2021
Detection of differentially abundant cell subpopulations in scRNA-seq data
Zhao J, Jaffe A, Li H, Lindenbaum O, Sefik E, Jackson R, Cheng X, Flavell RA, Kluger Y. Detection of differentially abundant cell subpopulations in scRNA-seq data. Proceedings Of The National Academy Of Sciences Of The United States Of America 2021, 118: e2100293118. PMID: 34001664, PMCID: PMC8179149, DOI: 10.1073/pnas.2100293118.Peer-Reviewed Original ResearchMeSH KeywordsAgingB-LymphocytesBrainCell LineageCOVID-19CytokinesDatasets as TopicDendritic CellsGene Expression ProfilingGene Expression RegulationHigh-Throughput Nucleotide SequencingHumansMelanomaMonocytesPhenotypeRNA, Small CytoplasmicSARS-CoV-2Severity of Illness IndexSingle-Cell AnalysisSkin NeoplasmsT-LymphocytesTranscriptomeConceptsDA subpopulationsIll COVID-19 patientsImmune checkpoint therapyCOVID-19 patientsSingle-cell RNA sequencing analysisCheckpoint therapyBrain tissueCell subpopulationsRNA sequencing analysisTime pointsSubpopulationsDiseased individualsDistinct phenotypesOriginal studyCell typesAbundant subpopulationSequencing analysisCellsDA measuresPhenotypeImportant differencesNonrespondersPatientsTherapy
2017
Ritornello: high fidelity control-free chromatin immunoprecipitation peak calling
Stanton KP, Jin J, Lederman RR, Weissman SM, Kluger Y. Ritornello: high fidelity control-free chromatin immunoprecipitation peak calling. Nucleic Acids Research 2017, 45: e173-e173. PMID: 28981893, PMCID: PMC5716106, DOI: 10.1093/nar/gkx799.Peer-Reviewed Original ResearchMeSH KeywordsAlgorithmsArtifactsBinding SitesChromatin ImmunoprecipitationDNAHigh-Throughput Nucleotide SequencingNucleotide MotifsSequence Analysis, DNASoftwareTranscription FactorsConceptsChIP-seqNext-generation high-throughput DNA sequencing technologiesHigh-throughput DNA sequencing technologiesGenome-wide localizationGenome-wide scaleTF-binding sitesTranscription factor bindingDNA sequencing technologiesENCODE consortiumResultant readsChromatin immunoprecipitationReference genomeDiverse biological effectsModification eventsFactor bindingOmics techniquesSequencing technologiesOmics experimentsSequencing costsWide localizationPeak callingChip targetArtifactual peaksPeak callersBiological effects