2017
10 Single cell expression quantitative trait LOCI (EQTL) analsis of established lupus-risk loci in patient monocytes
Ghodke-Puranik Y, Zhongbo J, Fan W, Jensen M, Dorschner J, Vsetecka D, Amin S, Makol A, Ernste F, Osborn T, Moder K, Chowdhary V, Niewold T. 10 Single cell expression quantitative trait LOCI (EQTL) analsis of established lupus-risk loci in patient monocytes. Lupus Science & Medicine 2017, 4: a6. DOI: 10.1136/lupus-2017-000215.10.Peer-Reviewed Original ResearchCell typesFluidigm C1 systemDifferent cellular eventsRelated cell typesSLE risk lociDifferent cell typesEQTL associationsLoci interactEQTL analysisGene expressionMajor unanswered questionSingle-cell captureCellular eventsLoci influenceSame SNPLociPleiotropic effectsGenesEQTLsSPP1SNPsC1 systemLimited overlapImmune cell typesImmune system function
2016
GG-06 Single cell gene expression studies in lupus patient monocytes reveal novel patterns reflecting disease activity, interferon, and medical treatment
Jin Z, Fan W, Jensen M, Dorschner J, Vsetecka D, Amin S, Makol A, Ernste F, Osborn T, Moder K, Chowdhary V, Niewold T. GG-06 Single cell gene expression studies in lupus patient monocytes reveal novel patterns reflecting disease activity, interferon, and medical treatment. Lupus Science & Medicine 2016, 3: a30. DOI: 10.1136/lupus-2016-000179.58.Peer-Reviewed Original ResearchNon-classical monocytesSLE patientsDisease activityClassical monocytesPatients' monocytesCell gene expressionAnti-dsDNA titersHigh disease activityHigher IFN scoresSLE patient groupAnti-inflammatory genesMonocyte gene expressionPrednisone useGene expressionMedication useClinical featuresInterferon signatureC3 complementIFN scorePatient groupImmunologic characteristicsUnsupervised hierarchical clusteringMedical treatmentPatients