2008
MEDME: An experimental and analytical methodology for the estimation of DNA methylation levels based on microarray derived MeDIP-enrichment
Pelizzola M, Koga Y, Urban AE, Krauthammer M, Weissman S, Halaban R, Molinaro AM. MEDME: An experimental and analytical methodology for the estimation of DNA methylation levels based on microarray derived MeDIP-enrichment. Genome Research 2008, 18: 1652-1659. PMID: 18765822, PMCID: PMC2556264, DOI: 10.1101/gr.080721.108.Peer-Reviewed Original ResearchConceptsDNA methylation levelsDNA methylationMethylation levelsRelative DNA methylation levelsChromosome-wide levelBisulfite genomic DNA sequencingGenome-wide studiesMelanoma cell strainsDNA methylation statusGenomic DNA sequencingTranscriptional machineryNormal human melanocytesMethylated CpGsMethylated fragmentsEpigenetic modificationsMethylation estimatesHigh-throughput settingHuman diseasesHuman melanocytesMethylationMethylation statusDNA sequencingAntibody enrichmentGenomeCell strains
2004
Expression Profiling Reveals Novel Pathways in the Transformation of Melanocytes to Melanomas
Hoek K, Rimm DL, Williams KR, Zhao H, Ariyan S, Lin A, Kluger HM, Berger AJ, Cheng E, Trombetta ES, Wu T, Niinobe M, Yoshikawa K, Hannigan GE, Halaban R. Expression Profiling Reveals Novel Pathways in the Transformation of Melanocytes to Melanomas. Cancer Research 2004, 64: 5270-5282. PMID: 15289333, DOI: 10.1158/0008-5472.can-04-0731.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiomarkers, TumorCell Transformation, NeoplasticCohort StudiesDown-RegulationGene Expression ProfilingGene Expression Regulation, NeoplasticHumansLymphatic MetastasisMelanocytesMelanomaMiceNuclear ProteinsOligonucleotide Array Sequence AnalysisPrognosisSignal TransductionSkin NeoplasmsSurvival RateTranscription FactorsTransfectionTwist-Related Protein 1Ubiquitin ThiolesteraseConceptsGlobal differential gene expressionMembrane trafficking eventsNovel pathwayNormal melanocytesHelix protein TwistAdditional transcriptional regulatorsDifferential gene expressionMelanoma cellsTransformation of melanocytesCpG promoter methylationNormal human melanocytesTrafficking eventsTranscriptional regulatorsEmbryonic developmentGrowth suppressorChromosomal regionsExpression profilingGene expressionNotch pathwayOligonucleotide microarraysMelanoma tissue microarrayDifferential expressionGenesHuman melanocytesGrowth advantage
2000
The Regulation of Normal Melanocyte Proliferation
Halaban R. The Regulation of Normal Melanocyte Proliferation. Pigment Cell & Melanoma Research 2000, 13: 4-14. PMID: 10761990, DOI: 10.1034/j.1600-0749.2000.130103.x.Peer-Reviewed Original ResearchConceptsTranscription factorsIntracellular signal transduction cascadesE2F transcription factorsImmediate early response genesSignal transduction cascadeHepatocyte growth factor/scatter factorGrowth factorGrowth factor/scatter factorE2F transcriptional activityCyclin-dependent kinasesSynergistic growth factorsNormal human melanocytesPocket proteinsRetinoblastoma familyPeptide growth factorsStem cell factorTransduction cascadeNormal melanocyte proliferationEctopic expressionResponse genesTranscriptional activityFibroblast growth factorMolecular eventsHuman melanocytesIntermediate effectors
1996
Growth factors and melanomas.
