2014
Autotransporters but not pAA are critical for rabbit colonization by Shiga toxin-producing Escherichia coli O104:H4
Munera D, Ritchie JM, Hatzios SK, Bronson R, Fang G, Schadt EE, Davis BM, Waldor MK. Autotransporters but not pAA are critical for rabbit colonization by Shiga toxin-producing Escherichia coli O104:H4. Nature Communications 2014, 5: 3080. PMID: 24445323, PMCID: PMC3905246, DOI: 10.1038/ncomms4080.Peer-Reviewed Original ResearchConceptsEnteroaggregative Escherichia coliIntestinal colonizationAggregative adherenceHaemolytic uraemic syndromeShiga toxin-producing Escherichia coli O104Outbreak of diarrheaIntestinal pathologyUraemic syndromeEscherichia coli O104Diarrhoeal diseaseOutbreak strainShiga toxinDiarrheaColi O104Subset of strainsAdherenceRobust colonizationCultured cellsCharacteristic patternVirulenceSyndromeDiseasePathologyColonization
2012
Studies of Dynamic Protein-Protein Interactions in Bacteria Using Renilla Luciferase Complementation Are Undermined by Nonspecific Enzyme Inhibition
Hatzios SK, Ringgaard S, Davis BM, Waldor MK. Studies of Dynamic Protein-Protein Interactions in Bacteria Using Renilla Luciferase Complementation Are Undermined by Nonspecific Enzyme Inhibition. PLOS ONE 2012, 7: e43175. PMID: 22905225, PMCID: PMC3419657, DOI: 10.1371/journal.pone.0043175.Peer-Reviewed Original ResearchConceptsDynamic protein-protein interactionsProtein-protein interactionsLuciferase complementationFull-length luciferaseLive eukaryotic cellsEnteric pathogen Vibrio choleraeProtein fragment complementationSmall molecule effectsPathogen Vibrio choleraeEnzyme activityPhosphatase CheZEukaryotic cellsFragment complementationChemotaxis mutantsLuciferase fragmentsChemotactic signalingBacterial chemotaxisFusion proteinComplementationRenilla luciferaseLuciferase activityCheY3Vibrio choleraeProteinLuciferase