2006
Neuroprotection by PlGF gene-modified human mesenchymal stem cells after cerebral ischaemia
Liu H, Honmou O, Harada K, Nakamura K, Houkin K, Hamada H, Kocsis J. Neuroprotection by PlGF gene-modified human mesenchymal stem cells after cerebral ischaemia. Brain 2006, 129: 2734-2745. PMID: 16901914, PMCID: PMC2605397, DOI: 10.1093/brain/awl207.Peer-Reviewed Original ResearchMeSH KeywordsAdenoviridaeAnimalsApoptosisBrain IschemiaGenetic VectorsHumansImage Processing, Computer-AssistedInfarction, Middle Cerebral ArteryMagnetic Resonance ImagingMaleMesenchymal Stem Cell TransplantationMesenchymal Stem CellsModels, AnimalNeovascularization, PathologicNeuropsychological TestsPlacenta Growth FactorPregnancy ProteinsRatsRats, Sprague-DawleyTransduction, GeneticConceptsMiddle cerebral artery occlusionCerebral ischaemiaMesenchymal stem cellsIntravenous deliveryPermanent middle cerebral artery occlusionHuman MSCsControl sham groupLimb placement testRats 3 hCerebral artery occlusionTreadmill stress testPlacental growth factorIntraluminal vascular occlusionEnzyme-linked immunosorbentNon-neural tissuesHuman mesenchymal stem cellsStem cellsAdult bone marrowArtery occlusionInfarcted hemisphereSham groupFunctional outcomeVascular occlusionFunctional deficitsInfarction size
2005
I.v. infusion of brain-derived neurotrophic factor gene-modified human mesenchymal stem cells protects against injury in a cerebral ischemia model in adult rat
Nomura T, Honmou O, Harada K, Houkin K, Hamada H, Kocsis J. I.v. infusion of brain-derived neurotrophic factor gene-modified human mesenchymal stem cells protects against injury in a cerebral ischemia model in adult rat. Neuroscience 2005, 136: 161-169. PMID: 16229956, PMCID: PMC2605391, DOI: 10.1016/j.neuroscience.2005.06.062.Peer-Reviewed Original ResearchConceptsBrain-derived neurotrophic factorMesenchymal stem cell groupBrain-derived neurotrophic factor contributesMiddle cerebral artery occlusionStem cell groupCerebral artery occlusionCerebral ischemia modelMesenchymal stem cellsNeurotrophic factorArtery occlusionCell groupsIschemia modelPermanent middle cerebral artery occlusionRat cerebral ischemia modelHuman mesenchymal stem cellsBrain-derived neurotrophic factor (BDNF) geneStem cellsControl sham groupTreadmill stress testRats 6 hFactor contributesNeurotrophic factor geneIntraluminal vascular occlusionAdult bone marrowCerebral ischemiaIntravenous infusion of immortalized human mesenchymal stem cells protects against injury in a cerebral ischemia model in adult rat
Honma T, Honmou O, Iihoshi S, Harada K, Houkin K, Hamada H, Kocsis J. Intravenous infusion of immortalized human mesenchymal stem cells protects against injury in a cerebral ischemia model in adult rat. Experimental Neurology 2005, 199: 56-66. PMID: 15967439, PMCID: PMC2605388, DOI: 10.1016/j.expneurol.2005.05.004.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigens, CDBehavior, AnimalBrain IschemiaCell CountCells, CulturedDisease Models, AnimalExercise TestGreen Fluorescent ProteinsHumansImmunohistochemistryInfusions, IntravenousMagnetic Resonance ImagingMagnetic Resonance SpectroscopyMaleMaze LearningMesenchymal Stem Cell TransplantationMesenchymal Stem CellsPhosphopyruvate HydrataseRatsRats, Sprague-DawleyTime FactorsConceptsMiddle cerebral artery occlusionIntravenous infusionFunctional outcomeLesion sizeTransient middle cerebral artery occlusionMesenchymal stem cellsCerebral artery occlusionCerebral infarction volumeCerebral ischemia modelSpinal cord injurySubsequent histological examinationPotential therapeutic benefitRats 12 hMorris water mazeBone marrow cellsHuman mesenchymal stem cellsStem cellsHTERT-MSCsArtery occlusionInfarction volumeCerebral ischemiaCord injuryFunctional improvementLesion volumeIschemia model
