2009
Detection of Leishmania in Unaffected Mucosal Tissues of Patients with Cutaneous Leishmaniasis Caused by Leishmania (Viannia) Species
Figueroa RA, Lozano LE, Romero IC, Cardona MT, Prager M, Pacheco R, Diaz YR, Tellez JA, Saravia NG. Detection of Leishmania in Unaffected Mucosal Tissues of Patients with Cutaneous Leishmaniasis Caused by Leishmania (Viannia) Species. The Journal Of Infectious Diseases 2009, 200: 638-646. PMID: 19569974, PMCID: PMC5056532, DOI: 10.1086/600109.Peer-Reviewed Original ResearchConceptsKinetoplast minicircle DNAMucosal tissuesCutaneous leishmaniasisMucocutaneous leishmaniasisNasal mucosaSwab samplesMucosa of patientsLeishmania speciesPresence of LeishmaniaDetection of LeishmaniaMucosal leishmaniasisMucosal diseaseCutaneous diseaseLeishmania infectionPolymerase chain reactionParasitological evaluationPatientsLeishmania panamensisLeishmania guyanensisMucosaNatural historyLeishmaniasisLeishmania braziliensisChain reactionAsymptomatic presence
2005
Amplification of human DNA by primers targeted to Leishmania kinetoplast DNA and post-genome considerations in the detection of parasites by a polymerase chain reaction.
Vergel C, Walker J, SARAVIA NG. Amplification of human DNA by primers targeted to Leishmania kinetoplast DNA and post-genome considerations in the detection of parasites by a polymerase chain reaction. American Journal Of Tropical Medicine And Hygiene 2005, 72: 423-9. PMID: 15827280, DOI: 10.4269/ajtmh.2005.72.423.Peer-Reviewed Original ResearchConceptsMolecular amplification techniquesPerformance of primersPolymerase chain reaction productsAmplification techniquesMonocyte DNADNA probesLeishmania kinetoplast DNANew applicationsChain reaction productsDNA amplificationHuman DNA sequencesDNA sequencesDetection of parasitesPolymerase chain reaction
2000
Evaluation of Polymerase Chain Reaction, Adenosine Deaminase, and Interferon-γ in Pleural Fluid for the Differential Diagnosis of Pleural Tuberculosis
Villegas M, Labrada L, Saravia N. Evaluation of Polymerase Chain Reaction, Adenosine Deaminase, and Interferon-γ in Pleural Fluid for the Differential Diagnosis of Pleural Tuberculosis. CHEST Journal 2000, 118: 1355-1364. PMID: 11083686, DOI: 10.1378/chest.118.5.1355.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine DeaminaseAge FactorsBacteriological TechniquesBayes TheoremBiopsyBloodChi-Square DistributionDiagnosis, DifferentialDNA, BacterialFemaleFeverHumansInterferon-gammaMaleMiddle AgedMycobacterium tuberculosisPleural DiseasesPleural EffusionPleural NeoplasmsPolymerase Chain ReactionPredictive Value of TestsSensitivity and SpecificitySputumTuberculosis, PleuralConceptsIFN-gamma levelsADA activity determinationPleural tuberculosisPolymerase chain reactionPleural effusionPleural fluidADA activityNonspecific clinical presentationPleural biopsy specimensTuberculous pleural effusionChain reactionAlternative diagnostic strategiesDifferent clinical settingsNegative predictive valueMeasurement of interferonAdenosine deaminase activityCombination of PCRMalignant etiologyUndetermined etiologyClinical presentationPaucibacillary natureClinical variablesPatient populationPleural biopsyDiagnostic challenge
1995
Infectivity of Trypanosoma rangeli in a promonocytic mammalian cell line.
Osorio Y, Travi B, Palma G, Saravia N. Infectivity of Trypanosoma rangeli in a promonocytic mammalian cell line. Journal Of Parasitology 1995, 81: 687-93. PMID: 7472856, DOI: 10.2307/3283955.Peer-Reviewed Original Research