2009
The structure of the ankyrin-binding site of β-spectrin reveals how tandem spectrin-repeats generate unique ligand-binding properties
Stabach PR, Simonović I, Ranieri MA, Aboodi MS, Steitz TA, Simonović M, Morrow JS. The structure of the ankyrin-binding site of β-spectrin reveals how tandem spectrin-repeats generate unique ligand-binding properties. Blood 2009, 113: 5377-5384. PMID: 19168783, PMCID: PMC2689040, DOI: 10.1182/blood-2008-10-184291.Peer-Reviewed Original ResearchAlanineAmino Acid MotifsAmino Acid SequenceAnkyrinsBinding SitesCrystallography, X-RayHumansLigandsMechanotransduction, CellularModels, MolecularMolecular Sequence DataMutagenesis, Site-DirectedProtein BindingProtein FoldingProtein Interaction MappingProtein Structure, TertiaryRepetitive Sequences, Amino AcidSequence Homology, Amino AcidSpectrin
1997
Site-Directed Mutagenesis of αII Spectrin at Codon 1175 Modulates Its μ-Calpain Susceptibility †
Stabach P, Cianci C, Glantz S, Zhang Z, Morrow J. Site-Directed Mutagenesis of αII Spectrin at Codon 1175 Modulates Its μ-Calpain Susceptibility †. Biochemistry 1997, 36: 57-65. PMID: 8993318, DOI: 10.1021/bi962034i.Peer-Reviewed Original ResearchConceptsSite-directed mutagenesisAlpha II spectrinCalpain cleavage sitesCleavage siteII-spectrinHelix CRecombinant GST-fusion proteinsBona fide proteinGST fusion proteinTriple-helical motifsStrict substrate specificityFamily of Ca2Protein kinase CDynamic molecular modelingStructural repeatsProminent substrateDifferent amino acidsSubstrate specificityIntracellular proteolysisPenultimate residueCysteine proteasesKinase CMost proteasesSteroid receptor activationSpectrin