2001
Association of Na+-H+ Exchanger Isoform NHE3 and Dipeptidyl Peptidase IV in the Renal Proximal Tubule*
Girardi A, Degray B, Nagy T, Biemesderfer D, Aronson P. Association of Na+-H+ Exchanger Isoform NHE3 and Dipeptidyl Peptidase IV in the Renal Proximal Tubule*. Journal Of Biological Chemistry 2001, 276: 46671-46677. PMID: 11590171, DOI: 10.1074/jbc.m106897200.Peer-Reviewed Original ResearchConceptsApical membraneExchanger Isoform NHE3Dipeptidyl peptidase IVN-terminal sequencingProtein complexesDPPIV enzyme activityRenal microvillus membrane vesiclesMicrovillar fractionMembrane populationsMembrane vesiclesPeptidase IVWestern blot analysisMicrovillus membrane vesiclesNHE3 surface expressionBrush borderImmunoelectron microscopyRabbit renal brush borderBlot analysisProteinEnzyme activitySurface expressionMicrovillus membraneNHE3Different poolsImmunoaffinity chromatography
1998
Immunochemical characterization of Na+/H+exchanger isoform NHE4
Pizzonia J, Biemesderfer D, Abu-Alfa A, Wu M, Exner M, Isenring P, Igarashi P, Aronson P. Immunochemical characterization of Na+/H+exchanger isoform NHE4. American Journal Of Physiology 1998, 275: f510-f517. PMID: 9755122, DOI: 10.1152/ajprenal.1998.275.4.f510.Peer-Reviewed Original ResearchConceptsSubcellular localizationFusion proteinWestern blot analysisBlot analysisBroad tissue distributionBasolateral membraneTransport proteinsMembrane fractionProteinEpithelial cellsSkeletal muscleGradient centrifugationTissue distributionPercoll gradient centrifugationCellsGastric glandsNHE4MembraneImmunochemical characterizationMammalianLocalizationRat tissuesImmunochemical techniquesMonoclonal antibodiesPolypeptide
1995
Isolation and cDNA Cloning of Ksp-cadherin, a Novel Kidney-specific Member of the Cadherin Multigene Family *
Thomson R, Igarashi P, Biemesderfer D, Kim R, Abu-Alfa A, Soleimani M, Aronson P. Isolation and cDNA Cloning of Ksp-cadherin, a Novel Kidney-specific Member of the Cadherin Multigene Family *. Journal Of Biological Chemistry 1995, 270: 17594-17601. PMID: 7615566, DOI: 10.1074/jbc.270.29.17594.Peer-Reviewed Original ResearchConceptsKidney-specific memberMultigene familyKsp-cadherinCDNA cloningCharacteristic cysteine residuesT7 expression systemClassical cadherinsCytoplasmic domainCysteine residuesExpression systemTissue developmentImmunolocalization studiesRecognition sequenceNorthern analysisCadherinDemonstrated roleCellular recognitionMolecular analysisLI-cadherinHPT-1Basolateral membraneCloningRenal tubular epithelial cellsEpithelial cellsProtein
1993
NHE3: a Na+/H+ exchanger isoform of renal brush border
Biemesderfer D, Pizzonia J, Abu-Alfa A, Exner M, Reilly R, Igarashi P, Aronson PS. NHE3: a Na+/H+ exchanger isoform of renal brush border. American Journal Of Physiology 1993, 265: f736-f742. PMID: 8238556, DOI: 10.1152/ajprenal.1993.265.5.f736.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceDNA PrimersFluorescent Antibody TechniqueImmunoblottingImmunohistochemistryKidneyKidney CortexKidney Tubules, ProximalL CellsMiceMicrovilliMolecular Sequence DataMolecular WeightPolymerase Chain ReactionRabbitsRecombinant ProteinsSodium-Hydrogen ExchangersTransfectionConceptsBrush borderRabbit kidneyBrush border membraneProximal tubule cellsRenal brush borderRenal proximal tubulesWestern blot analysisImmune serumProximal tubulesTubule cellsGuinea pigsImmunocytochemical studyRenal brush border membraneKidneyTranscellular reabsorptionExchanger isoformsNHE3Blot analysisFusion proteinSpecific reactivityBasolateral membraneExchanger geneCellsProtein
1992
cDNA cloning and localization of a band 3-related protein from ileum
Chow A, Dobbins JW, Aronson PS, Igarashi P. cDNA cloning and localization of a band 3-related protein from ileum. American Journal Of Physiology 1992, 263: g345-g352. PMID: 1415547, DOI: 10.1152/ajpgi.1992.263.3.g345.Peer-Reviewed Original ResearchConceptsRabbit ileal enterocytesDeduced amino acid sequenceBand 3Anion exchanger familyAmino acid sequenceAE gene familyBrush border membraneGene familyNonerythroid tissuesHCO3- exchangerErythroid band 3CDNA cloningPlasma membraneAcid sequenceExchanger familyRelated proteinsMembrane vesiclesIleal enterocytesMolecular identityCytoplasmic fragmentsProteinBasolateral membrane vesiclesBasolateral membraneMembraneEnterocytes
1991
cDNA cloning and immunolocalization of a Na(+)-H+ exchanger in LLC-PK1 renal epithelial cells
Reilly RF, Hildebrandt F, Biemesderfer D, Sardet C, Pouyssegur J, Aronson PS, Slayman CW, Igarashi P. cDNA cloning and immunolocalization of a Na(+)-H+ exchanger in LLC-PK1 renal epithelial cells. American Journal Of Physiology 1991, 261: f1088-f1094. PMID: 1661081, DOI: 10.1152/ajprenal.1991.261.6.f1088.Peer-Reviewed Original ResearchConceptsLLC-PK1 cellsFusion proteinAmino-terminal hydrophobic regionLLC-PK1 renal epithelial cellsHydrophobic regionAmino acid sequenceConfluent LLC-PK1 cellsRelative molecular massRenal epithelial cellsSignificant homologyHuman proteinsCDNA cloningAcid sequenceNucleotide identityHuman sequenceLibrary screeningCDNAMolecular massPolymerase chain reactionAmino acidsApical membraneProteinConfocal microscopyHydrophilic domainsLLC-PK1
1980
Mechanism of increased potassium secretion in potassium loading and sodium deprivation
Hayslett J, Myketey N, Binder H, Aronson P. Mechanism of increased potassium secretion in potassium loading and sodium deprivation. American Journal Of Physiology 1980, 239: f378-f382. PMID: 6252786, DOI: 10.1152/ajprenal.1980.239.4.f378.Peer-Reviewed Original ResearchConceptsPlasma membrane-rich fractionMembrane-rich fractionBasolateral cell membraneCell membraneBasolateral membraneColonic cellsEnzyme activityK adaptationNa-K-ATPase activityMembraneColonic mucosal cellsEnzymeCellsMarker enzymesSpecific bindingOuabain-binding sitesAdenylate cyclaseSodium-deprived animalsAnimalsProteinK pumpActivityMucosal cellsATPSites