1995
Expression of NHE-3 in the apical membrane of rat renal proximal tubule and thick ascending limb
Amemiya M, Loffing J, Lötscher M, Kaissling B, Alpern R, Moe O. Expression of NHE-3 in the apical membrane of rat renal proximal tubule and thick ascending limb. Kidney International 1995, 48: 1206-1215. PMID: 8569082, DOI: 10.1038/ki.1995.404.Peer-Reviewed Original ResearchConceptsThick ascending limbAscending limbNHE-3H secretionNa/H exchanger isoformsProximal tubulesCortical thick ascending limbNa/H exchange activityS3 proximal tubuleDistal convoluted tubuleRat renal proximal tubulesNa/H exchangeH exchange activityRenal proximal tubulesRat NHE-3Apical membraneAbsent stainingMock-transfected cellsCortical membranesConvoluted tubulesInner stripeStrong stainingWeak stainingOuter medullaNa absorption
1991
Inhibition of Na(+)-independent H+ pump by Na(+)-induced changes in cell Ca2+.
Hays SR, Alpern RJ. Inhibition of Na(+)-independent H+ pump by Na(+)-induced changes in cell Ca2+. The Journal Of General Physiology 1991, 98: 791-813. PMID: 1660062, PMCID: PMC2229082, DOI: 10.1085/jgp.98.4.791.Peer-Reviewed Original ResearchConceptsCell pH recoveryCell Ca2PH recoveryAcid loadPump activityRenal outer medullaryDelayed activationCell acidificationRapid cell acidificationUnits/minApical membraneCell pHSustained elevationFura-2Outer medullaryExtracellular Ca2Inner stripeIntracellular Ca2Steady-state cell pHSustained increaseDimethyl-BAPTAInitial suppressionSpike increasePhysiologic ratesMicrofilament-disrupting agent
1990
Apical and basolateral membrane H+ extrusion mechanisms in inner stripe of rabbit outer medullary collecting duct
Hays SR, Alpern RJ. Apical and basolateral membrane H+ extrusion mechanisms in inner stripe of rabbit outer medullary collecting duct. American Journal Of Physiology 1990, 259: f628-f635. PMID: 2171359, DOI: 10.1152/ajprenal.1990.259.4.f628.Peer-Reviewed Original ResearchConceptsUnits/minPHi recoveryInner stripeAcid loadInitial control levelsSCH 28080Luminal Sch-28080ATPase inhibitorBasolateral membraneTriphasic responseExtrusion mechanismSlow alkalinizationRate of alkalinizationControl levelsCell acidificationRabbitsInhibitorsDuctTotal absenceInitial rateAlkalinizationN-ethylmaleimideRecoveryMinBasolateral membrane Na(+)-independent Cl-/HCO3- exchange in the inner stripe of the rabbit outer medullary collecting tubule.
Hays SR, Alpern RJ. Basolateral membrane Na(+)-independent Cl-/HCO3- exchange in the inner stripe of the rabbit outer medullary collecting tubule. The Journal Of General Physiology 1990, 95: 347-367. PMID: 2307960, PMCID: PMC2216319, DOI: 10.1085/jgp.95.2.347.Peer-Reviewed Original ResearchConceptsInner stripePeritubular ClDistal nephron segmentsBasolateral membraneCell acidificationCell pHLuminal changesUrinary acidificationNephron segmentsPH-sensitive fluorescent dyeIndependent ClCell alkalinizationCO2/HCO3Peritubular pHVoltage clampTubulesTwo-thirdsRabbitsTotal replacementCell pH changes