2023
Repeated intravenous infusion of mesenchymal stem cells enhances recovery of motor function in a rat model with chronic spinal cord injury
Kurihara K, Sasaki M, Nagahama H, Obara H, Fukushi R, Hirota R, Yoshimoto M, Teramoto A, Kocsis J, Yamashita T, Honmou O. Repeated intravenous infusion of mesenchymal stem cells enhances recovery of motor function in a rat model with chronic spinal cord injury. Brain Research 2023, 1817: 148484. PMID: 37442249, DOI: 10.1016/j.brainres.2023.148484.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsDiffusion Tensor ImagingInfusions, IntravenousMesenchymal Stem Cell TransplantationMesenchymal Stem CellsPyramidal TractsRatsRecovery of FunctionSpinal CordSpinal Cord InjuriesConceptsSpinal cord injuryInfusion of MSCsChronic spinal cord injuryMesenchymal stem cellsAxonal sproutingCord injuryHD-MSCsIntravenous infusionMotor functionVehicle groupWeek 6Single high-dose infusionInjection of MSCsChronic SCI ratsSingle MSC injectionGreater functional recoveryRat SCI modelSingle intravenous infusionHigh-dose infusionLimited treatment optionsGreater functional improvementSingle high doseHigh disease burdenVivo diffusion tensor imagingEx vivo diffusion tensor imagingTherapeutic efficacy of intravenous infusion of mesenchymal stem cells in rat perinatal brain injury
Terada K, Sasaki M, Nagahama H, Kataoka-Sasaki Y, Oka S, Ukai R, Yokoyama T, Iizuka Y, Sakai T, Fukumura S, Tsugawa T, Kocsis J, Honmou O. Therapeutic efficacy of intravenous infusion of mesenchymal stem cells in rat perinatal brain injury. Pediatric Research 2023, 94: 1921-1928. PMID: 37422495, DOI: 10.1038/s41390-023-02717-9.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBrain InjuriesDisease Models, AnimalHumansInfant, NewbornInfant, PrematureInfusions, IntravenousMesenchymal Stem Cell TransplantationMesenchymal Stem CellsRatsRats, Sprague-DawleyConceptsPerinatal brain injuryBrain injuryMesenchymal stem cellsIntravenous infusionVehicle groupBrain volumeTherapeutic efficacyInfused mesenchymal stem cellsLeft common carotid arteryHistological analysisNon-ischemic hemispherePostnatal day 7Common carotid arteryEmbryonic day 18Stem cellsHypoxia-ischemiaMSC infusionPreterm infantsGABAergic cellsNeurological functionSignificant complicationsCortical synapsesFunctional improvementCarotid arteryIntravenous administration
2006
Neuroprotection by PlGF gene-modified human mesenchymal stem cells after cerebral ischaemia
Liu H, Honmou O, Harada K, Nakamura K, Houkin K, Hamada H, Kocsis J. Neuroprotection by PlGF gene-modified human mesenchymal stem cells after cerebral ischaemia. Brain 2006, 129: 2734-2745. PMID: 16901914, PMCID: PMC2605397, DOI: 10.1093/brain/awl207.Peer-Reviewed Original ResearchMeSH KeywordsAdenoviridaeAnimalsApoptosisBrain IschemiaGenetic VectorsHumansImage Processing, Computer-AssistedInfarction, Middle Cerebral ArteryMagnetic Resonance ImagingMaleMesenchymal Stem Cell TransplantationMesenchymal Stem CellsModels, AnimalNeovascularization, PathologicNeuropsychological TestsPlacenta Growth FactorPregnancy ProteinsRatsRats, Sprague-DawleyTransduction, GeneticConceptsMiddle cerebral artery occlusionCerebral ischaemiaMesenchymal stem cellsIntravenous deliveryPermanent middle cerebral artery occlusionHuman MSCsControl sham groupLimb placement testRats 3 hCerebral artery occlusionTreadmill stress testPlacental growth factorIntraluminal vascular occlusionEnzyme-linked immunosorbentNon-neural tissuesHuman mesenchymal stem cellsStem cellsAdult bone marrowArtery occlusionInfarcted hemisphereSham groupFunctional outcomeVascular occlusionFunctional deficitsInfarction size
2005
I.v. infusion of brain-derived neurotrophic factor gene-modified human mesenchymal stem cells protects against injury in a cerebral ischemia model in adult rat
Nomura T, Honmou O, Harada K, Houkin K, Hamada H, Kocsis J. I.v. infusion of brain-derived neurotrophic factor gene-modified human mesenchymal stem cells protects against injury in a cerebral ischemia model in adult rat. Neuroscience 2005, 136: 161-169. PMID: 16229956, PMCID: PMC2605391, DOI: 10.1016/j.neuroscience.2005.06.062.Peer-Reviewed Original ResearchConceptsBrain-derived neurotrophic factorMesenchymal stem cell groupBrain-derived neurotrophic factor contributesMiddle cerebral artery occlusionStem cell groupCerebral artery occlusionCerebral ischemia modelMesenchymal stem cellsNeurotrophic factorArtery occlusionCell groupsIschemia modelPermanent middle cerebral artery occlusionRat cerebral ischemia modelHuman mesenchymal stem cellsBrain-derived neurotrophic factor (BDNF) geneStem cellsControl sham groupTreadmill stress testRats 6 hFactor contributesNeurotrophic factor geneIntraluminal vascular occlusionAdult bone marrowCerebral ischemiaIntravenous infusion of immortalized human mesenchymal stem cells protects against injury in a cerebral ischemia model in adult rat
Honma T, Honmou O, Iihoshi S, Harada K, Houkin K, Hamada H, Kocsis J. Intravenous infusion of immortalized human mesenchymal stem cells protects against injury in a cerebral ischemia model in adult rat. Experimental Neurology 2005, 199: 56-66. PMID: 15967439, PMCID: PMC2605388, DOI: 10.1016/j.expneurol.2005.05.004.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigens, CDBehavior, AnimalBrain IschemiaCell CountCells, CulturedDisease Models, AnimalExercise TestGreen Fluorescent ProteinsHumansImmunohistochemistryInfusions, IntravenousMagnetic Resonance ImagingMagnetic Resonance SpectroscopyMaleMaze LearningMesenchymal Stem Cell TransplantationMesenchymal Stem CellsPhosphopyruvate HydrataseRatsRats, Sprague-DawleyTime FactorsConceptsMiddle cerebral artery occlusionIntravenous infusionFunctional outcomeLesion sizeTransient middle cerebral artery occlusionMesenchymal stem cellsCerebral artery occlusionCerebral infarction volumeCerebral ischemia modelSpinal cord injurySubsequent histological examinationPotential therapeutic benefitRats 12 hMorris water mazeBone marrow cellsHuman mesenchymal stem cellsStem cellsHTERT-MSCsArtery occlusionInfarction volumeCerebral ischemiaCord injuryFunctional improvementLesion volumeIschemia model