Halaban R. Growth factors and melanomas. Seminars In Oncology 1996, 23: 673-81. PMID: 8970586.Peer-Reviewed Original ResearchMeSH KeywordsCarrier ProteinsCell Cycle ProteinsCell Transformation, NeoplasticCyclin-Dependent Kinase Inhibitor p16Cyclin-Dependent KinasesEndothelinsFibroblast Growth Factor 2Gene Expression Regulation, NeoplasticGrowth SubstancesHumansMelanocytesMelanomaReceptors, Growth FactorSignal TransductionConceptsCyclin-dependent kinasesFibroblast growth factorNormal human melanocytesMast/stem cell growth factorCell cycleCDK inhibitor p16INK4Human melanocytesMelanoma cellsGrowth factorHepatocyte growth factor/scatter factorStem cell growth factorGrowth factor/scatter factorSynergistic growth factorsNeuropeptide endothelin-1Cell cycle progressionPhosphorylation of retinoblastomaGrowth constraintsWild-type p53Unregulated expressionTranscriptional activityNegative regulatorCycle progressionResult of inactivationBasic fibroblast growth factorInhibitory complex
1995
Identification of p90RSK as the probable CREB-Ser133 kinase in human melanocytes.
Böhm M, Moellmann G, Cheng E, Alvarez-Franco M, Wagner S, Sassone-Corsi P, Halaban R. Identification of p90RSK as the probable CREB-Ser133 kinase in human melanocytes. Molecular Cancer Research 1995, 6: 291-302. PMID: 7540859.Peer-Reviewed Original ResearchMeSH KeywordsCell CountCell DivisionCells, CulturedCyclic AMPCyclic AMP Response Element-Binding ProteinDrug SynergismEndothelinsFibroblast Growth Factor 2Growth SubstancesHematopoietic Cell Growth FactorsHepatocyte Growth FactorHumansInfant, NewbornMelanocytesPhosphorylationProtein Serine-Threonine KinasesRecombinant Fusion ProteinsRibosomal Protein S6 KinasesSerineStem Cell FactorTime FactorsTranscription FactorsConceptsSynergistic growth factorsMast/stem cell growth factorHuman melanocytesGrowth factorHepatocyte growth factor/scatter factorActivation of p90RSKGrowth factor/scatter factorStem cell growth factorCAMP-responsive element binding proteinKID domainElement-binding proteinNormal human melanocytesTranscription factor 1Peptide growth factorsSer133 phosphorylationTranscriptional activationCell divisionPhosphorylated stateTranscription factorsInduces phosphorylationRegulatory proteinsSignal transducerFibroblast growth factorBasic fibroblast growth factorBinding protein
1993
Growth Regulation in Normal and Malignant Melanocytes
Halaban R. Growth Regulation in Normal and Malignant Melanocytes. Recent Results In Cancer Research 1993, 128: 133-150. PMID: 8356315, DOI: 10.1007/978-3-642-84881-0_10.Peer-Reviewed Original ResearchConceptsNormal human melanocytesHuman melanocytesSignal transduction pathwaysGrowth factorMalignant melanocytesGrowth factors/receptorsTransduction pathwaysGrowth regulationConstitutive activationBiochemical basisBiochemical eventsNormal melanocytesGrowth phaseRadial growth phaseMalignant stateMelanocytesMelanoma cellsNormal counterpartsPreneoplastic cellsReceptor activityInappropriate productionCellsMetastatic melanoma lesionsActivationReceptorsmet and HGF-SF in normal melanocytes and melanoma cells.
Halaban R, Rubin J, White W. met and HGF-SF in normal melanocytes and melanoma cells. 1993, 65: 329-39. PMID: 8380740.Peer-Reviewed Original ResearchConceptsHGF-SFFactor-dependent cellsMelanoma cellsNormal human melanocytesSignal transductionMouse melanocytesTumorigenic phenotypeHuman melanocytesHuman melanoma cellsNormal melanocytesAutocrine loopMelanocytesHuman melanomaMalignant conversionMelanin contentTyrosinase activityEctopic sitesCellsSynergistic factorExpressionBFGFERK2TransductionPhosphorylationProtein
1992
Met and hepatocyte growth factor/scatter factor signal transduction in normal melanocytes and melanoma cells.
