of HGFIslet numberInsulin productionBeta-cell replication rateRole of HGFHepatocyte growth factor overexpressionProliferation of isletsBeta-cell massCell proliferationGrowth factor overexpressionMesenchyme-derived cellsPancreatic areaCell replication ratesFasting statesNormal littermatesTransgenic miceHGF mRNAIslet cells
1999
Regulation of parathyroid hormone-related protein gene expression in murine keratinocytes by E1A isoforms: a role for basal promoter and Ets-1 site
Foley J, Wysolmerski J, Missero C, King C, Philbrick W. Regulation of parathyroid hormone-related protein gene expression in murine keratinocytes by E1A isoforms: a role for basal promoter and Ets-1 site. Molecular And Cellular Endocrinology 1999, 156: 13-23. PMID: 10612419, DOI: 10.1016/s0303-7207(99)00151-3.Peer-Reviewed Original ResearchMeSH KeywordsAdenovirus E1A ProteinsAnimalsBase SequenceBinding SitesCell Line, TransformedEnhancer Elements, GeneticGene Expression RegulationGenes, rasGenes, ReporterKeratinocytesLuciferasesMiceParathyroid HormoneParathyroid Hormone-Related ProteinPromoter Regions, GeneticProtein IsoformsProteinsProto-Oncogene Protein c-ets-1Proto-Oncogene ProteinsProto-Oncogene Proteins c-etsRNA, MessengerTranscription FactorsTranscription, GeneticConceptsGene expressionBasal promoterReporter constructsEts-1 siteMinimal promoter regionProtein gene expressionTransient transfection assaysReporter gene activityRepressor domainE1A oncoproteinGene activityRas transformationE1A isoformsMurine keratinocyte lineSite upstreamReporter geneTransfection assaysPromoter regionParathyroid hormone-related protein gene expression
1997
Bcl-2 Lies Downstream of Parathyroid Hormone–related Peptide in a Signaling Pathway That Regulates Chondrocyte Maturation during Skeletal Development
Amling M, Neff L, Tanaka S, Inoue D, Kuida K, Weir E, Philbrick W, Broadus A, Baron R. Bcl-2 Lies Downstream of Parathyroid Hormone–related Peptide in a Signaling Pathway That Regulates Chondrocyte Maturation during Skeletal Development. Journal Of Cell Biology 1997, 136: 205-213. PMID: 9008714, PMCID: PMC2132464, DOI: 10.1083/jcb.136.1.205.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsApoptosisBcl-2-Associated X ProteinBone DevelopmentCartilageCells, CulturedCollagenGene ExpressionGrowth PlateHumansHypertrophyMiceMice, KnockoutMice, TransgenicOrgan SpecificityParathyroid Hormone-Related ProteinPromoter Regions, GeneticProteinsProto-Oncogene ProteinsProto-Oncogene Proteins c-bcl-2Recombinant Fusion ProteinsSignal TransductionConceptsSkeletal developmentChondrocyte maturationBcl-2Cell deathCollagen II promoterApoptotic cell deathMutant PTH/PTHrP receptorsDevelopmental programMolecular mechanismsSignaling pathwaysCell typesGrowth plate chondrocytesTargeted disruptionGenesMetaphyseal chondrodysplasiaMaturationPTHrP geneTransgenic micePathwayPTH/PTHrP receptorAccelerated maturationSkeletal dysplasiaHormone-related peptideMajor roleChondrocytes
1996
Targeted overexpression of parathyroid hormone-related peptide in chondrocytes causes chondrodysplasia and delayed endochondral bone formation.
Weir E, Philbrick W, Amling M, Neff L, Baron R, Broadus A. Targeted overexpression of parathyroid hormone-related peptide in chondrocytes causes chondrodysplasia and delayed endochondral bone formation. Proceedings Of The National Academy Of Sciences Of The United States Of America 1996, 93: 10240-10245. PMID: 8816783, PMCID: PMC38368, DOI: 10.1073/pnas.93.19.10240.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBone DevelopmentDwarfismGene Expression Regulation, DevelopmentalGrowth PlateHumansMiceMice, Inbred C57BLMice, Inbred StrainsMice, TransgenicOsteochondrodysplasiasParathyroid HormoneParathyroid Hormone-Related ProteinPolymerase Chain ReactionProcollagenPromoter Regions, GeneticProtein BiosynthesisProteinsRegulatory Sequences, Nucleic AcidTranscription, GeneticConceptsPTH/PTHrP receptorHormone-related peptideParathyroid hormonePTHrP receptorParathyroid hormone-related peptideChondrocyte differentiationOverexpression of PTHrPType II collagen promoterEndochondral ossificationShort-limbed dwarfismParaneoplastic hypercalcemiaPTHrP functionsBone collar formationMalignant tumorsDevelopmental regulatory moleculeEndochondral bone formationTransgenic micePTHrPCommon receptorEndochondral bone developmentTargeted overexpressionFetal tissuesPTHrP geneFollicle formationNormal bone