O-Glycosylation of a Secretory Granule Membrane Enzyme Is Essential for Its Endocytic Trafficking*
Vishwanatha KS, Bäck N, Lam TT, Mains RE, Eipper BA. O-Glycosylation of a Secretory Granule Membrane Enzyme Is Essential for Its Endocytic Trafficking*. Journal Of Biological Chemistry 2016, 291: 9835-9850. PMID: 26961877, PMCID: PMC4850319, DOI: 10.1074/jbc.m115.711838.Peer-Reviewed Original ResearchConceptsHigh molecular weight complexesPAM-1Molecular weight complexesEndocytic traffickingCytosolic domainBlue native PAGE analysisAtT-20 corticotrope tumor cellsWeight complexesCrucial post-translational modificationPost-translational modificationsO-glycosylation sitesPeptidylglycine αFurin-like convertasesNative PAGE analysisSoluble fragmentCorticotrope tumor cellsAlternative splicingEndocytic pathwayCatalytic domainEndocytic compartmentsGlycosylation sitesO-glycosylationMultivesicular bodiesMembrane enzymeEndoproteolytic cleavage