2005
High Spatial Resolution Observation of Single-Molecule Dynamics in Living Cell Membranes
Edel J, Wu M, Baird B, Craighead H. High Spatial Resolution Observation of Single-Molecule Dynamics in Living Cell Membranes. Biophysical Journal 2005, 88: l43-l45. PMID: 15821167, PMCID: PMC1305672, DOI: 10.1529/biophysj.105.061937.Peer-Reviewed Original ResearchConceptsZero-mode waveguidesHigh spatial resolution observationsSpatial resolution observationsCell membraneSingle-molecule dynamicsOptical confinementBiological membrane functionResolution observationsCell membrane componentsFluorescent excitationCellular organellesCytosolic poolCellular membranesMembrane componentsSingle moleculesMembrane functionLipid diffusionBiological membranesRat basophilic leukemia cellsLipid bilayersConfocal microscopyConfinementBasophilic leukemia cellsIndividual moleculesEssential building blocks
2004
Visualization of plasma membrane compartmentalization with patterned lipid bilayers
Wu M, Holowka D, Craighead HG, Baird B. Visualization of plasma membrane compartmentalization with patterned lipid bilayers. Proceedings Of The National Academy Of Sciences Of The United States Of America 2004, 101: 13798-13803. PMID: 15356342, PMCID: PMC518836, DOI: 10.1073/pnas.0403835101.Peer-Reviewed Original ResearchConceptsLipid raftsLeaflet componentsPlasma membrane compartmentalizationReceptor clustersMembrane structural organizationLipid bilayersFcepsilon receptor ITyrosine phosphorylation activityMembrane compartmentalizationLyn kinaseInner leafletPhosphorylation activityActin polymerizationCellular componentsStructural organizationCell receptorRaftsCell activationReceptor IMast cell receptorsStructural reorganizationKinaseUnique insightsBilayersProtein