2024
UBR5 promotes antiviral immunity by disengaging the transcriptional brake on RIG-I like receptors
Yang D, Geng T, Harrison A, Cahoon J, Xing J, Jiao B, Wang M, Cheng C, Hill R, Wang H, Vella A, Cheng G, Wang Y, Wang P. UBR5 promotes antiviral immunity by disengaging the transcriptional brake on RIG-I like receptors. Nature Communications 2024, 15: 780. PMID: 38278841, PMCID: PMC10817939, DOI: 10.1038/s41467-024-45141-1.Peer-Reviewed Original ResearchConceptsUbiquitin protein ligase E3 component N-recognin 5RIG-IImmune response to RNA virusesResponse to RNA virusesRetinoic acid-inducible gene ILysine 63-linked ubiquitinationAntiviral immune responseRNA virus infectionViral RNA sensorsKnockout cell linesInitiation of antiviral immune responsesBoost antiviral immune responseImmune responsePosttranslational regulationTranscriptional brakeGene IEpigenetic repressorsRNA virusesDe-SUMOylationRNA sensorsUbiquitinWild type littermatesIncreased viral replicationTRIM28Transcription
2018
Foot-and-mouth disease virus capsid protein VP2 activates the cellular EIF2S1-ATF4 pathway and induces autophagy via HSPB1
Sun P, Zhang S, Qin X, Chang X, Cui X, Li H, Zhang S, Gao H, Wang P, Zhang Z, Luo J, Li Z. Foot-and-mouth disease virus capsid protein VP2 activates the cellular EIF2S1-ATF4 pathway and induces autophagy via HSPB1. Autophagy 2018, 14: 336-346. PMID: 29166823, PMCID: PMC5902195, DOI: 10.1080/15548627.2017.1405187.Peer-Reviewed Original ResearchConceptsCapsid protein VP2FMDV infectionProtein VP2Polyglutamine expansion proteinsMacroautophagy/autophagyMammalian cell linesPrecise molecular mechanismsFMDV capsid proteinsNatural host cellsInhibition of autophagyCloven-hoofed animalsAutophagy inductionMolecular mechanismsAutophagy processHost cellsAkt-mTORMouth disease virusCapsid proteinViral replicationAutophagyFMDV replicationHSPB1InfectionCell linesVP2
2017
Sophoraflavenone G Restricts Dengue and Zika Virus Infection via RNA Polymerase Interference
Sze A, Olagnier D, Hadj SB, Han X, Tian XH, Xu HT, Yang L, Shi Q, Wang P, Wainberg MA, Wu JH, Lin R. Sophoraflavenone G Restricts Dengue and Zika Virus Infection via RNA Polymerase Interference. Viruses 2017, 9: 287. PMID: 28972551, PMCID: PMC5691638, DOI: 10.3390/v9100287.Peer-Reviewed Original ResearchConceptsZika virus infectionHepatitis C virusHepatitis C replicationPost-infection treatmentGlobal health concernAntiviral treatmentC virusVirus infectionVesicular stomatitis virusZika virusAntiviral compoundsHealth concernChinese medicinePolymerase inhibitionDengueStomatitis virusVirusTreatmentRNA polymerase inhibitionInfectionDiseaseFlavivirusesAn essential role of PI3K in the control of West Nile virus infection
Wang L, Yang L, Fikrig E, Wang P. An essential role of PI3K in the control of West Nile virus infection. Scientific Reports 2017, 7: 3724. PMID: 28623344, PMCID: PMC5473900, DOI: 10.1038/s41598-017-03912-5.Peer-Reviewed Original ResearchConceptsWest Nile virus infectionPI3K inhibitorsPI3KVirus infectionImmune responseK inhibitorsType I IFN responseAntiviral immune responseI IFN responseCatalytic subunit p110δTNF-α protein productionPrimary mouse macrophagesFlaviviral infectionsAntiviral immunityIFN responseViral titersClass I PI3KAntiviral roleMRNA expressionPI3K activityIFNProtein expressionInfectionMouse macrophagesCell proliferation
2016
Flavivirus NS1 protein in infected host sera enhances viral acquisition by mosquitoes
Liu J, Liu Y, Nie K, Du S, Qiu J, Pang X, Wang P, Cheng G. Flavivirus NS1 protein in infected host sera enhances viral acquisition by mosquitoes. Nature Microbiology 2016, 1: 16087. PMID: 27562253, PMCID: PMC5003325, DOI: 10.1038/nmicrobiol.2016.87.Peer-Reviewed Original ResearchConceptsNonstructural protein 1Japanese encephalitis virus nonstructural protein 1Dengue virusDENV nonstructural protein 1Flavivirus nonstructural protein 1Lethal DENV challengeNS1 proteinReceptor-deficient miceVirus nonstructural protein 1Flavivirus NS1 proteinAcquisition of virusDENV challengeActive immunizationFlaviviral diseasesImmune barrierHost serumViral transferInfected mammalian hostsViral acquisitionMosquito midgutMiceProtein 1Virus acquisitionImmunizationMosquito vectors
