2017
A biodegradable scaffold enhances differentiation of embryonic stem cells into a thick sheet of retinal cells
Singh D, Wang SB, Xia T, Tainsh L, Ghiassi-Nejad M, Xu T, Peng S, Adelman RA, Rizzolo LJ. A biodegradable scaffold enhances differentiation of embryonic stem cells into a thick sheet of retinal cells. Biomaterials 2017, 154: 158-168. PMID: 29128844, DOI: 10.1016/j.biomaterials.2017.10.052.Peer-Reviewed Original ResearchConceptsRetinal degenerationNeurosensory retinaRetinal cellsHost retinal pigment epitheliumStem cellsOuter nuclear layerRetinal pigment epitheliumRetinal cell typesElaborate arborsQuantitative RT-PCRLeading causeImmune responseNuclear layerMouse modelPigment epitheliumSubretinal spaceForebrain cellsMinimal immune responseTherapeutic agentsRetinal laminationRT-PCRDegenerationRetinaRetinal organoidsConfocal immunocytochemistry
2013
Engineering a Blood-Retinal Barrier With Human Embryonic Stem Cell-Derived Retinal Pigment Epithelium: Transcriptome and Functional Analysis
Peng S, Gan G, Qiu C, Zhong M, An H, Adelman RA, Rizzolo LJ. Engineering a Blood-Retinal Barrier With Human Embryonic Stem Cell-Derived Retinal Pigment Epithelium: Transcriptome and Functional Analysis. Stem Cells Translational Medicine 2013, 2: 534-544. PMID: 23734062, PMCID: PMC3697821, DOI: 10.5966/sctm.2012-0134.Peer-Reviewed Original ResearchConceptsRetinal pigment epitheliumBlood-retinal barrierHuman retinal pigment epitheliumPigment epitheliumHuman fetal retinal pigment epitheliumOuter blood-retinal barrierTight junctionsFetal retinal pigment epitheliumEmbryonic stem cell-derived retinal pigment epitheliumAdult retinal pigment epitheliumQuantitative reverse transcription polymerase chain reactionMaturation of hESCReverse transcription-polymerase chain reactionTranscription-polymerase chain reactionHuman embryonic stem cell-derived retinal pigment epitheliumStem cell-derived retinal pigment epitheliumRPE replacement therapyPanel of genesReplacement therapyAnimal modelsHuman embryonic stem cellsRetinal degenerationRPE functionSerum-free mediumHuman retina