2015
A single-nucleotide-polymorphism real-time PCR assay for genotyping of Mycobacterium tuberculosis complex in peri-urban Kampala
Wampande EM, Hatzios SK, Achan B, Mupere E, Nsereko M, Mayanja HK, Eisenach K, Boom WH, Gagneux S, Joloba ML, For the Tuberculosis Research Unit. A single-nucleotide-polymorphism real-time PCR assay for genotyping of Mycobacterium tuberculosis complex in peri-urban Kampala. BMC Infectious Diseases 2015, 15: 396. PMID: 26423522, PMCID: PMC4590274, DOI: 10.1186/s12879-015-1121-7.Peer-Reviewed Original ResearchConceptsMycobacterium tuberculosis complexSputum samplesTB patientsReal-time PCRSingle nucleotide polymorphismsLineage-specific single nucleotide polymorphismsSmear-positive sputum samplesMTBC strainsTuberculosis complexLineage 4Pathogenesis of tuberculosisMTBC lineagesLineage 3Indeterminate lineagePeri-urban KampalaLineages/sublineagesMTBC isolatesHigh-throughput genotypingTargeted sequencingPatientsHigh-throughput techniquesTuberculosisFurther validationRoutine genotypingNucleotide polymorphisms
2013
Osmosensory signaling in Mycobacterium tuberculosis mediated by a eukaryotic-like Ser/Thr protein kinase
Hatzios SK, Baer CE, Rustad TR, Siegrist MS, Pang JM, Ortega C, Alber T, Grundner C, Sherman DR, Bertozzi CR. Osmosensory signaling in Mycobacterium tuberculosis mediated by a eukaryotic-like Ser/Thr protein kinase. Proceedings Of The National Academy Of Sciences Of The United States Of America 2013, 110: e5069-e5077. PMID: 24309377, PMCID: PMC3876250, DOI: 10.1073/pnas.1321205110.Peer-Reviewed Original ResearchConceptsEukaryotic-like Ser/Thr protein kinasesSer/Thr protein kinaseOsmotic stressProtein kinaseCell wall remodelingAlternative σ factorsVirulence factor productionΣ factorsTranscriptional programsSecretion systemESX-1 secretion systemBroader roleWall remodelingWidespread occurrenceKinaseBacteriaPhysiological changesFactor productionPathwayDifferent microenvironmentsAntibiotic resistanceOsmosensingRegulonPknDMycobacterium tuberculosis
2011
The Regulation of Sulfur Metabolism in Mycobacterium tuberculosis
Hatzios SK, Bertozzi CR. The Regulation of Sulfur Metabolism in Mycobacterium tuberculosis. PLOS Pathogens 2011, 7: e1002036. PMID: 21811406, PMCID: PMC3141025, DOI: 10.1371/journal.ppat.1002036.Peer-Reviewed Original ResearchMeSH KeywordsHost-Pathogen InteractionsHumansMacrophages, AlveolarMycobacterium tuberculosisSulfatesSulfurTuberculosisConceptsSulfate assimilation pathwayAssimilation pathwayDiverse environmental cuesMycobacterium tuberculosisBiosynthesis of moleculesHost immune cellsSmall molecule regulatorsSuccessful human pathogenSulfur metabolismRegulatory cuesMtb infectionGranuloma formationImmune cellsEnvironmental cuesRegulatory proteinsSulfur-containing metabolitesMolecule regulatorsAlveolar macrophagesTherapeutic interventionsMetabolism regulationBiochemical mechanismsIntracellular pathogensMetabolic adaptationHuman pathogensTuberculosis