Halaban R, Rubin J, Funasaka Y, Cobb M, Boulton T, Faletto D, Rosen E, Chan A, Yoko K, White W. Met and hepatocyte growth factor/scatter factor signal transduction in normal melanocytes and melanoma cells. Oncogene 1992, 7: 2195-206. PMID: 1331934.Peer-Reviewed Original ResearchConceptsHGF/SFMast cell growth factorSignal transductionBasic fibroblast growth factorProtein kinase/extracellular signal-regulated kinaseHuman melanocytesMalignant human melanocytesExtracellular signal-regulated kinaseHepatocyte growth factor/scatter factorGrowth factor/scatter factorSignal-regulated kinaseActivation of microtubuleProto-oncogene c-metTransmembrane tyrosine kinase receptorActivation of METC-MetTyrosine kinase receptorsNormal human melanocytesGrowth factorMouse melanocytesTyrosine phosphorylationBiological roleFibroblast growth factorKinase receptorsConstitutive activity
1991
Localization of Basic Fibroblast Growth Factor mRNA in Melanocytic Lesions by In Situ Hybridization
Scott G, Stoler M, Sarkar S, Halaban R. Localization of Basic Fibroblast Growth Factor mRNA in Melanocytic Lesions by In Situ Hybridization. Journal Of Investigative Dermatology 1991, 96: 318-322. PMID: 2002252, DOI: 10.1111/1523-1747.ep12465203.Peer-Reviewed Original ResearchConceptsBFGF gene transcriptsBasic fibroblast growth factorGene transcriptsCell populationsGrowth factorNormal human melanocytesDermal cell typesFibroblast growth factorHuman melanocytesCell typesParacrine growth factorCellular proliferationAberrant expressionSitu hybridizationMelanocytic lesionsMetastatic melanomaBasic FGF expressionFGF expressionGrowth independenceTranscriptsBasic fibroblast growth factor mRNADysplastic neviExpressionParacrine mechanismsMelanocytesGrowth factors regulating normal and malignant melanocytes
Halaban R. Growth factors regulating normal and malignant melanocytes. Cancer Treatment And Research 1991, 54: 19-40. PMID: 1673857, DOI: 10.1007/978-1-4615-3938-4_2.Peer-Reviewed Original ResearchConceptsEpidermal growth factorGrowth factor receptorV-erbEGF receptorFactor receptorGrowth factorStimulating factor receptorMacrophage-colony stimulating factor receptorEnvironmental growth factorsNormal human melanocytesRetroviral oncogenesCellular genesMitogenic signalsConstitutive activityHuman melanocytesNontransformed cellsRespective target cellsUnidentified ligandCell membraneGenesAberrant expressionNeoplastic transformationAutocrine loopNormal growthNormal cells
1988
Paracrine Stimulation of Melanocytes by Keratinocytes through Basic Fibroblast Growth Factora
HALABAN R, LANGDON R, BIRCHALL N, CCUONO C, BAIRD A, SCOTT G, MOELLOMANN G, MCGUIRE J. Paracrine Stimulation of Melanocytes by Keratinocytes through Basic Fibroblast Growth Factora. Annals Of The New York Academy Of Sciences 1988, 548: 180-190. PMID: 2470294, DOI: 10.1111/j.1749-6632.1988.tb18805.x.Peer-Reviewed Original ResearchConceptsBasic fibroblast growth factorBFGF gene transcriptsGrowth factorNormal human melanocytesGene transcriptsFibroblast growth factorNatural growth factorsNorthern blotHuman melanocytesMelanocyte mitogensMelanocyte growthParacrine stimulationMelanocytesHuman keratinocytesSynthetic peptidesKeratinocytesUltraviolet BTranscriptsPresence of keratinocytesMitogenBindingBlotProliferationMonophosphateBasic fibroblast growth factor from human keratinocytes is a natural mitogen for melanocytes.
Halaban R, Langdon R, Birchall N, Cuono C, Baird A, Scott G, Moellmann G, McGuire J. Basic fibroblast growth factor from human keratinocytes is a natural mitogen for melanocytes. Journal Of Cell Biology 1988, 107: 1611-1619. PMID: 2459134, PMCID: PMC2115244, DOI: 10.1083/jcb.107.4.1611.Peer-Reviewed Original ResearchConceptsBasic fibroblast growth factorFibroblast growth factorBFGF gene transcriptsHuman melanocytesGrowth factorNormal human melanocytesNatural mitogenHuman keratinocytesGene transcriptsNatural growth factorsNorthern blotMelanocyte mitogensMelanocyte growthMelanocytesPure culturePresence of serumMitogenSynthetic peptidesKeratinocytesCellsCAMPTranscriptsPresence of keratinocytesBindingBlotbFGF as an autocrine growth factor for human melanomas.
