2006
Role of SLC26‐Mediated Cl−/Base Exchange in Proximal Tubule NaCl Transport
Aronson PS. Role of SLC26‐Mediated Cl−/Base Exchange in Proximal Tubule NaCl Transport. Novartis Foundation Symposia 2006, 273: 148-163. PMID: 17120766, DOI: 10.1002/0470029579.ch10.Commentaries, Editorials and LettersIon exchangers mediating Na+, HCO3 - and Cl- transport in the renal proximal tubule.
Aronson PS. Ion exchangers mediating Na+, HCO3 - and Cl- transport in the renal proximal tubule. Journal Of Nephrology 2006, 19 Suppl 9: s3-s10. PMID: 16736438.Commentaries, Editorials and LettersConceptsProximal tubulesNaCl absorptionApical membrane ClNull miceOxalate-sulfate exchangePendrin-null miceProximal tubule cellsBrush borderFormate exchangeRenal proximal tubulesSlc26a6-null miceAcid secretionPendrin expressionFunctional expression studiesTubule cellsNaCl reabsorptionCl absorptionBrush border membraneSulfate cotransportLines of evidenceExchanger isoformsTubulesMiceFamily membersBorder membrane
2005
Use of phospho-specific antibodies to determine the phosphorylation of endogenous Na+/H+ exchanger NHE3 at PKA consensus sites
Kocinsky HS, Girardi AC, Biemesderfer D, Nguyen T, Mentone S, Orlowski J, Aronson PS. Use of phospho-specific antibodies to determine the phosphorylation of endogenous Na+/H+ exchanger NHE3 at PKA consensus sites. American Journal Of Physiology. Renal Physiology 2005, 289: f249-f258. PMID: 15687252, DOI: 10.1152/ajprenal.00082.2004.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodiesAntibodies, MonoclonalAntibody SpecificityCell LineChlorocebus aethiopsCOS CellsCyclic AMP-Dependent Protein KinasesDopamineElectrophoresis, Polyacrylamide GelImmunohistochemistryKidneyMaleMiceMice, Inbred BALB CMicroscopy, FluorescenceMicrovilliOpossumsPhosphorylationRatsRats, Sprague-DawleySodiumSodium-Hydrogen Exchanger 3Sodium-Hydrogen ExchangersSubcellular FractionsTransfectionConceptsSerine 552Total NHE3Brush border membranePKA consensus sitesEffects of dopamineProximal tubule cellsWestern blot assaysOpossum kidney cellsBaseline phosphorylationPeptide antibodiesInhibition of NHE3Tubule cellsBlot assaysAntibodiesPhosphorylation of NHE3Phosphospecific antibodiesExchanger NHE3NHE3DopamineEndogenous NHE3Kidney cellsVivoPhospho-specific antibodiesTransfection studiesVitro
2004
Role of dipeptidyl peptidase IV in regulating activity of Na+/H+ exchanger isoform NHE3 in proximal tubule cells
Girardi AC, Knauf F, Demuth HU, Aronson PS. Role of dipeptidyl peptidase IV in regulating activity of Na+/H+ exchanger isoform NHE3 in proximal tubule cells. American Journal Of Physiology - Cell Physiology 2004, 287: c1238-c1245. PMID: 15213057, DOI: 10.1152/ajpcell.00186.2004.Peer-Reviewed Original ResearchConceptsProximal tubule cellsDPPIV inhibitorsDipeptidyl peptidase IVTubule cellsNHE3 activityOpossum kidney proximal tubule cellsKidney proximal tubule cellsNHE3 surface expressionPeptidase IVAdditive inhibitory effectEffects of genisteinForskolin/IBMXTyrosine kinase inhibitor genisteinSpecific competitive inhibitorPKA inhibitor HKinase inhibitor genisteinP32/98Activation of PKAInhibitory effectRenal brush border membraneBrush border membraneSurface expressionProtein kinase pathwayNHE3Inhibitor genistein
2002
Specificity of Anion Exchange Mediated by Mouse Slc26a6*
Jiang Z, Grichtchenko II, Boron WF, Aronson PS. Specificity of Anion Exchange Mediated by Mouse Slc26a6*. Journal Of Biological Chemistry 2002, 277: 33963-33967. PMID: 12119287, DOI: 10.1074/jbc.m202660200.Peer-Reviewed Original ResearchConceptsProximal tubule cellsP-aminohippurateTubule cellsOxalate-sulfate exchangeTransport of oxalatePH recordingRenal tubular fluidTubular fluidVoltage-clamp measurementsBrush border membraneFormate exchangeOxalate exchangeMeasurement of uptakeClamp measurementsBorder membraneSlc26a6Two-electrode voltage clamp measurementsXenopus oocytesPresent studyHigh oxalate concentrationsApical membraneOocytesMouse orthologueCellsCFEX
1997
Ion exchangers mediating NaCl transport in the proximal tubule.
