Studies of Dynamic Protein-Protein Interactions in Bacteria Using Renilla Luciferase Complementation Are Undermined by Nonspecific Enzyme Inhibition
Hatzios SK, Ringgaard S, Davis BM, Waldor MK. Studies of Dynamic Protein-Protein Interactions in Bacteria Using Renilla Luciferase Complementation Are Undermined by Nonspecific Enzyme Inhibition. PLOS ONE 2012, 7: e43175. PMID: 22905225, PMCID: PMC3419657, DOI: 10.1371/journal.pone.0043175.Peer-Reviewed Original ResearchConceptsDynamic protein-protein interactionsProtein-protein interactionsLuciferase complementationFull-length luciferaseLive eukaryotic cellsEnteric pathogen Vibrio choleraeProtein fragment complementationSmall molecule effectsPathogen Vibrio choleraeEnzyme activityPhosphatase CheZEukaryotic cellsFragment complementationChemotaxis mutantsLuciferase fragmentsChemotactic signalingBacterial chemotaxisFusion proteinComplementationRenilla luciferaseLuciferase activityCheY3Vibrio choleraeProteinLuciferase