Halaban R, Kwon BS, Ghosh S, Delli Bovi P, Baird A. bFGF as an autocrine growth factor for human melanomas. Oncogene Research 1988, 3: 177-86. PMID: 3226725.Peer-Reviewed Original ResearchConceptsBasic fibroblast growth factorNormal human melanocytesBFGF gene transcriptsHuman melanocytesGrowth factorAutocrine growth factorGene transcriptsHuman metastatic melanomaMolecular mechanismsFibroblast growth factorNormal melanocytesCyclic adenosine monophosphateSerum-containing mediumAnti-bFGF antibodyMelanoma cellsAbnormal expressionAdenosine monophosphateHuman melanomaMelanocytesRegular culture mediumDibutyryl cyclic adenosine monophosphateMitogenic activityCulture mediumCellsTranscripts
1987
A melanocyte-specific complementary DNA clone whose expression is inducible by melanotropin and isobutylmethyl xanthine.
Kwon B, Halaban R, Kim G, Usack L, Pomerantz S, Haq A. A melanocyte-specific complementary DNA clone whose expression is inducible by melanotropin and isobutylmethyl xanthine. Molecular Biology & Medicine 1987, 4: 339-55. PMID: 2449595.Peer-Reviewed Original ResearchMeSH Keywords1-Methyl-3-isobutylxanthineAnimalsAntibodies, MonoclonalCatechol OxidaseDNAGene Expression RegulationGlycoproteinsHumansMelaninsMelanocytesMelanocyte-Stimulating HormonesMelanomaMelanoma, ExperimentalMiceMonophenol MonooxygenaseNeoplasm ProteinsPigmentationSpecies SpecificityTheophyllineTumor Cells, CulturedConceptsPmel 17CDNA clonesLambda gt11 cDNA libraryComplementary DNA cloneHuman tyrosinase geneNormal human melanocytesSingle geneDNA clonesCDNA libraryStimulation of humanMRNA speciesTyrosinase geneMurine melanocytesMurine DNAMurine melanoma cellsRestriction fragmentsHuman melanocytesRepresentative clonesGenesClonesImmunological homologyCDNAMelanoma cellsMelanocytesSpeciesTransplantation of Human Melanocytes
Lerner A, Halaban R, Klaus S, Moellmann G. Transplantation of Human Melanocytes. Journal Of Investigative Dermatology 1987, 89: 219-224. PMID: 3624895, DOI: 10.1111/1523-1747.ep12470973.Peer-Reviewed Original Research
1986
Human Melanocytes Cultured from Nevi and Melanomas
Halaban R, Ghosh S, Duray P, Kirkwood J, Lerner A. Human Melanocytes Cultured from Nevi and Melanomas. Journal Of Investigative Dermatology 1986, 87: 95-101. PMID: 2425008, DOI: 10.1111/1523-1747.ep12523594.Peer-Reviewed Original ResearchConceptsPresence of mitogensHuman melanocytesNeonatal melanocytesRate of proliferationPrimary melanomaCongenital neviMalignant transformationTransformation of melanocytesCholera toxinIsobutylmethyl xanthineHuman placentaGrowth factorProliferative rateMelanomaNewborn foreskinUnidentified factorsCell linesMelanocytesMitogenNeviHuman neonatal melanocytesPopulation doublingsMonthsLate eventProliferation
1983
Regulation of tyrosinase in human melanocytes grown in culture.
Halaban R, Pomerantz S, Marshall S, Lambert D, Lerner A. Regulation of tyrosinase in human melanocytes grown in culture. Journal Of Cell Biology 1983, 97: 480-488. PMID: 6411733, PMCID: PMC2112541, DOI: 10.1083/jcb.97.2.480.Peer-Reviewed Original ResearchConceptsHuman melanocytesApparent molecular weightMalignant human melanocytesTyrosinase activityMajor biochemical eventsExpression of tyrosinaseSynthesis of melaninRegulation of tyrosinasePosttranslational modificationsImmunoprecipitation experimentsO-glycosylationBiochemical eventsNormal melanocytesMelanocytesMolecular weightProteinEnzymeTyrosinaseTunicamycinAbundanceActivityRegulationHigh levelsLow levelsCulture