Lentivirus-based genetic manipulations of cortical neurons and their optical and electrophysiological monitoring in vivo
Dittgen T, Nimmerjahn A, Komai S, Licznerski P, Waters J, Margrie TW, Helmchen F, Denk W, Brecht M, Osten P. Lentivirus-based genetic manipulations of cortical neurons and their optical and electrophysiological monitoring in vivo. Proceedings Of The National Academy Of Sciences Of The United States Of America 2004, 101: 18206-18211. PMID: 15608064, PMCID: PMC539748, DOI: 10.1073/pnas.0407976101.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCalcium-Calmodulin-Dependent Protein Kinase Type 2Calcium-Calmodulin-Dependent Protein KinasesElectrophysiologyGenetic VectorsGreen Fluorescent ProteinsHippocampusImage Processing, Computer-AssistedLentivirusMiceMice, Inbred C57BLMicroscopyNeuronsPhenotypePhotonsPromoter Regions, GeneticRecombinant ProteinsRNA, Small InterferingRNA, Small NuclearSynapsinsConceptsU6 promoter-driven expressionMicroscopy-based techniquesGenetic manipulationGene deliveryLentiviral vectorsGene functionGene knockdownSubsequent phenotypic analysisPromoter-driven expressionCellular response propertiesSmall networksTwo-photon microscopyTwo-photon time-lapse imagingCortical neuronsEfficient meansMicroscopyAlpha-calcium/calmodulin-dependent protein kinase IIPostsynaptic excitability is necessary for strengthening of cortical sensory responses during experience-dependent development
Komai S, Licznerski P, Cetin A, Waters J, Denk W, Brecht M, Osten P. Postsynaptic excitability is necessary for strengthening of cortical sensory responses during experience-dependent development. Nature Neuroscience 2006, 9: 1125-1133. PMID: 16921372, DOI: 10.1038/nn1752.Peer-Reviewed Original ResearchConceptsSomatodendritic excitabilityExperience-dependent developmentLayer 2/3 pyramidal neuronsCortical networksSensory responsesRat somatosensory cortexNormal cortical developmentCortical sensory responsesDevelopmental strengtheningPostsynaptic excitabilityPyramidal neuronsSomatosensory cortexCortical neuronsBarrel cortexPostsynaptic neuronsCortical developmentSensory cortexSensory pathwaysSensory responsivenessSynaptic strengthExcitabilitySensory deprivationCortexNeuronsVivo recordingsIncomplete and Inaccurate Vocal Imitation after Knockdown of FoxP2 in Songbird Basal Ganglia Nucleus Area X
Haesler S, Rochefort C, Georgi B, Licznerski P, Osten P, Scharff C. Incomplete and Inaccurate Vocal Imitation after Knockdown of FoxP2 in Songbird Basal Ganglia Nucleus Area X. PLOS Biology 2007, 5: e321. PMID: 18052609, PMCID: PMC2100148, DOI: 10.1371/journal.pbio.0050321.Peer-Reviewed Original ResearchConceptsDevelopmental verbal dyspraxiaVocal imitationKnockdown of FoxP2Comprehension of grammarVocal motor learningSong learningWord productionMutations of FOXP2Neural parallelsLanguage disordersHuman speechHuman languageMotor learningFluent productionVerbal dyspraxiaSong ontogenyTutor songSpeechImitationBasal ganglia structuresAcoustic structureLearningGanglia structuresBasal gangliaFoxP2 levels