2016
Characterization of Cardiac Glycoside Natural Products as Potent Inhibitors of DNA Double-Strand Break Repair by a Whole-Cell Double Immunofluorescence Assay
Surovtseva YV, Jairam V, Salem AF, Sundaram RK, Bindra RS, Herzon SB. Characterization of Cardiac Glycoside Natural Products as Potent Inhibitors of DNA Double-Strand Break Repair by a Whole-Cell Double Immunofluorescence Assay. Journal Of The American Chemical Society 2016, 138: 3844-3855. PMID: 26927829, PMCID: PMC5530877, DOI: 10.1021/jacs.6b00162.Peer-Reviewed Original ResearchMeSH KeywordsCardiac GlycosidesCell Line, TumorDNA Breaks, Double-StrandedDNA End-Joining RepairFluorescent Antibody TechniqueHumansSmall Molecule LibrariesConceptsRing finger protein 8DNA double-strand break repairDouble-strand break repairSmall molecule inhibitorsDSB repairBreak repairDNA damage checkpoint protein 1DNA damage response networkNatural productsSmall moleculesP53-binding protein 1Sites of DSBsFinger protein 8Protein 1Glycoside natural productsDNA repair pathwaysNew small molecule inhibitorsClass of compoundsBinding protein 1Small molecule DNALarge compound librariesPotent inhibitorRepair pathwaysCardiac glycosidesSecondary assays
2015
Identification of Novel Radiosensitizers in a High-Throughput, Cell-Based Screen for DSB Repair Inhibitors
Goglia AG, Delsite R, Luz AN, Shahbazian D, Salem AF, Sundaram RK, Chiaravalli J, Hendrikx PJ, Wilshire JA, Jasin M, Kluger HM, Glickman JF, Powell SN, Bindra RS. Identification of Novel Radiosensitizers in a High-Throughput, Cell-Based Screen for DSB Repair Inhibitors. Molecular Cancer Therapeutics 2015, 14: 326-342. PMID: 25512618, PMCID: PMC4326563, DOI: 10.1158/1535-7163.mct-14-0765.Peer-Reviewed Original ResearchConceptsDSB repair inhibitorsDouble-strand breaksDSB repairHomologous recombinationRepair inhibitorsCell-based small molecule screenSuccessful DSB repairDNA-damaging agentsPlate-based formatCell-based screenSmall-molecule screenGenomic integrityTumor cell survivalMammalian cellsHR repairDNA repairMolecule screenReporter systemSecondary assaysCell survivalDNA damageCancer cell linesTumor cellsNovel hitsMost cancer therapies