2001
Transplantation of Clonal Neural Precursor Cells Derived from Adult Human Brain Establishes Functional Peripheral Myelin in the Rat Spinal Cord
Akiyama Y, Honmou O, Kato T, Uede T, Hashi K, Kocsis J. Transplantation of Clonal Neural Precursor Cells Derived from Adult Human Brain Establishes Functional Peripheral Myelin in the Rat Spinal Cord. Experimental Neurology 2001, 167: 27-39. PMID: 11161590, DOI: 10.1006/exnr.2000.7539.Peer-Reviewed Original ResearchMeSH KeywordsAdultAnimalsBrainBrain Tissue TransplantationCell DifferentiationCells, CulturedCerebral VentriclesClone CellsDemyelinating DiseasesFemaleGenes, ReporterHumansIntermediate Filament ProteinsMaleMiddle AgedMyelin SheathNerve Tissue ProteinsNestinNeural ConductionNeuronsRadiation Injuries, ExperimentalRatsRats, WistarSpinal CordStem Cell TransplantationStem CellsTransplantation, HeterologousConceptsAdult human brainRat spinal cordNestin-positive cellsNeural precursor cellsSpinal cordAdult rat spinal cordNormal conduction velocityHuman brainMitogen withdrawalSpinal cord resultsPrecursor cellsGlia-like cellsSchwann cell myelinationNeural progenitor cellsFunctional remyelinationP0 immunoreactivityRemyelinated axonsExtensive remyelinationCord resultsAnterior hornLateral ventricleSubventricular zoneMajority of cellsMyelin patternSchwann cells
1998
Mechanisms of enhancement of neurite regeneration in vitro following a conditioning sciatic nerve lesion
Lankford K, Waxman S, Kocsis J. Mechanisms of enhancement of neurite regeneration in vitro following a conditioning sciatic nerve lesion. The Journal Of Comparative Neurology 1998, 391: 11-29. PMID: 9527536, PMCID: PMC2605358, DOI: 10.1002/(sici)1096-9861(19980202)391:1<11::aid-cne2>3.0.co;2-u.Peer-Reviewed Original ResearchConceptsDorsal root gangliaConditioning lesionNerve injuryNerve regenerationAffected dorsal root ganglionControl dorsal root gangliaDenervated peripheral nervePrior nerve injurySciatic nerve lesionCultured DRG neuronsSciatic nerve transectionPeripheral target tissuesPeripheral nerve stumpRapid nerve regenerationAbility of neuronsSecond axotomyNerve lesionsDRG neuronsNerve transectionNerve stumpRoot gangliaControl neuronsPeripheral nervesNerve tractsAdult rats
1996
The α3 Isoform Protein of the Na+,K+-ATPase Is Associated With the Sites of Cardiac and Neuromuscular Impulse Transmission
Zahler R, Sun W, Ardito T, Zhang Z, Kocsis J, Kashgarian M. The α3 Isoform Protein of the Na+,K+-ATPase Is Associated With the Sites of Cardiac and Neuromuscular Impulse Transmission. Circulation Research 1996, 78: 870-879. PMID: 8620608, DOI: 10.1161/01.res.78.5.870.Peer-Reviewed Original ResearchConceptsNeuromuscular junctionConduction systemImpulse transmissionIsoform-specific antibodiesSkeletal muscle expressesSkeletal musclePresynaptic motor terminalsCardiac conduction systemHeart conduction systemAlpha subunitAxonal processesRat heartCardiac myocytesIsoform mRNAsMuscleClassic morphologyJunctional complexesImmunoreactive proteinPump isoformsAntibodiesIsoform proteinsHeartPump proteinImmunohistochemistryIsoforms
1992
Conduction properties of spinal cord axons in the myelin-deficient rat mutant
Utzschneider D, Black J, Kocsis J. Conduction properties of spinal cord axons in the myelin-deficient rat mutant. Neuroscience 1992, 49: 221-228. PMID: 1407548, DOI: 10.1016/0306-4522(92)90090-o.Peer-Reviewed Original ResearchConceptsDorsal column axonsMyelin-deficient rat mutantsSpinal cordFrequency-dependent conduction blockNormal age-matched ratsBrain slice chamberMyelin-deficient rat spinal cordAction potential dischargeRat mutantAge-matched ratsRat spinal cordSpinal cord axonsAction potential conductionControl ratsDemyelinated axonsConduction blockConduction velocityRefractory periodPotential dischargeControl axonsSlice chamberPharmacological resultsPotential conductionAxonsCompensatory mechanisms
