2023
Repeated intravenous infusion of mesenchymal stem cells enhances recovery of motor function in a rat model with chronic spinal cord injury
Kurihara K, Sasaki M, Nagahama H, Obara H, Fukushi R, Hirota R, Yoshimoto M, Teramoto A, Kocsis J, Yamashita T, Honmou O. Repeated intravenous infusion of mesenchymal stem cells enhances recovery of motor function in a rat model with chronic spinal cord injury. Brain Research 2023, 1817: 148484. PMID: 37442249, DOI: 10.1016/j.brainres.2023.148484.Peer-Reviewed Original ResearchConceptsSpinal cord injuryInfusion of MSCsChronic spinal cord injuryMesenchymal stem cellsAxonal sproutingCord injuryHD-MSCsIntravenous infusionMotor functionVehicle groupWeek 6Single high-dose infusionInjection of MSCsChronic SCI ratsSingle MSC injectionGreater functional recoveryRat SCI modelSingle intravenous infusionHigh-dose infusionLimited treatment optionsGreater functional improvementSingle high doseHigh disease burdenVivo diffusion tensor imagingEx vivo diffusion tensor imaging
2022
Enhanced Network in Corticospinal Tracts after Infused Mesenchymal Stem Cells in Spinal Cord Injury
Hirota R, Sasaki M, Kataoka-Sasaki Y, Oshigiri T, Kurihara K, Fukushi R, Oka S, Ukai R, Yoshimoto M, Kocsis JD, Yamashita T, Honmou O. Enhanced Network in Corticospinal Tracts after Infused Mesenchymal Stem Cells in Spinal Cord Injury. Journal Of Neurotrauma 2022, 39: 1665-1677. PMID: 35611987, PMCID: PMC9734021, DOI: 10.1089/neu.2022.0106.Peer-Reviewed Original ResearchConceptsSpinal cord injuryCorticospinal tractMesenchymal stem cellsCord injurySpinal cordSpontaneous recoveryInfused mesenchymal stem cellsLimited spontaneous recoveryDorsal corticospinal tractLateral corticospinal tractStem cellsCST pathwayCST projectionsSCI inductionMSC infusionAxonal sproutingFunctional recoveryLateral funiculusIntravenous infusionAxonal tracerLesion coreMotor pathwaysFunctional improvementCircuit reorganizationMajor projections
2001
Transplantation of an acutely isolated bone marrow fraction repairs demyelinated adult rat spinal cord axons
Sasaki M, Honmou O, Akiyama Y, Uede T, Hashi K, Kocsis J. Transplantation of an acutely isolated bone marrow fraction repairs demyelinated adult rat spinal cord axons. Glia 2001, 35: 26-34. PMID: 11424189, PMCID: PMC2605363, DOI: 10.1002/glia.1067.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, NewbornBeta-GalactosidaseBone Marrow TransplantationCells, CulturedEthidiumGlial Fibrillary Acidic ProteinImmunohistochemistryMiceMice, TransgenicMyeloid Progenitor CellsNerve Fibers, MyelinatedNerve RegenerationNeurogliaRatsRats, WistarRecovery of FunctionSpinal CordSpinal Cord InjuriesConceptsBone marrow cellsSpinal cordMyelin-forming cellsMarrow cellsDemyelinated rat spinal cordRat spinal cord axonsDorsal column lesionBone marrow cell fractionRat spinal cordX-irradiation treatmentSpinal cord axonsLacZ transgenic miceSchwann cell myelinationCell fractionCell transplantation techniquesDorsal funiculusPeripheral patternTransgenic miceTransplantation techniquesHematopoietic stem cellsIsolated cell fractionsCordFemoral bonePrecursor cellsTransplantationTransplantation of Cryopreserved Adult Human Schwann Cells Enhances Axonal Conduction in Demyelinated Spinal Cord