2015
A Neuron-Specific Antiviral Mechanism Prevents Lethal Flaviviral Infection of Mosquitoes
Xiao X, Zhang R, Pang X, Liang G, Wang P, Cheng G. A Neuron-Specific Antiviral Mechanism Prevents Lethal Flaviviral Infection of Mosquitoes. PLOS Pathogens 2015, 11: e1004848. PMID: 25915054, PMCID: PMC4411065, DOI: 10.1371/journal.ppat.1004848.Peer-Reviewed Original ResearchMeSH KeywordsAedesAnimalsAntiviral AgentsApoptosisBrainCell LineCell MembraneDengue VirusDrosophila melanogasterDrosophila ProteinsEncephalitis Virus, JapaneseFemaleHost-Pathogen InteractionsHumansInsect ProteinsNerve Tissue ProteinsNeuronsPhylogenyProtein Interaction Domains and MotifsRecombinant ProteinsViral Envelope ProteinsVirus InternalizationConceptsMosquito nervous systemCentral nervous systemJapanese encephalitis virusNervous systemFlaviviral infectionsDengue virusSindbis virusAntiviral mechanismMembrane targetingDramatic pathologyPlasma membraneAnti-flavivirus activityMosquito cellsSurface envelope proteinMosquito lifespanNatural vectorCulex pipiensHuman viral diseasesNeural cellsFlavivirus infectionEnvelope proteinFunctional blockadeNeural apoptosisMosquito behaviorViral infection
2013
Mosquito Saliva Serine Protease Enhances Dissemination of Dengue Virus into the Mammalian Host
Conway MJ, Watson AM, Colpitts TM, Dragovic SM, Li Z, Wang P, Feitosa F, Shepherd DT, Ryman KD, Klimstra WB, Anderson JF, Fikrig E. Mosquito Saliva Serine Protease Enhances Dissemination of Dengue Virus into the Mammalian Host. Journal Of Virology 2013, 88: 164-175. PMID: 24131723, PMCID: PMC3911723, DOI: 10.1128/jvi.02235-13.Peer-Reviewed Original ResearchConceptsDengue virusDENV infectivityAedes aegypti salivaMurine lymph nodesSalivary gland extractsAegypti salivaPrevention of diseaseLymph nodesMosquito salivaDENV loadVivo modelSerine protease activityNovel targetViral attachmentViral infectivityVirus infectivitySerine protease inhibitorHeparan sulfate proteoglycanExtracellular matrix proteinsRNA knockdownProtease inhibitorsInfectivitySulfate proteoglycanCell migrationMammalian hosts
2011
ISG15 facilitates cellular antiviral response to dengue and west nile virus infection in vitro
Dai J, Pan W, Wang P. ISG15 facilitates cellular antiviral response to dengue and west nile virus infection in vitro. Virology Journal 2011, 8: 468. PMID: 21992229, PMCID: PMC3215395, DOI: 10.1186/1743-422x-8-468.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell LineCytokinesDengue VirusMacrophagesMiceUbiquitinsVirus ReplicationWest Nile virusConceptsWest Nile virusNon-infected cellsWNV infectionViral infectionWest Nile virus infectionWest Nile meningoencephalitisInterferon beta 1Type I interferonCellular antiviral responseVirus infectionI interferonAntiviral responseFlaviviridae familyRAW264.7 cellsDENVInfectionConclusionsThese findingsBeta 1Protein ISGylationGene 15SOCS3 siRNANile virusCausative agentExact roleISG15Alterations in the Aedes aegypti Transcriptome during Infection with West Nile, Dengue and Yellow Fever Viruses
Colpitts TM, Cox J, Vanlandingham DL, Feitosa FM, Cheng G, Kurscheid S, Wang P, Krishnan MN, Higgs S, Fikrig E. Alterations in the Aedes aegypti Transcriptome during Infection with West Nile, Dengue and Yellow Fever Viruses. PLOS Pathogens 2011, 7: e1002189. PMID: 21909258, PMCID: PMC3164632, DOI: 10.1371/journal.ppat.1002189.Peer-Reviewed Original ResearchConceptsGene expressionDiverse cellular processesPupal cuticle proteinsExpression of genesMosquito gene expressionYellow fever virusFlaviviral infectionsMosquito genesCuticle proteinsCellular processesBioinformatics analysisMosquito cellsExpression profilesMicroarray analysisDDR genesMetabolic processesHuman diseasesGenesTranscriptomic signaturesWest NileFever virusPeptidase activityWNV envelope proteinTranscriptomeAedes aegypti mosquitoes