2023
Repeated intravenous infusion of mesenchymal stem cells enhances recovery of motor function in a rat model with chronic spinal cord injury
Kurihara K, Sasaki M, Nagahama H, Obara H, Fukushi R, Hirota R, Yoshimoto M, Teramoto A, Kocsis J, Yamashita T, Honmou O. Repeated intravenous infusion of mesenchymal stem cells enhances recovery of motor function in a rat model with chronic spinal cord injury. Brain Research 2023, 1817: 148484. PMID: 37442249, DOI: 10.1016/j.brainres.2023.148484.Peer-Reviewed Original ResearchConceptsSpinal cord injuryInfusion of MSCsChronic spinal cord injuryMesenchymal stem cellsAxonal sproutingCord injuryHD-MSCsIntravenous infusionMotor functionVehicle groupWeek 6Single high-dose infusionInjection of MSCsChronic SCI ratsSingle MSC injectionGreater functional recoveryRat SCI modelSingle intravenous infusionHigh-dose infusionLimited treatment optionsGreater functional improvementSingle high doseHigh disease burdenVivo diffusion tensor imagingEx vivo diffusion tensor imagingHuman mesenchymal stem‐derived extracellular vesicles improve body growth and motor function following severe spinal cord injury in rat
Nakazaki M, Lankford K, Yamamoto H, Mae Y, Kocsis J. Human mesenchymal stem‐derived extracellular vesicles improve body growth and motor function following severe spinal cord injury in rat. Clinical And Translational Medicine 2023, 13: e1284. PMID: 37323108, PMCID: PMC10272923, DOI: 10.1002/ctm2.1284.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCytokinesExtracellular VesiclesHumansInterleukin-6Mesenchymal Stem CellsRatsSpinal Cord InjuriesTumor Necrosis Factor-alphaConceptsSpinal cord injurySevere spinal cord injuryFunctional motor recoveryYoung adult ratsMotor recoveryMesenchymal stem/stromal cellsSmall extracellular vesiclesMSC-sEVsCord injuryM2 macrophagesMotor functionAdult ratsBody growthPro-inflammatory cytokine tumor necrosisAdult spinal cord injuryDay 7 post-SCISystemic pro-inflammatory cytokinesIGF-1 levelsPro-inflammatory cytokinesCytokine tumor necrosisSystemic serum levelsBroad therapeutic benefitsNormal body growthExtracellular vesiclesDifferent treatment groups
2022
Enhanced Network in Corticospinal Tracts after Infused Mesenchymal Stem Cells in Spinal Cord Injury
Hirota R, Sasaki M, Kataoka-Sasaki Y, Oshigiri T, Kurihara K, Fukushi R, Oka S, Ukai R, Yoshimoto M, Kocsis JD, Yamashita T, Honmou O. Enhanced Network in Corticospinal Tracts after Infused Mesenchymal Stem Cells in Spinal Cord Injury. Journal Of Neurotrauma 2022, 39: 1665-1677. PMID: 35611987, PMCID: PMC9734021, DOI: 10.1089/neu.2022.0106.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAxonsMammalsMesenchymal Stem CellsNerve RegenerationPyramidal TractsRecovery of FunctionSpinal CordSpinal Cord InjuriesConceptsSpinal cord injuryCorticospinal tractMesenchymal stem cellsCord injurySpinal cordSpontaneous recoveryInfused mesenchymal stem cellsLimited spontaneous recoveryDorsal corticospinal tractLateral corticospinal tractStem cellsCST pathwayCST projectionsSCI inductionMSC infusionAxonal sproutingFunctional recoveryLateral funiculusIntravenous infusionAxonal tracerLesion coreMotor pathwaysFunctional improvementCircuit reorganizationMajor projections
2001
Transplantation of an acutely isolated bone marrow fraction repairs demyelinated adult rat spinal cord axons
Sasaki M, Honmou O, Akiyama Y, Uede T, Hashi K, Kocsis J. Transplantation of an acutely isolated bone marrow fraction repairs demyelinated adult rat spinal cord axons. Glia 2001, 35: 26-34. PMID: 11424189, PMCID: PMC2605363, DOI: 10.1002/glia.1067.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, NewbornBeta-GalactosidaseBone Marrow TransplantationCells, CulturedEthidiumGlial Fibrillary Acidic ProteinImmunohistochemistryMiceMice, TransgenicMyeloid Progenitor CellsNerve Fibers, MyelinatedNerve RegenerationNeurogliaRatsRats, WistarRecovery of FunctionSpinal CordSpinal Cord InjuriesConceptsBone marrow cellsSpinal cordMyelin-forming cellsMarrow cellsDemyelinated rat spinal cordRat spinal cord axonsDorsal column lesionBone marrow cell fractionRat spinal cordX-irradiation treatmentSpinal cord axonsLacZ transgenic miceSchwann cell myelinationCell fractionCell transplantation techniquesDorsal funiculusPeripheral patternTransgenic miceTransplantation techniquesHematopoietic stem cellsIsolated cell fractionsCordFemoral bonePrecursor cellsTransplantation
2000
Transplantation of olfactory ensheathing cells or Schwann cells restores rapid and secure conduction across the transected spinal cord
Imaizumi T, Lankford K, Kocsis J. Transplantation of olfactory ensheathing cells or Schwann cells restores rapid and secure conduction across the transected spinal cord. Brain Research 2000, 854: 70-78. PMID: 10784108, DOI: 10.1016/s0006-8993(99)02285-4.Peer-Reviewed Original ResearchConceptsRegenerated axonsCell transplantationSpinal cordSchwann cellsTransection siteIsolated spinal cord preparationSpinal cord preparationTransplantation of olfactoryRat spinal cordSpinal cord axonsConduction velocity measurementsTransplantation of cellsCord preparationDorsal columnsAxonal regenerationAxon areaTransplantationImpulse conductionHost tractElectrophysiological recordingsAxonsNormal axonsDonor cellsNeuronal sourcesCord