Kohama I, Lankford K, Preiningerova J, White F, Vollmer T, Kocsis J. Transplantation of Cryopreserved Adult Human Schwann Cells Enhances Axonal Conduction in Demyelinated Spinal Cord. Journal Of Neuroscience 2001, 21: 944-950. PMID: 11157080, PMCID: PMC2605383, DOI: 10.1523/jneurosci.21-03-00944.2001.Peer-Reviewed Original ResearchConceptsHuman Schwann cellsSchwann cellsDorsal columnsSural nerveAxonal conductionIntra-axonal recording techniquesDorsal column lesionLegs of patientsDemyelinated spinal cordHuman sural nerveAdult human Schwann cellsFunctional remyelinationExtensive remyelinationCell-based therapiesMultiple sclerosisVascular diseaseSpinal cordWistar ratsConduction blockAdult CNSConduction velocityLesion zoneAction potentialsMonoclonal antibodiesLesionsTransplantation of Clonal Neural Precursor Cells Derived from Adult Human Brain Establishes Functional Peripheral Myelin in the Rat Spinal Cord
Akiyama Y, Honmou O, Kato T, Uede T, Hashi K, Kocsis J. Transplantation of Clonal Neural Precursor Cells Derived from Adult Human Brain Establishes Functional Peripheral Myelin in the Rat Spinal Cord. Experimental Neurology 2001, 167: 27-39. PMID: 11161590, DOI: 10.1006/exnr.2000.7539.Peer-Reviewed Original ResearchMeSH KeywordsAdultAnimalsBrainBrain Tissue TransplantationCell DifferentiationCells, CulturedCerebral VentriclesClone CellsDemyelinating DiseasesFemaleGenes, ReporterHumansIntermediate Filament ProteinsMaleMiddle AgedMyelin SheathNerve Tissue ProteinsNestinNeural ConductionNeuronsRadiation Injuries, ExperimentalRatsRats, WistarSpinal CordStem Cell TransplantationStem CellsTransplantation, HeterologousConceptsAdult human brainRat spinal cordNestin-positive cellsNeural precursor cellsSpinal cordAdult rat spinal cordNormal conduction velocityHuman brainMitogen withdrawalSpinal cord resultsPrecursor cellsGlia-like cellsSchwann cell myelinationNeural progenitor cellsFunctional remyelinationP0 immunoreactivityRemyelinated axonsExtensive remyelinationCord resultsAnterior hornLateral ventricleSubventricular zoneMajority of cellsMyelin patternSchwann cells
2000
Xenotransplantation of transgenic pig olfactory ensheathing cells promotes axonal regeneration in rat spinal cord
Imaizumi T, Lankford K, Burton W, Fodor W, Kocsis J. Xenotransplantation of transgenic pig olfactory ensheathing cells promotes axonal regeneration in rat spinal cord. Nature Biotechnology 2000, 18: 949-953. PMID: 10973214, PMCID: PMC2605371, DOI: 10.1038/79432.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, Genetically ModifiedAxonsCD59 AntigensCell SeparationElectrophysiologyFlow CytometryFluorescent Antibody Technique, IndirectHumansImmunosuppression TherapyModels, BiologicalOlfactory NerveRatsRats, WistarRegenerationSchwann CellsSciatic NerveSpinal CordSwineTransgenesTransplantation, HeterologousConceptsAxonal regenerationSpinal cordSchwann cellsImpulse conductionLesion-control ratsDorsal column lesionTransplantation of olfactoryRat spinal cordConduction velocity measurementsComplement inhibitory proteinsHyperacute responseRegenerated axonsImmunosuppressed ratsTransection siteLesion sitePeripheral patternHost tractCordNormal axonsDonor cellsAxonsInhibitory proteinRatsDonor cell typeTransgenic pigs[Comparison of myelin-forming cells as candidates for therapeutic transplantation in demyelinated CNS axons].
