2024
Overlapping role of synaptophysin and synaptogyrin family proteins in determining the small size of synaptic vesicles
Park D, Fujise K, Wu Y, Luján R, Del Olmo-Cabrera S, Wesseling J, De Camilli P. Overlapping role of synaptophysin and synaptogyrin family proteins in determining the small size of synaptic vesicles. Proceedings Of The National Academy Of Sciences Of The United States Of America 2024, 121: e2409605121. PMID: 38985768, PMCID: PMC11260120, DOI: 10.1073/pnas.2409605121.Peer-Reviewed Original ResearchConceptsSynaptic vesiclesFamily proteinsBiogenesis of synaptic vesiclesClusters of small vesiclesSize of synaptic vesiclesSynaptogyrin familySynaptogyrin-1Vesicle proteinsSynaptogyrinTransmembrane domainOrganismal levelSmall vesiclesProteinMild defectsVesiclesFamily membersBiogenesisSmall sizeFamilyMiceSynapsinCoexpressionAbundanceSynaptoporinMembers
2023
Synaptic vesicle proteins and ATG9A self-organize in distinct vesicle phases within synapsin condensates
Park D, Wu Y, Wang X, Gowrishankar S, Baublis A, De Camilli P. Synaptic vesicle proteins and ATG9A self-organize in distinct vesicle phases within synapsin condensates. Nature Communications 2023, 14: 455. PMID: 36709207, PMCID: PMC9884207, DOI: 10.1038/s41467-023-36081-3.Peer-Reviewed Original ResearchConceptsMembrane proteinsSV membrane proteinsVesicle membrane proteinEctopic expression systemSynaptic vesicle proteinsSynaptic vesicle clustersSV proteinsVesicle proteinsEctopic expressionExpression systemVesicle clustersSynapsinProteinLiquid-like propertiesVesiclesDistinct classesDual roleSynapsin 1FibroblastsATG9ASynapsesExpressionDistinct phasesNerve terminalsClusters
2021
Cooperative function of synaptophysin and synapsin in the generation of synaptic vesicle-like clusters in non-neuronal cells
Park D, Wu Y, Lee SE, Kim G, Jeong S, Milovanovic D, De Camilli P, Chang S. Cooperative function of synaptophysin and synapsin in the generation of synaptic vesicle-like clusters in non-neuronal cells. Nature Communications 2021, 12: 263. PMID: 33431828, PMCID: PMC7801664, DOI: 10.1038/s41467-020-20462-z.Peer-Reviewed Original ResearchConceptsNon-neuronal cellsSV clustersSynaptic vesiclesSmall synaptic-like microvesiclesSV membrane proteinsSynaptic-like microvesiclesSV proteinsDiffuse cytosolic distributionMembrane proteinsReconstitution systemCytosolic distributionCooperative functionSuch vesiclesMechanistic insightsLiquid-like propertiesPowerful modelPhysiological formationProteinSynapsinVesiclesCellsSynaptic transmissionAssembly of structuresDefining featureLiquid condensate
2018
A liquid phase of synapsin and lipid vesicles
Milovanovic D, Wu Y, Bian X, De Camilli P. A liquid phase of synapsin and lipid vesicles. Science 2018, 361: 604-607. PMID: 29976799, PMCID: PMC6191856, DOI: 10.1126/science.aat5671.Peer-Reviewed Original ResearchConceptsSynaptic vesiclesNeurotransmitter-containing synaptic vesiclesCalmodulin-dependent protein kinase IILipid vesiclesCalcium/calmodulin-dependent protein kinase IIProtein kinase IISmall lipid vesiclesKinase IILiquid-liquid phase separationSynapsinDistinct liquid phasesVesiclesTight clusterSynapsin 1Presynaptic sitesPhosphorylationExocytosis
1990
Synapsins in the vertebrate retina: Absence from ribbon synapses and heterogeneous distribution among conventional synapses
Mandell J, Townes-Anderson E, Czernik A, Cameron R, Greengard P, De Camilli P. Synapsins in the vertebrate retina: Absence from ribbon synapses and heterogeneous distribution among conventional synapses. Neuron 1990, 5: 19-33. PMID: 2114884, DOI: 10.1016/0896-6273(90)90030-j.Peer-Reviewed Original ResearchConceptsConventional synapsesRibbon synapsesAmacrine cellsBipolar cellsIntrinsic membrane proteinsSynapsin IAdult rat retinaNerve terminal phosphoproteinVertebrate retinaMembrane proteinsNerve terminalsRat retinaVesicle clusteringSalamander retinaSynapse typesSynaptic vesiclesMolecular differencesRetinaRod cellsSynapsesCone cellsSynapsinCellsSynaptophysinPhosphoprotein
1989
Synapsins: Mosaics of Shared and Individual Domains in a Family of Synaptic Vesicle Phosphoproteins
Südhof T, Czernik A, Kao H, Takei K, Johnston P, Horiuchi A, Kanazir S, Wagner M, Perin M, De Camilli P, Greengard P. Synapsins: Mosaics of Shared and Individual Domains in a Family of Synaptic Vesicle Phosphoproteins. Science 1989, 245: 1474-1480. PMID: 2506642, DOI: 10.1126/science.2506642.Peer-Reviewed Original ResearchConceptsDistinct signal transduction pathwaysHomologous amino-terminal domainsAmino-terminal domainCarboxyl-terminal domainSignal transduction pathwaysNeurotransmitter releaseDifferential splicingMolecular cloningTransduction pathwaysSynaptic vesicle phosphoproteinsHomologous proteinsNeuronal phosphoproteinSynaptic vesiclesMessenger RNASynapsinStructural diversityIndividual domainsPhosphoproteinDifferential distributionTypes of neuronsRelative amountsDomainSplicingCytoskeletonDifferent roles