Featured Publications
Chemical Probes Reveal an Extraseptal Mode of Cross-Linking in Staphylococcus aureus
Gautam S, Kim T, Spiegel DA. Chemical Probes Reveal an Extraseptal Mode of Cross-Linking in Staphylococcus aureus. Journal Of The American Chemical Society 2015, 137: 7441-7447. PMID: 26035224, DOI: 10.1021/jacs.5b02972.Peer-Reviewed Original ResearchConceptsCross-linking activityPenicillin-binding proteinsCell divisionPeptidoglycan synthesisCell wall biogenesisEnzyme cross-linking activityCell wall synthesisWall biogenesisModel organismsWall synthesisImportant human pathogenCell wallChemical probesEndogenous substratesHuman pathogensSpherical bacteriaPBP4Distinct modesPeripheral wallDivisionBiogenesisS. aureusOrganismsStaphylococcus aureusProteinWall teichoic acids prevent antibody binding to epitopes within the cell wall of Staphylococcus aureus
Gautam S, Kim T, Lester E, Deep D, Spiegel DA. Wall teichoic acids prevent antibody binding to epitopes within the cell wall of Staphylococcus aureus. ACS Chemical Biology 2015, 11: 25-30. PMID: 26502318, PMCID: PMC5592732, DOI: 10.1021/acschembio.5b00439.Peer-Reviewed Original Research
2020
Fluorescent stem peptide mimics: In situ probes for peptidoglycan crosslinking
Gautam S, Kim T, Howell R, Spiegel DA. Fluorescent stem peptide mimics: In situ probes for peptidoglycan crosslinking. Methods In Enzymology 2020, 638: 57-67. PMID: 32416921, DOI: 10.1016/bs.mie.2020.02.016.Peer-Reviewed Case Reports and Technical NotesConceptsBacterial cell wall synthesisSynthetic probesPeptide mimicsPeptidoglycan crosslinkingSynthesisSitu probeKey stepCrosslinkingNumerous classesMechanical strengthNew antibioticsProbeCell wall synthesisPeptidoglycan synthesisSuper-resolution microscopyLive bacteriaWall synthesisReactionCell wallDetailed protocolMicroscopyStaphylococcus aureusHuman pathogens