Imaizumi T, Lankford K, Kocsis J, Sasaki M, Akiyama Y, Hashi K. [Comparison of myelin-forming cells as candidates for therapeutic transplantation in demyelinated CNS axons]. Brain And Nerve 脳と神経 2000, 52: 609-15. PMID: 10934721.Peer-Reviewed Original ResearchConceptsDemyelinated CNS axonsSchwann cellsBrain cellsSpinal cordConduction velocityCNS axonsAdult rat spinal cordFronto-temporal lobesRat spinal cordAdult Schwann cellsDemyelination of axonsMyelin-forming cellsBlock of conductionAdult olfactoryDemyelinated diseasesDemyelinated axonsLess myelinationHistological examinationCNS tissueTherapeutic approachesStimulus trainsDay 28Transplanted OECsAmplitude decrementRemyelinationTransplantation of human olfactory ensheathing cells elicits remyelination of demyelinated rat spinal cord
Kato T, Honmou O, Uede T, Hashi K, Kocsis J. Transplantation of human olfactory ensheathing cells elicits remyelination of demyelinated rat spinal cord. Glia 2000, 30: 209-218. PMID: 10756071, PMCID: PMC2605375, DOI: 10.1002/(sici)1098-1136(200005)30:3<209::aid-glia1>3.0.co;2-8.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCation Transport ProteinsCell SizeCells, CulturedDemyelinating DiseasesDNA ProbesFemaleFungal ProteinsHumansIn Situ HybridizationMembrane Transport ProteinsMicroscopy, ElectronMiddle AgedMyelin SheathNeurogliaOlfactory NerveRatsRats, WistarSaccharomyces cerevisiae ProteinsSpinal CordConceptsSpinal cordDemyelinated rat spinal cordHuman olfactoryImmunosuppressed adult ratsDemyelinated spinal cordAdult mammalian CNSRat spinal cordExtensive remyelinationHuman OECsDemyelinated axonsOlfactory nervePeripheral typeSchwann cellsAdult ratsLesion siteMammalian CNSMyelin sheathRemyelinationCordAxonsSitu hybridizationSimilar numberOlfactoryCellsLarge nuclei
1999
The role of voltage-gated Ca2+ channels in anoxic injury of spinal cord white matter
Imaizumi T, Kocsis J, Waxman S. The role of voltage-gated Ca2+ channels in anoxic injury of spinal cord white matter. Brain Research 1999, 817: 84-92. PMID: 9889329, DOI: 10.1016/s0006-8993(98)01214-1.Peer-Reviewed Original ResearchConceptsVoltage-gated Ca2Spinal cord axonsAnoxic injuryDorsal columnsR-type voltage-gated Ca2N-type calcium channelsSpinal cord white matterRat dorsal columnsDorsal column axonsR-type Ca2Rat spinal cordCord white matterT-type channelsInflux of Ca2Dose-dependent mannerLoss of conductionAxonal conductionSpinal cordChannel blockersCalcium channelsSurface stimulationWhite matterPerfusion solutionInjuryGlass microelectrodes
1998
Transplanted Olfactory Ensheathing Cells Remyelinate and Enhance Axonal Conduction in the Demyelinated Dorsal Columns of the Rat Spinal Cord
Imaizumi T, Lankford K, Waxman S, Greer C, Kocsis J. Transplanted Olfactory Ensheathing Cells Remyelinate and Enhance Axonal Conduction in the Demyelinated Dorsal Columns of the Rat Spinal Cord. Journal Of Neuroscience 1998, 18: 6176-6185. PMID: 9698311, PMCID: PMC2605360, DOI: 10.1523/jneurosci.18-16-06176.1998.Peer-Reviewed Original ResearchConceptsDorsal column axonsRat spinal cordSpinal cordRemyelinated axonsDorsal columnsAdult rat spinal cordExtent of remyelinationTransplantation of OECsSpinal cord lesionsCell injection siteQuantitative histological analysisFunctional remyelinationCord lesionsAxonal conductionNeonatal ratsFocal injectionsConduction blockSchwann cellsConduction velocityInjection siteElectrophysiological propertiesAction potentialsAxonsHistological analysisTransplantation
1992
Conduction properties of spinal cord axons in the myelin-deficient rat mutant
