2008
An Uncharged Amine in the Transition State of the Ribosomal Peptidyl Transfer Reaction
Kingery DA, Pfund E, Voorhees RM, Okuda K, Wohlgemuth I, Kitchen DE, Rodnina MV, Strobel SA. An Uncharged Amine in the Transition State of the Ribosomal Peptidyl Transfer Reaction. Cell Chemical Biology 2008, 15: 493-500. PMID: 18482701, PMCID: PMC2851197, DOI: 10.1016/j.chembiol.2008.04.005.Peer-Reviewed Original ResearchConceptsTransition stateAminolysis reactionBond formationNitrogen-carbon bond formationLinear free energy relationshipSubstantial positive chargeChemical transition stateFree energy relationshipsPeptidyl transfer reactionTransfer reactionsActive sitePositive chargeUncharged amineReactionNucleophilesPuromycin derivativesDeprotonationChemistryDetailed understandingAminesFormationDerivativesChargeSolution
2004
Synthesis of isotopically labeled puromycin derivatives for kinetic isotope effect analysis of ribosome catalyzed peptide bond formation
Okuda K, Seila A, Strobel S. Synthesis of isotopically labeled puromycin derivatives for kinetic isotope effect analysis of ribosome catalyzed peptide bond formation. Tetrahedron 2004, 60: 12101-12112. DOI: 10.1016/j.tet.2004.10.023.Peer-Reviewed Original Research