1988
Buspirone, 8-OH-DPAT and ipsapirone: effects on hippocampal cerebellar and sciatic fiber excitability
Hiner B, Mauk M, Peroutka S, Kocsis J. Buspirone, 8-OH-DPAT and ipsapirone: effects on hippocampal cerebellar and sciatic fiber excitability. Brain Research 1988, 461: 1-9. PMID: 2906267, DOI: 10.1016/0006-8993(88)90719-6.Peer-Reviewed Original ResearchConceptsCerebellar parallel fibersSerotonergic mechanismsNerve excitabilityFiber excitabilityAction potentialsSucrose gap recordingsEffects of serotoninNovel anxiolytic buspironeCompound action potentialDose-dependent reductionSchaffer collateral fibersParallel fibersAfferent fibersSciatic nerveReversible decrementsCollateral fibersAnxiolytic buspironeConduction velocityDPATIpsapironeGap recordingsBuspironeCerebellar neuronsAxonal responseExcitability
1987
Nodal spacing along regenerated axons following a crush lesion of the developing rat sciatic nerve.
Hildebrand C, Mustafa G, Bowe C, Kocsis J. Nodal spacing along regenerated axons following a crush lesion of the developing rat sciatic nerve. Brain Research 1987, 429: 147-54. PMID: 3567658, DOI: 10.1016/0165-3806(87)90148-9.Peer-Reviewed Original ResearchConceptsRat sciatic nerveSciatic nerveRegenerated nervesCrush lesionRegenerated rat sciatic nerveNewborn rat pupsSciatic nerve axonsPostnatal ageRegenerated axonsPostnatal eventsRat pupsNerveNerve axonsMyelin sheathL increaseAxonsWeeksBirthAgeLesionsMyelinationSignsRemodellingSuch signsLength growth
1985
Myelin sheath remodelling in regenerated rat sciatic nerve
Hildebrand C, Kocsis J, Berglund S, Waxman S. Myelin sheath remodelling in regenerated rat sciatic nerve. Brain Research 1985, 358: 163-170. PMID: 2416385, DOI: 10.1016/0006-8993(85)90960-6.Peer-Reviewed Original ResearchConceptsRat sciatic nerveSciatic nerveRegenerated nervesAdult rat sciatic nerveRegenerated rat sciatic nerveNormal control nervesLight microscopic examinationAction potential waveformCrush lesionMonths survivalNerve segmentsControl nervesSame nerveIndividual nervesNerve fibersNerveShort sheathMyelin layersMyelin sheathPotassium channelsMicroscopic examination
1983
Myelin protein metabolism in demyelination and remyelination in the sciatic nerve
Smith M, Kocsis J, Waxman S. Myelin protein metabolism in demyelination and remyelination in the sciatic nerve. Brain Research 1983, 270: 37-44. PMID: 6871715, DOI: 10.1016/0006-8993(83)90789-8.Peer-Reviewed Original ResearchConceptsMyelin proteinsControl nervesLPC injectionSciatic nerveRight sciatic nerveSeries of ratsLeft nerveSchwann cellsNerveStructural myelin proteinsLPC treatmentFirst weekTime pointsAmino acid incorporationProtein metabolismLabeled amino acidsAcid incorporationMyelinDaysInjectionLysophosphatidylcholineDemyelinationRemyelinationProteinRatsThe supernormal period of the cerebellar parallel fibers effects of [Ca2+]o and [K+]o
Malenka R, Kocsis J, Waxman S. The supernormal period of the cerebellar parallel fibers effects of [Ca2+]o and [K+]o. Pflügers Archiv - European Journal Of Physiology 1983, 397: 176-183. PMID: 6878005, DOI: 10.1007/bf00584354.Peer-Reviewed Original ResearchConceptsSupernormal periodConditioning stimulationActivity-dependent changesParallel fibersCerebellar parallel fibersConditioning volleyIon-sensitive microelectrodesConditioning stimulusExtracellular calciumLatency changesLatency shiftCortex exhibitExtracellular ionic concentrationsTest response latenciesTest latencyStimulationResponse latencyRelative increaseSmall increaseLatencyPeriodSuperfusateExcitabilityEffects of extracellular potassium concentration on the excitability of the parallel fibres of the rat cerebellum.
