Preparation of reduced representation bisulfite sequencing libraries for genome-scale DNA methylation profiling
Gu H, Smith ZD, Bock C, Boyle P, Gnirke A, Meissner A. Preparation of reduced representation bisulfite sequencing libraries for genome-scale DNA methylation profiling. Nature Protocols 2011, 6: 468-481. PMID: 21412275, DOI: 10.1038/nprot.2010.190.Peer-Reviewed Original ResearchMeSH KeywordsCpG IslandsDNA MethylationGene LibraryGenomicsPolymerase Chain ReactionSequence Analysis, DNASulfitesConceptsSingle-nucleotide resolutionGenome-scale DNA methylation profilingCpG-rich DNA fragmentsGenome-wide mappingGenome-wide scaleStandard molecular biology laboratoryMajority of promotersRelevant genomic regionsIllumina Genome AnalyzerNext-generation sequencing approachDNA methylation profilingDNA methylation measurementsRestriction enzyme MspIReduced representationMolecular biology laboratoryGenomic regionsRRBS librariesGenome AnalyzerSequencing librariesMethylation profilingSequencing approachEnzyme MspIAmount of sequencingLow input requirementsGenomic DNA