2023
Biochemical functions and structure of Caenorhabditis elegans ZK177.8 protein: Aicardi–Goutières syndrome SAMHD1 dNTPase ortholog
Maehigashi T, Lim C, Wade L, Bowen N, Knecht K, Alvarez N, Kelly W, Schinazi R, Kim D, Xiong Y, Kim B. Biochemical functions and structure of Caenorhabditis elegans ZK177.8 protein: Aicardi–Goutières syndrome SAMHD1 dNTPase ortholog. Journal Of Biological Chemistry 2023, 299: 105148. PMID: 37567474, PMCID: PMC10485159, DOI: 10.1016/j.jbc.2023.105148.Peer-Reviewed Original ResearchSterile alpha motif (SAM) domainMotif domainHistidine-aspartate domain-containing protein 1Allosteric regulatory mechanismsDomain-containing protein 1Cellular dNTP levelsStriking conservationCaenorhabditis elegansBiochemical functionsDevelopmental roleGene knockdownRegulatory mechanismsDevelopmental defectsHuman monocytic cell lineDNTP levelsMonocytic cell lineMouse SAMHD1Aicardi-Goutières syndromeProtein 1OrthologsHIV-1 restriction factorsProteinCell linesRestriction factorsDNA building blocks
2014
Kill HIV by Starvation–Mechanism of Allosteric Activation of SAMHD1
Ji X, Wu Y, Yan J, Mehrens J, DeLucia M, Hao C, Gronenborn A, Skowronski J, Anh J, Xiong Y. Kill HIV by Starvation–Mechanism of Allosteric Activation of SAMHD1. Acta Crystallographica Section A: Foundations And Advances 2014, 70: c121-c121. DOI: 10.1107/s2053273314098787.Peer-Reviewed Original ResearchCellular dNTP poolsCellular dNTP levelsDNTP poolsDNTP levelsDeoxyribonucleoside triphosphate triphosphohydrolaseActive tetrameric formMammalian cellsAllosteric activationSAMHD1 functionAllosteric mechanismMajor regulatorAllosteric siteInactive dimerAllosteric bindingTetrameric formAicardi-Goutières syndromeTetrameric complexMechanistic understandingCongenital viral infectionViral reverse transcriptionMonomers/dimersSAMHD1Reverse transcriptionHIV restrictionClinical manifestations