2020
Enteric Oxalate Secretion Mediated by Slc26a6 Defends against Hyperoxalemia in Murine Models of Chronic Kidney Disease
Neumeier LI, Thomson RB, Reichel M, Eckardt KU, Aronson PS, Knauf F. Enteric Oxalate Secretion Mediated by Slc26a6 Defends against Hyperoxalemia in Murine Models of Chronic Kidney Disease. Journal Of The American Society Of Nephrology 2020, 31: 1987-1995. PMID: 32660969, PMCID: PMC7461683, DOI: 10.1681/asn.2020010105.Peer-Reviewed Original ResearchConceptsEnteric oxalate secretionPlasma oxalate concentrationOxalate secretionModel of CKDChronic kidney diseaseIntestine of miceWild-type miceHealthy kidney functionOxalate clearanceWestern blot analysisKidney injuryKidney functionOxalate excretionWeekly injectionsKidney diseaseCKD modelExtrarenal clearanceOxalate transporter SLC26A6CKDMurine modelSignificant elevationOxalate homeostasisTransporter expressionMiceProtein expression
2016
Loss of Cystic Fibrosis Transmembrane Regulator Impairs Intestinal Oxalate Secretion
Knauf F, Thomson RB, Heneghan JF, Jiang Z, Adebamiro A, Thomson CL, Barone C, Asplin JR, Egan ME, Alper SL, Aronson PS. Loss of Cystic Fibrosis Transmembrane Regulator Impairs Intestinal Oxalate Secretion. Journal Of The American Society Of Nephrology 2016, 28: 242-249. PMID: 27313231, PMCID: PMC5198290, DOI: 10.1681/asn.2016030279.Peer-Reviewed Original ResearchConceptsIntestinal oxalate secretionWild-type miceCystic fibrosisIntestinal tissueOxalate secretionIncidence of hyperoxaluriaCalcium oxalate stone formationNet intestinal absorptionOxalate stone formationCoexpression of CFTRIntestinal transport processesWestern blot analysisOxalate absorptionMouse modelIntestinal absorptionGlucose absorptionUssing chambersStone formationFibrosisMiceSecretionReduced expressionCystic fibrosis transmembrane conductance regulator (CFTR) geneHyperoxaluriaPatients
2014
Cyclic GMP Kinase II (cGKII) Inhibits NHE3 by Altering Its Trafficking and Phosphorylating NHE3 at Three Required Sites IDENTIFICATION OF A MULTIFUNCTIONAL PHOSPHORYLATION SITE*
Chen T, Kocinsky HS, Cha B, Murtazina R, Yang J, Tse CM, Singh V, Cole R, Aronson PS, de Jonge H, Sarker R, Donowitz M. Cyclic GMP Kinase II (cGKII) Inhibits NHE3 by Altering Its Trafficking and Phosphorylating NHE3 at Three Required Sites IDENTIFICATION OF A MULTIFUNCTIONAL PHOSPHORYLATION SITE*. Journal Of Biological Chemistry 2014, 290: 1952-1965. PMID: 25480791, PMCID: PMC4303652, DOI: 10.1074/jbc.m114.590174.Peer-Reviewed Original ResearchAnimalsBinding SitesCaco-2 CellsCell MembraneCyclic GMP-Dependent Protein Kinase Type IIDexamethasoneHumansIntestinal MucosaMass SpectrometryMiceMicrovilliMutagenesisPhosphorylationProtein Structure, TertiaryProtein TransportSerineSodium-Hydrogen Exchanger 3Sodium-Hydrogen ExchangersSurface PropertiesTransfection
2011
Net Intestinal Transport of Oxalate Reflects Passive Absorption and SLC26A6-mediated Secretion
Knauf F, Ko N, Jiang Z, Robertson WG, Van Itallie CM, Anderson JM, Aronson PS. Net Intestinal Transport of Oxalate Reflects Passive Absorption and SLC26A6-mediated Secretion. Journal Of The American Society Of Nephrology 2011, 22: 2247-2255. PMID: 22021714, PMCID: PMC3250206, DOI: 10.1681/asn.2011040433.Peer-Reviewed Original ResearchCholinergic signaling inhibits oxalate transport by human intestinal T84 cells
Hassan HA, Cheng M, Aronson PS. Cholinergic signaling inhibits oxalate transport by human intestinal T84 cells. American Journal Of Physiology - Cell Physiology 2011, 302: c46-c58. PMID: 21956166, PMCID: PMC3328906, DOI: 10.1152/ajpcell.00075.2011.Peer-Reviewed Original ResearchConceptsC-SrcT84 cellsHuman intestinal cell line T84PKC-δ inhibitor rottlerinSrc inhibitor PP2Intestinal cell line T84Human intestinal T84 cellsSurface expressionMuscarinic receptorsRegulation downstreamInhibitor PP2Phosphorylation studiesBiotinylation studiesInhibitor rottlerinKnockdown studiesPharmacological inhibitorsIntestinal T84 cellsIntestinal oxalate secretionPhospholipase CIntestinal ion transportPKC activationSignificant translocationCalcium oxalate urolithiasisPKCAnion exchanger SLC26A6
1990
Characterization of Na(+)-H+ exchangers on villus cells in rabbit ileum
Knickelbein RG, Aronson PS, Dobbins JW. Characterization of Na(+)-H+ exchangers on villus cells in rabbit ileum. American Journal Of Physiology 1990, 259: g802-g806. PMID: 2173416, DOI: 10.1152/ajpgi.1990.259.5.g802.Peer-Reviewed Original ResearchRabbit ileal brush-border membrane Cl-HCO3 exchanger is activated by an internal pH-sensitive modifier site
Mugharbil A, Knickelbein RG, Aronson PS, Dobbins JW. Rabbit ileal brush-border membrane Cl-HCO3 exchanger is activated by an internal pH-sensitive modifier site. American Journal Of Physiology 1990, 259: g666-g670. PMID: 2171352, DOI: 10.1152/ajpgi.1990.259.4.g666.Peer-Reviewed Original Research
1986
Oxalate transport by anion exchange across rabbit ileal brush border.