Aronson PS. Ion exchangers mediating NaCl transport in the proximal tubule. Wiener Klinische Wochenschrift 1997, 109: 435-40. PMID: 9261983.Commentaries, Editorials and LettersConceptsApical membraneBasolateral membraneBrush border membraneMembrane traffickingNHE3 protein expressionMembrane ion exchangerIntracellular vesiclesMammalian proximal tubuleBorder membraneDifferential regulationProximal tubule cellsInhibitor sensitivityPossible regulationFormate transportExchange activityProtein expressionCl- channelsTubule cellsMembranePolyclonal antibodiesVesiclesCellsNHE3Oxalate-sulfate exchangeRegulationExpression of Na(+)-H+ exchanger isoforms NHE1 and NHE3 in kidney and blood cells of rabbit and rat.
Rutherford PA, Pizzonia JH, Biemesderfer D, Abu-Alfa A, Reilly R, Aronson PS. Expression of Na(+)-H+ exchanger isoforms NHE1 and NHE3 in kidney and blood cells of rabbit and rat. Nephron 1997, 5: 490-7. PMID: 9438178.Peer-Reviewed Original ResearchConceptsNa-H exchangeNa-H exchangerBlood cellsRat kidneyNa-Li countertransportNHE1 protein expressionExpression of NHE1Diabetic nephropathyCountertransport activityIsoform-specific antibodiesMonoclonal antibodiesExchanger isoforms NHE1NHE3 expressionRenal brush border membraneProtein expressionBrush border membraneKidneyNHE isoformsRabbitsNHE3Blood cell membranesBrush border membrane fractionRatsIsoforms NHE1Antibodies
1996
Role of NHE3 in Mediating Renal Brush Border Na+-H+ Exchange ADAPTATION TO METABOLIC ACIDOSIS*
Wu M, Biemesderfer D, Giebisch G, Aronson P. Role of NHE3 in Mediating Renal Brush Border Na+-H+ Exchange ADAPTATION TO METABOLIC ACIDOSIS*. Journal Of Biological Chemistry 1996, 271: 32749-32752. PMID: 8955109, DOI: 10.1074/jbc.271.51.32749.Peer-Reviewed Original ResearchConceptsMetabolic acidosisControl ratsBrush border membraneRenal brush border vesiclesNHE3 proteinBrush border vesiclesBorder membraneBrush borderNHE3 protein expressionRole of NHE3Western blot analysisNormal ratsAcidosisRatsAcidotic ratsRenal brush border membranePharmacologic inhibitorsProtein expressionExchange activityInhibitor sensitivityNHE3Renal brush borderBlot analysisRole of ion exchangers in mediating NaCl transport in the proximal tubule
Aronson P. Role of ion exchangers in mediating NaCl transport in the proximal tubule. Kidney International 1996, 49: 1665-1670. PMID: 8743473, DOI: 10.1038/ki.1996.243.Commentaries, Editorials and LettersConceptsProximal tubulesOxalate-sulfate exchangeProximal tubule cellsNaCl transportRecycling of formateTranscellular NaCl transportMetabolic acidosisApical membraneRenal maturationReabsorption of NaClTubule cellsNaCl reabsorptionImmunocytochemical studyNHE3 proteinBrush border membraneSulfate cotransport
1993
NHE3: a Na+/H+ exchanger isoform of renal brush border
