2020
A mosquito salivary protein promotes flavivirus transmission by activation of autophagy
Sun P, Nie K, Zhu Y, Liu Y, Wu P, Liu Z, Du S, Fan H, Chen CH, Zhang R, Wang P, Cheng G. A mosquito salivary protein promotes flavivirus transmission by activation of autophagy. Nature Communications 2020, 11: 260. PMID: 31937766, PMCID: PMC6959235, DOI: 10.1038/s41467-019-14115-z.Peer-Reviewed Original ResearchConceptsBeclin-1Viral transmissionFlavivirus transmissionMosquito salivary proteinsHost immune cellsZika virus transmissionActivation of autophagyLow viremiaProphylactic targetsMosquito salivaImmune cellsZIKV transmissionAllergen 1Infected mosquitoesViral infectionMonocyte lineageVirus transmissionMiceMosquitoesSalivary proteinsNumerous studiesViremiaInfectionFlavivirusesProtein
2015
A Neuron-Specific Antiviral Mechanism Prevents Lethal Flaviviral Infection of Mosquitoes
Xiao X, Zhang R, Pang X, Liang G, Wang P, Cheng G. A Neuron-Specific Antiviral Mechanism Prevents Lethal Flaviviral Infection of Mosquitoes. PLOS Pathogens 2015, 11: e1004848. PMID: 25915054, PMCID: PMC4411065, DOI: 10.1371/journal.ppat.1004848.Peer-Reviewed Original ResearchMeSH KeywordsAedesAnimalsAntiviral AgentsApoptosisBrainCell LineCell MembraneDengue VirusDrosophila melanogasterDrosophila ProteinsEncephalitis Virus, JapaneseFemaleHost-Pathogen InteractionsHumansInsect ProteinsNerve Tissue ProteinsNeuronsPhylogenyProtein Interaction Domains and MotifsRecombinant ProteinsViral Envelope ProteinsVirus InternalizationConceptsMosquito nervous systemCentral nervous systemJapanese encephalitis virusNervous systemFlaviviral infectionsDengue virusSindbis virusAntiviral mechanismMembrane targetingDramatic pathologyPlasma membraneAnti-flavivirus activityMosquito cellsSurface envelope proteinMosquito lifespanNatural vectorCulex pipiensHuman viral diseasesNeural cellsFlavivirus infectionEnvelope proteinFunctional blockadeNeural apoptosisMosquito behaviorViral infection
2011
Alterations in the Aedes aegypti Transcriptome during Infection with West Nile, Dengue and Yellow Fever Viruses
Colpitts TM, Cox J, Vanlandingham DL, Feitosa FM, Cheng G, Kurscheid S, Wang P, Krishnan MN, Higgs S, Fikrig E. Alterations in the Aedes aegypti Transcriptome during Infection with West Nile, Dengue and Yellow Fever Viruses. PLOS Pathogens 2011, 7: e1002189. PMID: 21909258, PMCID: PMC3164632, DOI: 10.1371/journal.ppat.1002189.Peer-Reviewed Original ResearchConceptsGene expressionDiverse cellular processesPupal cuticle proteinsExpression of genesMosquito gene expressionYellow fever virusFlaviviral infectionsMosquito genesCuticle proteinsCellular processesBioinformatics analysisMosquito cellsExpression profilesMicroarray analysisDDR genesMetabolic processesHuman diseasesGenesTranscriptomic signaturesWest NileFever virusPeptidase activityWNV envelope proteinTranscriptomeAedes aegypti mosquitoesAn In Vivo Transfection Approach Elucidates a Role for Aedes aegypti Thioester-Containing Proteins in Flaviviral Infection
Cheng G, Liu L, Wang P, Zhang Y, Zhao YO, Colpitts TM, Feitosa F, Anderson JF, Fikrig E. An In Vivo Transfection Approach Elucidates a Role for Aedes aegypti Thioester-Containing Proteins in Flaviviral Infection. PLOS ONE 2011, 6: e22786. PMID: 21818390, PMCID: PMC3144946, DOI: 10.1371/journal.pone.0022786.Peer-Reviewed Original ResearchMeSH KeywordsAedesAnimalsDengueDengue VirusEstersGene SilencingGreen Fluorescent ProteinsInsect ProteinsPlasmidsRNA, MessengerTransfection
2010
A C-Type Lectin Collaborates with a CD45 Phosphatase Homolog to Facilitate West Nile Virus Infection of Mosquitoes
Cheng G, Cox J, Wang P, Krishnan MN, Dai J, Qian F, Anderson JF, Fikrig E. A C-Type Lectin Collaborates with a CD45 Phosphatase Homolog to Facilitate West Nile Virus Infection of Mosquitoes. Cell 2010, 142: 714-725. PMID: 20797779, PMCID: PMC2954371, DOI: 10.1016/j.cell.2010.07.038.Peer-Reviewed Original ResearchMeSH KeywordsAedesAnimalsCulexHumansInsect ProteinsLectins, C-TypeLeukocyte Common AntigensVirus InternalizationWest Nile virusConceptsWest Nile virusWNV infectionWest Nile virus infectionArthropod-borne flavivirusBlood-feeding processVirus infectionHuman CD45Viral disseminationC-type lectinInfectionViral entryViral attachmentMosquito homologCalcium-dependent mannerNile virusMolecular understandingVivo experimentsSame pathwayNatural vector