Production of RNA by a polymerase protein encapsulated within phospholipid vesicles
Chakrabarti A, Breaker R, Joyce G, Deamer D. Production of RNA by a polymerase protein encapsulated within phospholipid vesicles. Journal Of Molecular Evolution 1994, 39: 555-559. PMID: 7528810, DOI: 10.1007/bf00160400.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine DiphosphateDimyristoylphosphatidylcholineDNA-Directed RNA PolymerasesElectrophoresis, Polyacrylamide GelFluorescenceLiposomesRNAConceptsRNA polymeraseTemplate-independent RNA polymeraseProduction of RNACatalyzed polymerization reactionsBoundary membraneMacromolecular catalystsRNA productsPolymerization reactionPolymerase proteinDimyristoyl phosphatidylcholine vesiclesPolyacrylamide gel electrophoresisEthidium bromide fluorescenceEarly cellsRNA polymersPhase transition temperatureSubstrate fluxPhospholipid vesiclesVesiclesFree enzymeSuch reactionsGel electrophoresisPhosphatidylcholine vesiclesComponent lipidsPolymeraseCatalyst