2020
Contractile work directly modulates mitochondrial protein levels in human engineered heart tissues
Ng R, Sewanan LR, Brill AL, Stankey P, Li X, Qyang Y, Ehrlich BE, Campbell SG. Contractile work directly modulates mitochondrial protein levels in human engineered heart tissues. AJP Heart And Circulatory Physiology 2020, 318: h1516-h1524. PMID: 32383992, PMCID: PMC7311697, DOI: 10.1152/ajpheart.00055.2020.Peer-Reviewed Original ResearchConceptsMitochondrial proteinsMitochondrial massMitochondrial protein levelsMitochondrial protein expressionMyosin ATPase inhibitorMitochondrial biogenesisExtended tissue culturePathological cardiac adaptationATP demandActive length controlFull recapitulationBiogenesisLength controlHeart tissueProtein levelsATPase inhibitorProtein expressionCulture environmentProteinTissue cultureCardiac physiologyNovel bioreactorVivo conditionsContractile workVitro method
2011
Methods of Cell Purification: A Critical Juncture for Laboratory Research and Translational Science
Amos PJ, Bozkulak E, Qyang Y. Methods of Cell Purification: A Critical Juncture for Laboratory Research and Translational Science. Cells Tissues Organs 2011, 195: 26-40. PMID: 21996576, PMCID: PMC3257814, DOI: 10.1159/000331390.Peer-Reviewed Original ResearchConceptsCell purificationSpecific cell populationsPhenotypic traitsCell biologySurface proteinsGenetic expressionCellular reagentsSpecific cellsCell populationsSorting cellsCell separation strategiesPurificationCellsHeterogeneous mixtureAbility of researchersBiologyTraitsProteinCertain mechanismsExpression
2001
Bob1, a Gim5/MM-1/Pfd5 homolog, interacts with the MAP kinase kinase Byr1 to regulate sexual differentiation in the fission yeast, Schizosaccharomyces pombe
Henkel J, Du H, Yang P, Qyang Y, Kansra S, Ko M, Kim H, Marcus S. Bob1, a Gim5/MM-1/Pfd5 homolog, interacts with the MAP kinase kinase Byr1 to regulate sexual differentiation in the fission yeast, Schizosaccharomyces pombe. Differentiation 2001, 67: 98-106. PMID: 11683500, DOI: 10.1046/j.1432-0436.2001.670402.x.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceBase SequenceCatalysisCell DifferentiationConserved SequenceCytoskeletonHumansMicroscopy, FluorescenceMolecular Sequence DataMutationProtein BindingProtein KinasesRepressor ProteinsReproduction, AsexualSchizosaccharomycesSchizosaccharomyces pombe ProteinsSubstrate SpecificityThiabendazoleTwo-Hybrid System TechniquesConceptsS. pombe cellsFission yeastPombe cellsObvious growth defectTwo-hybrid screenRegulators of actinSexual differentiationInhibition of matingMating deficiencyEukaryotic organismsMAPK moduleNull mutantsS. pombeCytoskeletal controlGrowth defectCytoskeletal defectsMating responseByr1Diverse rolesMutantsMolecular characterizationYeastProteinSchizosaccharomycesOverexpression