2022
High-content CRISPR screening
Bock C, Datlinger P, Chardon F, Coelho M, Dong M, Lawson K, Lu T, Maroc L, Norman T, Song B, Stanley G, Chen S, Garnett M, Li W, Moffat J, Qi L, Shapiro R, Shendure J, Weissman J, Zhuang X. High-content CRISPR screening. Nature Reviews Methods Primers 2022, 2: 8. DOI: 10.1038/s43586-021-00093-4.Peer-Reviewed Original ResearchCRISPR screeningCRISPR screensBiological discoverySingle-cell RNA sequencingPooled CRISPR screensList of genesHigh-content methodBiological challengesGene functionCell competitionUnbiased interrogationGuide RNARNA sequencingBioinformatics analysisDetailed biological insightsTarget genesBasic biologyPool of cellsBiological insightsCRISPR technologyMolecular mechanismsSuch screensGenesMedical GeneticsBroad utility
2018
Programmable DNA repair with CRISPRa/i enhanced homology-directed repair efficiency with a single Cas9
Ye L, Wang C, Hong L, Sun N, Chen D, Chen S, Han F. Programmable DNA repair with CRISPRa/i enhanced homology-directed repair efficiency with a single Cas9. Cell Discovery 2018, 4: 46. PMID: 30062046, PMCID: PMC6056518, DOI: 10.1038/s41421-018-0049-7.Peer-Reviewed Original ResearchHomology-directed repairDNA double-strand breaksCRISPRa/iGene editingDNA repairHDR efficiencySite-specific genome engineeringPrecise genome modificationNon-homologous DNA endDNA repair factorsRNA interference-mediated inhibitionDNA repair pathwaysDouble-strand breaksGene editing processHomology-directed repair efficiencyPrecise gene editingGenome modificationGenome engineeringRepair factorsMammalian cellsHDR pathwayNHEJ factorsDNA endsGuide RNAHDR rates