Biemesderfer D, Pizzonia J, Abu-Alfa A, Exner M, Reilly R, Igarashi P, Aronson PS. NHE3: a Na+/H+ exchanger isoform of renal brush border. American Journal Of Physiology 1993, 265: f736-f742. PMID: 8238556, DOI: 10.1152/ajprenal.1993.265.5.f736.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceDNA PrimersFluorescent Antibody TechniqueImmunoblottingImmunohistochemistryKidneyKidney CortexKidney Tubules, ProximalL CellsMiceMicrovilliMolecular Sequence DataMolecular WeightPolymerase Chain ReactionRabbitsRecombinant ProteinsSodium-Hydrogen ExchangersTransfectionConceptsBrush borderRabbit kidneyBrush border membraneProximal tubule cellsRenal brush borderRenal proximal tubulesWestern blot analysisImmune serumProximal tubulesTubule cellsGuinea pigsImmunocytochemical studyRenal brush border membraneKidneyTranscellular reabsorptionExchanger isoformsNHE3Blot analysisFusion proteinSpecific reactivityBasolateral membraneExchanger geneCellsProtein
1992
cDNA cloning and localization of a band 3-related protein from ileum
Chow A, Dobbins JW, Aronson PS, Igarashi P. cDNA cloning and localization of a band 3-related protein from ileum. American Journal Of Physiology 1992, 263: g345-g352. PMID: 1415547, DOI: 10.1152/ajpgi.1992.263.3.g345.Peer-Reviewed Original ResearchConceptsRabbit ileal enterocytesDeduced amino acid sequenceBand 3Anion exchanger familyAmino acid sequenceAE gene familyBrush border membraneGene familyNonerythroid tissuesHCO3- exchangerErythroid band 3CDNA cloningPlasma membraneAcid sequenceExchanger familyRelated proteinsMembrane vesiclesIleal enterocytesMolecular identityCytoplasmic fragmentsProteinBasolateral membrane vesiclesBasolateral membraneMembraneEnterocytes
1986
Oxalate transport by anion exchange across rabbit ileal brush border.
Knickelbein RG, Aronson PS, Dobbins JW. Oxalate transport by anion exchange across rabbit ileal brush border. Journal Of Clinical Investigation 1986, 77: 170-175. PMID: 3003149, PMCID: PMC423323, DOI: 10.1172/jci112272.Peer-Reviewed Original ResearchConceptsInside-alkaline pH gradientOxalate uptakeBrush border membraneCl- gradientRabbit ileal brush borderBrush borderIleal brush borderBrush border componentFivefold accumulationFourfold accumulationBorder membraneAbsence of HCO3Alkaline pH gradientRabbit ileumHCO3- exchangeDensity gradient centrifugationBasolateral components
1985
Sodium and chloride transport across rabbit ileal brush border. II. Evidence for Cl-HCO3 exchange and mechanism of coupling
Knickelbein R, Aronson PS, Schron CM, Seifter J, Dobbins JW. Sodium and chloride transport across rabbit ileal brush border. II. Evidence for Cl-HCO3 exchange and mechanism of coupling. American Journal Of Physiology 1985, 249: g236-g245. PMID: 3927745, DOI: 10.1152/ajpgi.1985.249.2.g236.Peer-Reviewed Original ResearchMeSH Keywords4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic AcidAmilorideAnimalsAnionsBicarbonatesCarbonic AnhydrasesChloridesHydrogen-Ion ConcentrationHydroxidesIleumIn Vitro TechniquesIntestinal AbsorptionIntestinal MucosaMembrane PotentialsMicrovilliModels, BiologicalPotassiumRabbitsSodiumConceptsStimulation of ClBrush border membraneCl- uptakeInside-alkaline pH gradientCl transportCl-HCO3 exchangeRabbit ileumCoupling of NaX minMM ClBrush border vesiclesBrush borderN2-5Saturable functionMM KClNa uptakeLevel fourfoldAddition of HCO3K diffusion potentialsCarbonic anhydrase activityChloride transportUptake