Kocsis J, Malenka R, Waxman S. Effects of extracellular potassium concentration on the excitability of the parallel fibres of the rat cerebellum. The Journal Of Physiology 1983, 334: 225-244. PMID: 6864558, PMCID: PMC1197311, DOI: 10.1113/jphysiol.1983.sp014491.Peer-Reviewed Original Research
1982
Regenerating mammalian nerve fibres: changes in action potential waveform and firing characteristics following blockage of potassium conductance
Kocsis J, Waxman S, Hildebrand C, Ruiz J. Regenerating mammalian nerve fibres: changes in action potential waveform and firing characteristics following blockage of potassium conductance. Proceedings Of The Royal Society B 1982, 217: 77-87. PMID: 6131423, DOI: 10.1098/rspb.1982.0095.Peer-Reviewed Original ResearchConceptsRegenerating axonsNerve fibersFiring propertiesAction potentialsPotassium conductancePotassium channelsCompound action potentialSciatic nerve fibersEarly regenerating axonsAction potential waveformRat nerve fibresMammalian nerve fibresDemyelinated axonsMyelinated fibersExtracellular applicationAxonsRecording techniquesSingle stimulusFiring characteristicsPotential waveformPresent studyEffects of GABA on stimulus-evoked changes in [K+]o and parallel fiber excitability.
Malenka R, Kocsis J. Effects of GABA on stimulus-evoked changes in [K+]o and parallel fiber excitability. Journal Of Neurophysiology 1982, 48: 608-621. PMID: 6290614, DOI: 10.1152/jn.1982.48.3.608.Peer-Reviewed Original ResearchIntra-axonal recordings in rat dorsal column axons: membrane hyperpolarization and decreased excitability precede the primary afferent depolarization
Kocsis J, Waxman S. Intra-axonal recordings in rat dorsal column axons: membrane hyperpolarization and decreased excitability precede the primary afferent depolarization. Brain Research 1982, 238: 222-227. PMID: 6282392, DOI: 10.1016/0006-8993(82)90787-9.Peer-Reviewed Original ResearchConceptsPrimary afferent depolarizationIntra-axonal recordingsRat lumbosacral spinal cordDorsal column stimulationDorsal root potentialsLumbosacral spinal cordDorsal column axonsDorsal columnsDorsal rootsAxonal depolarizationRoot potentialsSpinal cordGABA responsesNeuronal elementsIntracellular correlatesProlonged depolarizationMembrane hyperpolarizationHyperpolarizationDepolarizationExcitability
1981
Modulation of Parallel Fiber Excitability by Postsynaptically Mediated Changes in Extracellular Potassium
Malenka R, Kocsis J, Ransom B, Waxman S. Modulation of Parallel Fiber Excitability by Postsynaptically Mediated Changes in Extracellular Potassium. Science 1981, 214: 339-341. PMID: 7280695, DOI: 10.1126/science.7280695.Peer-Reviewed Original ResearchConceptsSynaptic field potentialsField potentialsParallel fiber stimulationExtracellular potassium concentrationRat cerebellar cortexParallel fibersAfferent fibersPostsynaptic elementsFiber excitabilityCerebellar cortexExtracellular potassiumFiber stimulationExcitabilityMarked increasePotassium concentrationStimulationMolecular layer
1980
Effects of 4-aminopyridine on the frequency following properties of the parallel fibers of the cerebellar cortex
Kocsis J, Malenka R, Waxman S. Effects of 4-aminopyridine on the frequency following properties of the parallel fibers of the cerebellar cortex. Brain Research 1980, 195: 511-516. PMID: 6249447, DOI: 10.1016/0006-8993(80)90090-6.Peer-Reviewed Original Research
1979
A supernormal period in central axons following single cell stimulation
Kocsis J, VanderMaelen C. A supernormal period in central axons following single cell stimulation. Experimental Brain Research 1979, 36: 381-386. PMID: 488207, DOI: 10.1007/bf00238919.Peer-Reviewed Original Research