2020
Unstimulated, Serum-free Cultures of Retinal Pigment Epithelium Excrete Large Mounds of Drusen-like Deposits
Chen X, Singh D, Adelman RA, Rizzolo LJ. Unstimulated, Serum-free Cultures of Retinal Pigment Epithelium Excrete Large Mounds of Drusen-like Deposits. Current Eye Research 2020, 45: 1390-1394. PMID: 32202447, DOI: 10.1080/02713683.2020.1740744.Peer-Reviewed Original ResearchMeSH KeywordsActinsApolipoproteins ECalciumCell Culture TechniquesCell LineCell ProliferationCoculture TechniquesCulture Media, Serum-FreeElectric ImpedanceHumansInduced Pluripotent Stem CellsLipid MetabolismRetinal DrusenRetinal Pigment EpitheliumStem CellsTight JunctionsTissue Inhibitor of Metalloproteinase-3VitronectinConceptsRetinal pigment epithelium
2013
Engineering a Blood-Retinal Barrier With Human Embryonic Stem Cell-Derived Retinal Pigment Epithelium: Transcriptome and Functional Analysis
Peng S, Gan G, Qiu C, Zhong M, An H, Adelman RA, Rizzolo LJ. Engineering a Blood-Retinal Barrier With Human Embryonic Stem Cell-Derived Retinal Pigment Epithelium: Transcriptome and Functional Analysis. Stem Cells Translational Medicine 2013, 2: 534-544. PMID: 23734062, PMCID: PMC3697821, DOI: 10.5966/sctm.2012-0134.Peer-Reviewed Original ResearchConceptsRetinal pigment epitheliumBlood-retinal barrierHuman retinal pigment epitheliumPigment epitheliumHuman fetal retinal pigment epitheliumOuter blood-retinal barrierTight junctionsFetal retinal pigment epitheliumEmbryonic stem cell-derived retinal pigment epitheliumAdult retinal pigment epitheliumQuantitative reverse transcription polymerase chain reactionMaturation of hESCReverse transcription-polymerase chain reactionTranscription-polymerase chain reactionHuman embryonic stem cell-derived retinal pigment epitheliumStem cell-derived retinal pigment epitheliumRPE replacement therapyPanel of genesReplacement therapyAnimal modelsHuman embryonic stem cellsRetinal degenerationRPE functionSerum-free mediumHuman retina