1999
Inhibition of neurotransmitter release in the lamprey reticulospinal synapse by antibody-mediated disruption of SNAP-25 function
Low P, Norlin T, Risinger C, Larhammar D, Pieribone V, Shupliakov O, Brodin L. Inhibition of neurotransmitter release in the lamprey reticulospinal synapse by antibody-mediated disruption of SNAP-25 function. European Journal Of Cell Biology 1999, 78: 787-793. PMID: 10604655, DOI: 10.1016/s0171-9335(99)80029-6.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodies, MonoclonalAxonsCell MembraneImmunohistochemistryIn Situ HybridizationLampreysMembrane ProteinsMicroinjectionsMicroscopy, ElectronNerve Tissue ProteinsNeurotransmitter AgentsProtein BindingRecombinant Fusion ProteinsSpinal CordSynapsesSynaptic TransmissionSynaptic VesiclesSynaptosomal-Associated Protein 25ConceptsPlasma membraneSynaptic vesiclesSNAP-25Lamprey giant reticulospinal synapsePresynaptic plasma membraneSynaptic vesicle exocytosisLamprey reticulospinal synapseSNARE protein SNAP-25Protein SNAP-25Synaptic vesicle clustersNeurotransmitter releaseSNARE complexMolecular machineryVesicle exocytosisMembrane fusionVesicle cyclingPresynaptic microinjectionVesicle clustersSyntaxinPresynaptic membraneVesiclesElectron microscopic analysisAntibody-mediated disruptionMembraneMicroscopic analysis
1995
Distinct pools of synaptic vesicles in neurotransmitter release
Pieribone V, Shupliakov O, Brodin L, Hilfiker-Rothenfluh S, Czernik A, Greengard P. Distinct pools of synaptic vesicles in neurotransmitter release. Nature 1995, 375: 493-497. PMID: 7777058, DOI: 10.1038/375493a0.Peer-Reviewed Original ResearchConceptsNeurotransmitter releaseCellular secretory systemSynaptic vesiclesLow-frequency stimulationSynaptic release sitesRelease of neurotransmittersHigh-frequency burstsNeuron-specific proteinSynapsin antibodiesPool of vesiclesDistal poolNerve terminalsMarked depressionVesicular releasePresynaptic membraneSynapsinHigh rateClusters of vesiclesApparent effectReleaseSecretory systemSuch protein familiesNeurotransmittersRelease sitesSynaptic Vesicle Depletion in Reticulospinal Axons is Reduced by 5‐hydroxytryptamine: Direct Evidence for Presynaptic Modulation of Glutamatergic Transmission
Shupliakov O, Pieribone VA, Gad H, Brodin L. Synaptic Vesicle Depletion in Reticulospinal Axons is Reduced by 5‐hydroxytryptamine: Direct Evidence for Presynaptic Modulation of Glutamatergic Transmission. European Journal Of Neuroscience 1995, 7: 1111-1116. PMID: 7613617, DOI: 10.1111/j.1460-9568.1995.tb01099.x.Peer-Reviewed Original ResearchConceptsReticulospinal axonsPresynaptic Ca2Glutamatergic synaptic transmissionSynaptic glutamate releaseSynaptic vesiclesIntra-axonal recordingsGiant reticulospinal axonsSynaptic vesicle clustersGlutamatergic transmissionPresynaptic modulationGlutamate releaseSpinal cordPresynaptic elementsSynaptic transmissionAction potential stimulationSynaptic vesicle depletionSame axonVesicle depletionAxonsMarked reductionNumber of vesiclesPotential stimulationSynaptic exocytosisActive zoneCa2
1994
The reticulospinal glutamate synapse in lamprey: plasticity and presynaptic variability
Brodin L, Shupliakov O, Pieribone VA, Hellgren J, Hill RH. The reticulospinal glutamate synapse in lamprey: plasticity and presynaptic variability. Journal Of Neurophysiology 1994, 72: 592-604. PMID: 7983521, DOI: 10.1152/jn.1994.72.2.592.Peer-Reviewed Original ResearchConceptsExcitatory postsynaptic potentialsReticulospinal axonsAction potentialsPulse stimulationSynaptic areaBody stimulationElectrotonic componentUse-dependent modulationAxonal action potentialsSecond action potentialDifferent postsynaptic cellsGiant reticulospinal axonsNet facilitationSpinal neuronsPostsynaptic potentialsEPSP componentsGlutamatergic synapsesPresynaptic elementsSynaptic responsesPresynaptic axonsTransmitter releaseGlutamate synapsePostsynaptic cellDegree of facilitationDirect stimulation