Utzschneider D, Black J, Kocsis J. Conduction properties of spinal cord axons in the myelin-deficient rat mutant. Neuroscience 1992, 49: 221-228. PMID: 1407548, DOI: 10.1016/0306-4522(92)90090-o.Peer-Reviewed Original ResearchConceptsDorsal column axonsMyelin-deficient rat mutantsSpinal cordFrequency-dependent conduction blockNormal age-matched ratsBrain slice chamberMyelin-deficient rat spinal cordAction potential dischargeRat mutantAge-matched ratsRat spinal cordSpinal cord axonsAction potential conductionControl ratsDemyelinated axonsConduction blockConduction velocityRefractory periodPotential dischargeControl axonsSlice chamberPharmacological resultsPotential conductionAxonsCompensatory mechanisms
1991
Differential sensitivity to hypoxia of the peripheral versus central trajectory of primary afferent axons
Utzschneider D, Kocsis J, Waxman S. Differential sensitivity to hypoxia of the peripheral versus central trajectory of primary afferent axons. Brain Research 1991, 551: 136-141. PMID: 1913145, DOI: 10.1016/0006-8993(91)90924-k.Peer-Reviewed Original ResearchConceptsDorsal columnsDorsal rootsAfferent fibersCentral nervous system componentsPrimary afferent fibersSucrose gap chamberAction potential amplitudePrimary afferent axonsCompound action potentialDorsal spinal rootsNervous system componentsAxonal trunksPeripheral nervesSpinal cordSpinal rootsAfferent axonsCNS portionSchwann cellsAdult ratsPotential amplitudeAxon branchesAction potentialsHypoxiaMembrane potential changesMembrane depolarization
1985
Aminopyridine-sensitivity of spinal cord white matter studied in vitro
Kocsis J. Aminopyridine-sensitivity of spinal cord white matter studied in vitro. Experimental Brain Research 1985, 57: 620-624. PMID: 2984039, DOI: 10.1007/bf00237849.Peer-Reviewed Original ResearchMeSH Keywords4-AminopyridineAction PotentialsAminopyridinesAnimalsIn Vitro TechniquesRatsRats, Inbred StrainsSpinal CordConceptsCentral nervous system extensionSpinal cord white matterAction potential dischargeDorsal column axonsSucrose gap chamberCompound action potentialPeripheral nervous systemCord white matterVentral root fibersAction potential characteristicsSensory fibersDorsal columnsDorsal rootsSpinal cordNervous systemWhite matterAction potentialsPotential dischargeRoot fibersPotassium channelsCourse of maturationOcclusion experimentsAxonsPronounced increaseCord
1982
Intra-axonal recordings in rat dorsal column axons: membrane hyperpolarization and decreased excitability precede the primary afferent depolarization
Kocsis J, Waxman S. Intra-axonal recordings in rat dorsal column axons: membrane hyperpolarization and decreased excitability precede the primary afferent depolarization. Brain Research 1982, 238: 222-227. PMID: 6282392, DOI: 10.1016/0006-8993(82)90787-9.Peer-Reviewed Original ResearchConceptsPrimary afferent depolarizationIntra-axonal recordingsRat lumbosacral spinal cordDorsal column stimulationDorsal root potentialsLumbosacral spinal cordDorsal column axonsDorsal columnsDorsal rootsAxonal depolarizationRoot potentialsSpinal cordGABA responsesNeuronal elementsIntracellular correlatesProlonged depolarizationMembrane hyperpolarizationHyperpolarizationDepolarizationExcitability
1980
Absence of potassium conductance in central myelinated axons
Kocsis J, Waxman S. Absence of potassium conductance in central myelinated axons. Nature 1980, 287: 348-349. PMID: 7421994, DOI: 10.1038/287348a0.Peer-Reviewed Original ResearchConceptsCentral myelinated axonsMyelinated axonsAction potentialsPotassium conductanceDorsal column axonsVoltage-clamp experimentsLate outward currentOutward currentsAxonsSodium ion permeabilityLate increaseDepolarization phasePotassium permeabilityAxonal membraneRepolarizationMyelinInitial increaseVoltage-dependent changesSodium inactivationDemyelinationPrevious studies