2020
ProbeDealer is a convenient tool for designing probes for highly multiplexed fluorescence in situ hybridization
Hu M, Yang B, Cheng Y, Radda JSD, Chen Y, Liu M, Wang S. ProbeDealer is a convenient tool for designing probes for highly multiplexed fluorescence in situ hybridization. Scientific Reports 2020, 10: 22031. PMID: 33328483, PMCID: PMC7745008, DOI: 10.1038/s41598-020-76439-x.Peer-Reviewed Original ResearchConceptsSingle-molecule RNA FISHSpecific genomic lociSitu hybridizationNucleome architecturesRNA FISHGenomic lociRNA speciesMultiplexed fluorescenceFISH techniqueFishRecent technological advancesMultiplexed imagingHybridizationMultiplexed mannerLociSpeciesFluorescencePowerful methodNotable exampleProbeProbe designTechnological advances
2016
An RNA-aptamer-based two-color CRISPR labeling system
Wang S, Su JH, Zhang F, Zhuang X. An RNA-aptamer-based two-color CRISPR labeling system. Scientific Reports 2016, 6: 26857. PMID: 27229896, PMCID: PMC4882555, DOI: 10.1038/srep26857.Peer-Reviewed Original ResearchConceptsSingle guide RNAsDifferent fluorescent proteinsGenomic lociFluorescent proteinSpecific chromatin lociEndogenous genomic lociDynamics of chromatinPP7 coat proteinTarget genomic lociStem loop 2Essential biological functionsSpecific DNA sequencesDifferent bacterial speciesChromatin imagingCRISPR single guide RNAsChromatin lociRepetitive sequencesDNA sequencesChromatin labelingCoat proteinBiological functionsHuman cellsLoop 2RNA aptamersBacterial species