Knickelbein RG, Aronson PS, Dobbins JW. Oxalate transport by anion exchange across rabbit ileal brush border. Journal Of Clinical Investigation 1986, 77: 170-175. PMID: 3003149, PMCID: PMC423323, DOI: 10.1172/jci112272.Peer-Reviewed Original ResearchConceptsInside-alkaline pH gradientOxalate uptakeBrush border membraneCl- gradientRabbit ileal brush borderBrush borderIleal brush borderBrush border componentFivefold accumulationFourfold accumulationBorder membraneAbsence of HCO3Alkaline pH gradientRabbit ileumHCO3- exchangeDensity gradient centrifugationBasolateral components
1985
Sodium and chloride transport across rabbit ileal brush border. II. Evidence for Cl-HCO3 exchange and mechanism of coupling
Knickelbein R, Aronson PS, Schron CM, Seifter J, Dobbins JW. Sodium and chloride transport across rabbit ileal brush border. II. Evidence for Cl-HCO3 exchange and mechanism of coupling. American Journal Of Physiology 1985, 249: g236-g245. PMID: 3927745, DOI: 10.1152/ajpgi.1985.249.2.g236.Peer-Reviewed Original ResearchMeSH Keywords4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic AcidAmilorideAnimalsAnionsBicarbonatesCarbonic AnhydrasesChloridesHydrogen-Ion ConcentrationHydroxidesIleumIn Vitro TechniquesIntestinal AbsorptionIntestinal MucosaMembrane PotentialsMicrovilliModels, BiologicalPotassiumRabbitsSodiumConceptsStimulation of ClBrush border membraneCl- uptakeInside-alkaline pH gradientCl transportCl-HCO3 exchangeRabbit ileumCoupling of NaX minMM ClBrush border vesiclesBrush borderN2-5Saturable functionMM KClNa uptakeLevel fourfoldAddition of HCO3K diffusion potentialsCarbonic anhydrase activityChloride transportUptakeSubstrate and inhibitor specificity of anion exchangers on the brush border membrane of rabbit ileum
Knickelbein R, Aronson P, Dobbins J. Substrate and inhibitor specificity of anion exchangers on the brush border membrane of rabbit ileum. The Journal Of Membrane Biology 1985, 88: 199-204. PMID: 3005584, DOI: 10.1007/bf01868433.Peer-Reviewed Original Research
1983
Sodium and chloride transport across rabbit ileal brush border. I. Evidence for Na-H exchange
Knickelbein R, Aronson PS, Atherton W, Dobbins JW. Sodium and chloride transport across rabbit ileal brush border. I. Evidence for Na-H exchange. American Journal Of Physiology 1983, 245: g504-g510. PMID: 6624918, DOI: 10.1152/ajpgi.1983.245.4.g504.Peer-Reviewed Original Research
1980
Mechanism of increased potassium secretion in potassium loading and sodium deprivation
Hayslett J, Myketey N, Binder H, Aronson P. Mechanism of increased potassium secretion in potassium loading and sodium deprivation. American Journal Of Physiology 1980, 239: f378-f382. PMID: 6252786, DOI: 10.1152/ajprenal.1980.239.4.f378.Peer-Reviewed Original ResearchConceptsPlasma membrane-rich fractionMembrane-rich fractionBasolateral cell membraneCell membraneBasolateral membraneColonic cellsEnzyme activityK adaptationNa-K-ATPase activityMembraneColonic mucosal cellsEnzymeCellsMarker enzymesSpecific bindingOuabain-binding sitesAdenylate cyclaseSodium-deprived animalsAnimalsProteinK pumpActivityMucosal cellsATPSites